Nadia Mahfouz, Eman Moustafa, Mohamed Kassab, Wesam Marzouk


The present study was carried out to screen the predominant mycotic infections among freshwater fishes (Oreochromis niloticus and Clarias gariepinus) in Kafrelsheikh fish farms; with special focus on the seasonal incidence; as well as, the histopathological changes induced by the detected fungi. 500 specimens of freshwater fishes (400 O. niloticus and 100 C. gariepinus) were investigated for seasonal incidence of mycotic diseases. Mycological examination revealed the isolation of 2148 fungal isolates from 375 diseased and 125 apparently healthy fish samples (1828 mould and 320 yeast isolates), of which 1258 were isolated from O. niloticus and 890 isolates from C. gariepinus. Saprolegnia was the predominant among diseased fishes with highest prevalence in late autumn (10.68%, 6.96%) and winter (6.81%, 7.87%) in O. niloticus and C. gariepinus, respectively. However, Pencillium sp. and Aspergillus sp. were the most predominant fungi isolated from apparently healthy fishes. The highest prevalence of Pencillium  sp. were recorded in winter, whileas  Aspergillus showed variations between species; Aspergillus flavus, Aspergillus niger were more prevalent in summer (25.44%, 23.22%) and  (26.9%, 37.44%) and Aspergillus terrus, Aspergillus fumigatus were more prevalent in autumn (5.98%, 5.67%) and  (7.69%, 8.23%) in O. niloticus and  C. gariepinus, respectively. Moreover, the highest prevalence of Fusarium species was recorded in spring (11.8%, 5.91%) from O. niloticus, C. gariepinus, respectively. Mucor recorded the highest prevalence in autumn (20.09%) in O. niloticus and winter (29.21%) in C. gariepinus; whileas Rhizopus was highest in summer (7.89%, 5.21%) in O. niloticus and C. gariepinus, respectively. Four genera from yeast were isolated; Candida sp. (28.44%, 36.27%), Rhodotorula sp. (36.24%, 24.51%), Cryptococcus sp. (16.97%, 20.59%) and Trichosporon Sp. (18.35%, 18.63%) in O. niloticus and C. gariepinus, respectively. The histopathological findings revealed severe degenerative changes in skin and gills with presence of fungal hyphae and spores.

Key words: Clarias gariepinus; moulds; mycotic diseases; Oreochromis niloticus; yeast

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Hussain SM, Javed M, Javid A, Javid T, Hussain N. Growth responses of Catla catla, Labeo rohita and Cirrhina mrigala during chronic exposure of iron. Pak J Agric Sci. 2011;(1);48:225–30.

Mohamed HMA, Walaa FAE, Braeuning A, Hammad S. Detection of aflatoxin –producing fungi isolated from tilapia and fish feed. Excil J. 2017; 16:1308–18.

El-Sayed AFM, Dickson MW, El-Naggar GO. Value chain analysis of the aquaculture feed sector in Egypt. Aquac 2015; 437: 92–101.

FAO. Fishery and Aquaculture Statistics Yearbook 2014. Rome, Italy: Food and Agricul-ture organization of the United Nations. ISSN 2070-6057 (2016).

Nandlal S, Pickering T. Tilapia fish farm-ing in Pacific Island countries. Vol. 1: Tilapia hatchery operation. Noumea, New Caledonia: Secretariat of the Pacific Community, 2004.

Rodolphe EG, Marshall WL, Lilje O, Jes-sop CN, Gleason FH, Andreou D. Current eco-logical understanding of fungal-like pathogens of fish: what lies beneath?. HAL Id: hal-01321245 2014

Ashour AA, Mustafa SA, Yassein SN. Histopathological studies on common carp (Cy-prinus carpio L.) infected with Saprolegnia sp. and treated with Virkon®. Mirror Res. Vet. Sci. Anim. 2017; 6: 19–30.

Noor El-Deen A, Hussein M O, Zaki M S and Abo-State H A Mass Mortality in Cultured Nile Tilapia Oreochromis niloticus due to Saprolegniosis with Emphasis on Treatment Trials. J Biol Sci. 2018; 18(1):39–4

Refai MK, Laila K, Amany M, Shimaa ES. The assessment of Mycotic settlement of fresh-water fishes in Egypt. J Am Sci, 2010; 6(11): 823––31.‏

Pickering AD, Willoughby LG. Sapro-legnia infections of salmonid fish. In Microbial Diseases of Fish, Edit Roberts, RJ Academic Press, London, 1982, 271–98.

Chauhan R, Lone S, Beigh AH. Pathogencity of three species of Aspergullis (A. fumigatus, A. niger & A. sydowii) on some freshwater Fishes. Life Sci Leaflets, 2014; 14:65–72. (Online print).

West PV. Saprolegnia paracitica, an oomycyte pathogen with a fishy appetite: New challenges for an old problem, mycologist, 2006: 20(3):99–104.

Yanong RP. Fungal diseases of fish. Vet.

Clin. North Am. Exot. Anim. Pract. 2003; 6: 377–400.

