• Shaimaa Elbaz
  • Eldsokey Nassef
  • Abdelnasser Bakr
  • Elsayed Hegazi
  • Abeer EL-keredy



The aim of current study was to determine the effect of dietary omega 3 fatty acids (FA) mainly eicosapentaenoic (EPA) and docosahexaenoic (DHA) on inflammatory response of post calving cows during transition to lactation. Twenty Holstein lactating cows were assigned into two groups (10 each). The two groups fed on the basal diet while one of them was supplemented with 70 g of omega-3 FA per cow. The supplement was added to the diet from the 1ST day of calving till the 60th days of lactation. Blood samples were taken 3 times weekly from the 2nd to the 4th week post calving. The blood samples were analyzed for glucose, insulin, non-esterified fatty acids (NEFA), beta hydroxyl butyric acid (BHBA), C- reactive protein (CRP), haptoglobin, albumin and globulin. Feeding omega-3 FAs significantly increased (P<0.05) blood glucose level by 17.35%, 28.23% and 24.73% than the control at weeks 1, 2, 3 of the experiment, respectively. BHBA was lower in cows fed omega-3 at 1st and 2nd week then became nearly similar to the control at the end of experiment. At 1st and 2nd week, cows fed omega-3 FAs had significantly lower serum CRP than those fed the control diet and became nearly similar to control at the end of the experiment. Serum, globulin was significantly higher in cows fed omega 3 FAs at 2nd and 3rd week by 81.1% and 51.2%, respectively. Omega 3 FA improved energy status of fresh cow by increasing serum insulin and glucose levels while decreasing NEFA and BHBA parameters. Also, feeding omega 3 FA improved humoral immunity through increasing serum globulin and reduced inflammatory response by lowering CRP post calving.

Key words: omega 3 fatty acids; lactating cow; inflammation; energy balance; serum protein


Piccione G, MessinaV, Marafioti S, Casel-la S, Giannetto C and Faziof. Changes of some haemato chemical parameters in dairy cows during late gestation, postpartum, lactation and dry periods. Vet. Med. Zoot. 2012; 58, 59–64.

Wathes DC, Cheng Z, Chowdhury W , Fenwick MA , Fitzpatrick R, Morris DG, Patton J and Murphy JJ. Negative energy balance alter global gene expression and immune responses in the uterus of postpartum dairy cows. Physiol Genomics, T. 2009; 39:1–13.

Humblet MF, Guyot H,Boudry B, Mba-yahi F, Hanzen C, Rollin F, and Godeau JM. Relationship between haptoglobin, serum amy-loid A, and clinical status in a survey of dairy herds during a 6-month period.Vet. Clin. Pathol. 2006; 35:188–93.

Scalia D, Lacetera N, Bernabucci U, Demeyere K, Duchateau L and Burvenich C. Invitro effects of nonesterified fatty acids on bovine neutrophils oxidative burst and viabil-ity.J. Dairy Sci.2006; 89: 147–54.

Contreras GA, Rephael WS, Mattmiller A, Gandy J, and Sordillo LM. Non esterified fatty acids modify inflammatory response and eico-sanoid biosynthesis in bovine endothelial cells. J. Dairy sci.2012b; 95:5011–23.

Silvestre F, Carvalho PC, Crawford JE, Santos P, Staples CR, Jenkins T, and Thatcher WW. Effects of differential supplementation of fatty acids during the peri partum and breeding periods of Holstein cows: II .Neutrophil fatty acids and function, and acute phase proteins .J. Dairy sci.2011; 94:2285–301.

Calder PC, Yaqoop, Thies F, Wallace FA, Miles EA .Fatty acids and lymphocyte functions.Br.J.Nutr.2002; 87:S31-48.

Clarke SD. The multi-dimensional regula-tion of gene expression by fatty acids: poly unsaturated fats as nutrient sen-sors.Curr.opin.Lipid.2004; 15:13–8.

Lee JY, Zhao L, and Hwang DH. Modula-tion of pattern recognition receptor-mediated inflammation and risk of chronic diseases by dietary fatty acids.Nutr.Rev.2010; 68:38–61.

