• Mahmoud Dawood
  • Mohamed Mohsen
  • Ashraf El-dakar
  • Elsaid Abdelraouf
  • Eman Moustafa
  • Hamada Ahmed




The current study was established to test the possibility of using exogenous digestive enzymes (EDE) in rabbitfish (Siganus rivulatus) diets. Five experimental diets containing 34% soybean meal were prepared and supplemented with EDE at 0, 1, 2, 3, and 4 g kg-1 diet. Fish with average initial weight (± 1.1 g) were fed the test diets for 74 days. Rabbitfish offered the diets with EDE supplements exhibited significantly (P<0.05) higher growth performance, feed intake, protein efficiency ratio, protein gain, and protein retention over the basal diet. But, no significant (P>0.05) differences were observed in carcass composition and somatic parameters index with EDE supplementation. The physiological condition of fish fed diets with EDE showed that the hematocrit (%), total plasma protein and total plasma globulin were significantly (P<0.05) higher in fish fed EDE than the control group. It can be concluded that the addition of EDE in rabbitfish diets improved the growth performance, feed efficiency and health condition.

Key words: blood parameters; exogenous digestive enzymes; feed efficiency; growth; rabbitfish


Dawood MAO, Koshio S. Recent advances in the role of probiotics and prebiotics in carp aquaculture: a review. Aquaculture 2016; 454:243–51.

Dawood MAO, El-Dakar A, Mohsen M, Abdelraouf E, Koshio S, Ishikawa M, Yokoyama S. Effects of using exogenous digestive enzymes or natural enhancer mixture on growth, feed utilization, and body composition of Rabbitfish, Siganus rivulatus. Journal of Agricultural Science and Technology. B. 2014;4(3B).

Goda AM, Mabrouk HA, Wafa MA, El-Afifi TM. Effect of using baker's yeast and exogenous digestive enzymes as growth promoters on growth, feed utilization and hematological indices of Nile tilapia, Oreochromis niloticus fingerlings. Journal of Agricultural Science and Technology. B. 2012;2(1B).

Dawood MA, Koshio S, Ishikawa M, Yokoyama S. Effects of partial substitution of fish meal by soybean meal with or without heat-killed Lactobacillus plantarum (LP20) on growth performance, digestibility, and immune response of amberjack, Seriola dumerili juveniles. BioMed Res Int 2015;2015.

Dossou S, Koshio S, Ishikawa M, Yokoyama S, Dawood MAO, El Basuini MF, El-Hais AM, Olivier A. Effect of partial replacement of fish meal by fermented rapeseed meal on growth, immune response and oxidative condition of red sea bream juvenile, Pagrus major. Aquaculture 2018; 490:228–35.

Dossou S, Koshio S, Ishikawa M, Yokoyama S, Dawood MAO, El Basuini MF, Olivier A, Zaineldin AI. Growth performance, blood health, antioxidant status and immune response in red sea bream (Pagrus major) fed Aspergillus oryzae fermented rapeseed meal (RM-Koji). Fish Shellfish Immunol 2018; 75:253–62.

Dossou S, Koshio S, Ishikawa M, Yokoyama S, El Basuini MF, Zaineldin AI, Mzengereza K, Moss A, Dawood MAO. Effects of replacing fishmeal with fermented and non-fermented rapeseed meal on the growth, immune and antioxidant responses of red sea bream (Pagrus major). Aquacult Nutr 2019; https://online library.wiley.com/doi/abs/10.1111/anu.12876

Nordrum S, Bakke-McKellep AM, Krogdahl Å, Buddington RK. Effects of soybean meal and salinity on intestinal transport of nutrients in Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol B Biochem Mol Biol 2000; 125(3):317–35.

Yigit NO, Keser E. Effect of cellulase, phytase and pectinase supplementation on growth perfo-rmance and nutrient digestibility of rainbow trout (Oncorhynchus mykiss, Walbaum 1792) fry fed diets containing canola meal. J Appl Ichthyol 2016;32(5):938–42.