Eissa AE, Abdelsalam M, Tharwat N, Zaki M. Detection of Saprolegnia parasitica in eggs of angelfish Pterophyllum scalare (Cuvier-Valenciennes) with a history of decreased hatchability. Int. J. Vet. Sci. Med. 2013; 1: 7–14.

Urquhart K, Collins C, Monte M, Sokolowska J, Secombes C, Collet B. Individual measurement of gene expression in blood cells from Rainbow trout On- corhynchus mykiss (Walbaum). JEAAS. 2016; 2(1):1–9.

Chauhan R. Studies on conidial fungi isolated from some fresh water fishes. Interna-tional journal of Advanced Life Sciences (IJALS), 2013; 6 (4):131–5.

Innes WT. Exotic Aquarium Fishes, 19th edition. Aquarium Incorporated, New Jersey 1966; 1(12):24-25,29-30 and530–3

McVicar AH. Ichthyophonus infections of fish. In: Roberts RJ (ed) Microbial diseases of fish. Academic Press. London, 1982: 243–69.

Austin B, Austin DA. Bacterial fish pathogens; diseases of farmed and wild fish. Springer, New York, London). 2012.

Taha M. Medical mycology “Atlas of medically important &

dermatomycosis”. 1st ed., Mecca printing house, Cairo, Egypt. 2011.

Feingold SM, Baron EJ. Bailey and Scoll's Diagnostic Microbiology. The Cv. Mos-by co., St .Louis, 1986.

Ellis MB. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute: Kew Surrey, UK, 1971.

Refai M. Isolation and Identification of fungi. Fac. Vet. Med. Cairo University. 1987

Diab AMA. Studies on the mycological affections in cultured fresh water fishes in KafrelSheikh governorate M.V.Sc Thesis (Fish diseases and management), Fac Vet. Med., Tan-ta University; 2006.

Domsch KH, Gams W, Anderson TH. Compendium of soil fungi. 2nd taxonomically revised edition by W. Gams. IHW, Eching. 2007.

Terrence CD. A practical approach to identification of yeast like organisms. Amer. Jour Microbiol 1971; 35(5):580–90.

Martin MV. Germ tube formation by oral strains of Candida albicans. J. Med. Microbiol. 1979; 12:187-193.

Cruickshank R, Duguid JP, Mariom BP, Swain RHA. Medical Microbiology the Practice of Medical Microbiology. 12th Edn., Churchill Livingstone, London, New York. 1975.

Bancroft JD, Gamble M. Techniques. 5th edition; Churchill Livingstone, London, UK., 2007: 125–38.

Marzouk MS, Rezeka SM, El Gamal MH. Some mycological investigations on cultured tilapia in Kafr El Sheikh Governorate. Kafr El Sheikh Vet. Med. J., 2003; 1: 97–111.

El-Atta MEA. Saprolegniosis in freshwa-ter cultured Tilapia nilotica (Oreochromis nilot-icus) and trial for control by using Bafry D50/500. Proceedings of the 8th International Symposium on Tilapia in Aquaculture, October 12-14, 2008, Cairo, Egypt, 2008: 1403–18

Bruno DW, Wood BP. Saprolegnia and other Oomycetes. In: Fish Disease and Disorder, Viral, Bacterial and Fungal Infections, Woo, P.T.K. and D.W. Bruno (Eds.)., CABI Publish-ing, Wallingford, Oxon, United Kingdom, 1994: 599–659.

Hussein MM, Hatai K. Saprolegnia salmonis sp. nov. isolated from sockeye salm-on, Onchrhynchus nerka. Mycoscience, 1999; 40: 387-–91.

El-Ashram AMM, Abd El-Rhman AM, Sakr SF. A contribution to saprolegniosis in cultured Nile tilapia (Oreochromis niloticus) with special reference to its control. Egypt J. Aquat. Biol. & fish. 2007; 11(3):943–55.

Chauhan R. Studies on some fresh water fishes found infected with dermatomycoses, collected from different water bodies in and around Bhopal, India. Indo Am. J. Pharm. Res. 2014; 4: 1591–6.

Ganguly S, Wakchaure R, Praveen PK, Para PA. Fungal infections in fishes: A brief review. Int. J. Pharm. Life Sci., 2016; 7: 5245–6.

Ali ShELM. Studies on the causative agents of mycotic diseases in fresh water fishes. M.V.Sc Thesis (Microbiology), Fac Vet. Med., Cairo University. 2007.

Abbas MS, Khalaf JM, Yassein SN. Iso-lation and identification of systemic mycologi-cal isolates from fishes samples that obtained from local markets in Baghdad, Iraq. World J Exp Biosci 2016; 4: 143–6.

Tartor Y, Taha M, Mahboub H, El Ghamery M. Yeast species associated with dis-eased fish: Occurrence, identification, experi-mental challenges and antifungal susceptibility testing. Aquac 2018;

Diab AMA. Studies on some systemic mycotic affection in cultured fresh water fish Ph.D Thesis (Fish diseases and hygiene), Fac Vet. Med. Alexandria university.2010.



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