Oh DY, Talukdar S, Bae EJ, Imamure T, Morinaga H, Fan W , Li P, Lu WJ, Watkins SM, and Olefsjy JM.GPR120 IS an omega-3 fatty acid Receptor Mediating Potent Anti-Inflammatory and insulin-sensitizing Effects. Cell .2010; 142 (5):687–98.

Calder PC. Mechanisms of action of (n-3) fatty acids. J. Nutr. 2012; 142: 5925–95.

Gandra JR, Barletta RV , Mingoti RD, Verdurico LC, Freitas JE , Oliveira L J ,Takiya CS, Kfoury JR, Wiltbank MC, and Renno FP. Effects of whole flaxseed ,raw soybeans ,and calcium salts of fatty acids on measures of cellular immune function of transition dairy cows .J. Dairy Sci. 2016a; 99:4590–606

Dirabdeh E,Towhidi A, Zein oaldin IS, Ganj khanoul M,Ansaripirsaraei Z,Fouladi-Nashta A.Effects of different polyunsaturated fatty acids supplementations during the postpar-tum periods of early lactating dairy cows on milk yield ,metabolic responses, and reproduc-tive performances. J. Anim.Sci.2013; 91:713–21.

Gifford CA, Holland BP, Mills RL, Maxwell CL, Farney JK, Terrill SJ, Step DL, Richards CJ, Burciaga Robles LO, and Krehible CR. Impacts of inflammation on cattle growth and Carcass merit. J. amin. Sci. 2012; 90:1438–51.

National Research Council .Nutrients of Dairy Cattle .7th Rev. Ed.Natl. ACAD.Sci.2001; Washington, DC.

Associations of Official Analytical Chemists .Official Methods of Analysis .16th Ed.AOAC1995; Arlington .VA.

Vansoest PJ, Robert son JB and Iewis BA. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition .J. Dairy .Sci.1991; 74:3583–97.

Hintz RW and Mertens DR. Effects of sodium sulfite on recovery and composition of detergent fiber and lignin. J.AOAC Int.1996; 79: 16.

Association of Official Analytical Chem-ists. Official Methods of Analysis 1991; 74:81.

Abdellatif K R A, Fadaly W A A, Kamel G M, Elshaier Y A M M, El-Magd M A. Design, synthesis, modeling studies and biological eval-uation of thiazolidine derivatives containing pyrazole core as potential anti-diabetic PPAR-γ agonists and anti-inflammatory COX-2 selective inhibitors, Bioorganic Chemistry 2019; 82: 86–99.

Smith and Wilson. Free fatty Acids and Atherosclerosis. J. Clin .Endocrinal Metab 2006; 91: 2506–8.

Murray CH, Blanchflower WJ, and Rice DA.Clinical chemistry.3013, 421; 1984.

Burtis CA Tietz Text book of clinical chemistry, 3rd ed, AACC 1999.

Reinhold RR. Determination of serum albumin.clin.chem.1953; 21:1370-1372.

Coles EH: Vet. Clin.Path.1974; 211-213.W.B.Sounders Company Philadelphia, London,Toronto.

Chu CC, Lee SL and Fung HP. Purifica-tion, analysis and basal level of serum hapto-globin in cattle.P.88.In: Congress of the Taiwan Association of Veterinary Medicine and Animal Husbandry, Taiwan.2002.

Burtis CA, Ashwood ER,Titez Text book of clinical chemistry. 2nd Edition, W.B. Saun-ders, Philadelphia, PA (1994).

Eatham RD: Biochemical Values in Clin-ical Medicine, 7th Ed. Bristol. England .John Wright&sons,Ltd: 1985.

Humblet MF, Guyot H,Boudry B, Mba-yahi F, Hanzen C ,Rollin F,and Godeau JM. Relationship between haptoglobin, serum amy-loid A,and clinical status in a survey of dairy herds during a 6-month peri-od.Vet.Clin.Pathol.2006;35:188–93.

Pighin D, Kara batas L, Rossi A, Chicco A , Basabe JC, Lombardo YB,2003.Fish oil affects pancreatic fat storage, Pyruvate dehy-drogenase complex activity and insulin secre-tion in rats fed a sucrose –rich di-et.J.Nutr.2003;133:4095–101.