Feord JC. Exogenous enzymes improve performance of carp and tilapia when fed diets containing high levels of soybean meal, in: VII International Symposium on Nutrition and Feeding of Fish. 1996.

Forster I, Higgs DA, Dosanjh BS, Rowshandeli M, Parr J. Potential for dietary phytase to improve the nutritive value of canola protein concentrate and decrease phosphorus output in rainbow trout (Oncorhynchus mykiss) held in 11 °C fresh water. Aquaculture 1999; 179(1-4):109–25.

Johnson R, Williams P, Campbell R. Use of enzymes in pig production. Proc. 1st Symp. On Enzymes in Animal Nutrition (Ed. C. Wenk and M. Boessinger). 1993: 49–60.

El-Dakar AY, Shalaby SM, Saoud IP. Assessing the use of a dietary probiotic/prebiotic as an enhancer of spinefoot rabbitfish Siganus rivulatus survival and growth. Aquacult Nutr 2007;13(6):407-12.

Pillay TVR, Kutty MN. Aquaculture: Principles and Practices, 2nd edn. Blackwell Publishing, Ames, IA, USA, pp 2005:458–60.

Dawood MAO, Koshio S, Abdel-Daim MM, Van Doan H. Probiotic application for sustainable aquaculture. Rev Aquacult 2018. https://onlinelibrary.wiley.com/doi/abs/10.1111/raq.12272

Dawood MAO, Koshio S, Esteban MÃ. Beneficial roles of feed additives as immunostimulants in aquaculture: a review. Rev Aquacult 2018; 10(4):950–74.

Dawood MAO, Koshio S, Ishikawa M, Yokoyama S. Effects of heat killed Lactobacillus plantarum (LP20) supplemental diets on growth performance, stress resistance and immune response of red sea bream, Pagrus major. Aquaculture 2015; 442:29–36.

Coles EH. Plasma Proteins, Veterinary Clinical Pathology, 2nd ed., W. B. Saunders Co., London. pp. 1974:558-560.

AOAC. Official Methods of Analysis of Association of Official Analytical Chemists, 15th edn. Published by the Association of Analytical Chemists, Arlington, VA, USA.1990.

NRC. Nutrition Requirements of Fish. National Academy Press, Washington, DC, USA. 1993.

Garling Jr DL, Wilson RP. Optimum dietary protein to energy ratio for channel catfish fingerlings, Ictalurus punctatus. J Nutr 1976; 106(9):1368–75.

Kolkovski S, Tandler A, Izquierdo MS. Effects of live food and dietary digestive enzymes on the efficiency of microdiets for seabass (Dicentrarchus labrax) larvae. Aquaculture 1997; 148(4):313–22.

Jackson LS, Li MH, Robinson EH. Use of Microbial Phytase in Channel Catfish Ictalurus punctatus Diets to Improve Utilization of Phytate Phosphorus 1. J World Aquacult Soc 1996; 27(3):309–13.

Debnath D, Sahu NP, Pal AK, Jain KK, Yengkokpam S, Mukherjee SC. Mineral status of Pangasius pangasius (Hamilton) fingerlings in relation to supplemental phytase: absorption, wholeâ€body and bone mineral content. Aquacult Res 2005;36(4):326–35.

Giri SS, Sahoo SK, Sahu AK, Meher PK. Effect of dietary protein level on growth, survival, feed utilisation and body composition of hybrid Clarias catfish (Clarias batrachus× Clarias gariepinus). Animal Feed Sci Tech 2003;104(1-4):169–78.

Ali HM, Ghazalah AA, Gehad EA, Hammouda YA, Abo-State HA. Practical aspects and immune response of probiotics preparations supplemented to Nile tilapia (Oreochromis niloticus) diets. Nature & Science 2010; 8:39–45.

El-Haroun ER, Goda AS, Kabir Chowdhury MA. Effect of dietary probiotic Biogen® supplementation as a growth promoter on growth performance and feed utilization of Nile tilapia Oreochromis niloticus (L.). Aquacult Res 2006; 37(14):1473–80.