Juchem SO, Santos JEP, Cerri RLA, Chebel RC, Galvao KN, Bruno R, Depeters EJ, Scott T , Thatcher WW , Luchini D. Effect of calcium salts of fish and palm oils on lactational performance of Holstein cows .Ani.feed .Sci.2008;140:18–38.

Gandra JR, , Mingoti RD, Barletta RV , Takiya CS , Verdurico LC, Freitas JE ,Paiva PG, Jesus EF, Calomeni GD and Renno FP . Effects of flaxseed ,raw soybeans ,and calcium salts of fatty acids on apparent total tract digest-ibility, energy balance and milk fatty acid pro-file of transition cows .Animal.2016b;1:1–8.

Mattos R, Staples CR, Arteche A, Wilt-bank MC, Diaz FJ, Jenkins TC and Thatcher WW. The effects of feeding fish oil on uterine secretion of PGF2α, Milk composition, and metabolic status of periparturient Holstein cows.J.Dairy .Sci.2004; 87:921 –32.

Heravi Moussavi AR, Gilbert RO, Verton TRO, Bauman DE and Butler WR. Effects of feeding fish meal and n-3fatty acids on milk yield and metabolic responses in early lactating dairy cows J.Dairy sci. 2007; 90:136–44.

Bilby TR, Sozzi A, Lopez MM, Silvestre FT, Ealy AD, Staples CR& Thatcher WW. Pregnancy , bovine somatotropin, and dietary n-3 fatty acids in lactating dairy cow: I. Ovarian, conceptus, and growth hormone – insulin –like growth factor system responses .Journal of dairy Sci. 2006b; 89: 3360–74.

Drackley JK. Biology of dairy cows dur-ing the transition period; the final frontier? J. Dairy Sci.1999; 82:2259–73.

Greco LF, Neves Neto JT, Pedrico A, Ferraza RA, Lima FS, Bisinotto RS, Martinez N, Garcia M, Ribeiro ES, Gomes GC, Shin JH, Ballas MA, Thatcher WW, Staples CR, and San-tos JEP. Effects of altering the ratio of dietary n-6 ton-3 fatty acids on performance and in-flammatory responses to a lipopolysaccharide challenge in lactating Holstein cows.J.Dairy Sci.2015;98:602–17.

Gonthier C, Mustafa AF, Ouellet DR, Chouinard PY, Berthiaume R, and Petit HV. Feeding Micronized and extruded flaxseed to dairy cows. Effects on Blood parameters and milk fatty acid composition. J.Dairy Sci.2005; 88:746–56.

Elis S, Freret S, Desmarchais A, Maillard V, Cognie J, Briant E, Touze JL, Dupont M, Faverdin P, Chajes V, Uzbekova S, Monget P and Dupont J. Effect of a long chain n-3PUFA-enriched diet on production and reproduction variables in Holstein dairy cows. Animal Re-production Sci.2016; 164:121–32.

Ulfina GG, Kimotni SP,Oberoi PS, Baithalu RK, Kumaresan A, Moohanty TK, Imtiwati P and Dang AK. Modulation of post-partum reproductive performance in dairy cows through supplementation of long-or short –chain fatty acids during transition period JPn.2015;99,1056–64.

Marinkovic S, Jahreis GP, Wong GG and Baumann H. IL-6 modulates the synthesis of a specific set of acute phase plasma proteins in vivo .J.Immunol.1989; 142:808–12.

Kent JE. Acute Phase Proteins: their use in Veterinary diagnosis; 148:279–82.

Staples CR, Amaral B, Silvestre F, Cal-dari Torres C, Cullens FM, Badinga L, Arthing-ton JD and Thatcher WW .2008.Immune system responses to diseases /Disorders in the dairy animal and potential effects of essential fatty acids Florida Ruminant Nut.symp.2008;F1 32611,352–92.

Nazifi S, Rezak hani A, Koohi-moghadam M, Ansari-Lari M and Esmailnezhad Z. Evaluation of scrum haptoglobin in clinically healthy cattle and cattle with inflammatory dis-eases in Shiraz, a tropical area in Southern 2008;11,No2,95-101




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Veterinary medicine: Research and application