Lin S, Mai K, Tan B. Effects of exogenous enzyme supplementation in diets on growth and feed utilization in tilapia, Oreochromis niloticus x O. aureus. Aquacult Res 2007;38(15):1645–53.

Irianto A, Austin B. Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis 2002;25(6):333–42.

Singh P, Maqsood S, Samoon MH, Phulia V, Danish M, Chalal RS. Exogenous supplementation of papain as growth promoter in diet of fingerlings of Cyprinus carpio. International Aquatic Research 2011; 3:1–9.

McAllister TA, Hristov AN, Beauchemin KA, Rode LM, Cheng, KJ. Enzymes in ruminant diets. In: Bedford, M., Partridge, G. (Eds.), Enzymes in Farm Animal Nutrition. CABI Publishing, Oxon, UK, 2001; 273–98.

Shalaby SM, El-Dakar AY, Ghoneim SI. Protein sparing effect by carbohydrate in diets of rabbitfish, Siganus rivulatus. Egyptian Journal of the Aquatic Biology and Fisheries 2001; 5:87–98.

Wong MH, Tang LY, Kwok FS. The use of enzyme-digested soybean residue for feeding common carp. Biomedical and environmental sciences: BES. 1996;9(4):418–23.

Chakrabarti I, Gani MA, Chaki KK, Sur R, Misra KK. Digestive enzymes in 11 freshwater teleost fish species in relation to food habit and niche segregation. Comp Biochem Physiol A Mol Integr Physiol 1995 Sep; 112(1):167–77.

Moraes G, Bidinotto PM. Induced changes in the amylohydrolytic profile of the gut of Piaractus mesopotamicus (Holmberg, 1885) fed different levels of soluble carbohydrate: its correlation with metabolic aspects. Revista de Ictiologia. 2000;8(1/2):47–51.

Lundberg B, Lipkin Y. Natural food of the herbivorous rabbitfish (Siganus spp.) in northern Red Sea. Botanica Marina 1979; 22(3):173–82.

Kawai S. Studies on digestive enzymes of fishes-III. Development of digestive enzyme of rainbow trout after hatching and the effect of dietary change on the activities of digestive enzyme in the juvenile stage. Bull Jpn Soc Sci Fish 1973; 39:819–27.

Sabapathy U, Teo LH. A quantitative study of some digestive enzymes in the rabbitfish, Siganus canaliculatus and the sea bass, Lates calcarifer. J Fish Biol 1993;42(4):595–602.

Lara-Flores M, Olvera-Novoa MA, Guzmán-Méndez BE, López-Madrid W. Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus). Aquaculture 2003;216(1-4):193–201.

Dawood MAO, Koshio S, Ishikawa M, Yokoyama S. Interaction effects of dietary supplementation of heat-killed Lactobacillus plantarum and β-glucan on growth performance, digestibility and immune response of juvenile red sea bream, Pagrus major. Fish Shellfish Immunol 2015; 45(1):33–42.

Dawood MAO, Koshio S, Ishikawa M, Yokoyama S. Immune responses and stress resistance in red sea bream, Pagrus major, after oral administration of heat-killed Lactobacillus plantarum and vitamin C. Fish Shellfish Immunol 2016;54:266–75.

Dawood MAO, Koshio S, Ishikawa M, Yokoyama S, El Basuini MF, Hossain MS, Nhu TH, Dossou S, Moss AS. Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major. Fish Shellfish Immunol 2016; 49:275–85.




How to Cite

Dawood, M., Mohsen, M., El-dakar, A., Abdelraouf, E., Moustafa, E., & Ahmed, H. (2019). EFFECTIVENESS OF EXOGENOUS DIGESTIVE ENZYMES SUPPLEMENTATION ON THE PERFORMANCE OF RABBITFISH (Siganus rivulatus). SLOVENIAN VETERINARY RESEARCH, 56(22-Suppl). https://doi.org/10.26873/SVR-779-2019



Veterinary Medicine and The One Health Concept