HEMATO-BIOCHEMICAL AND HISTOPATHOLOGICAL CHANGES IN MUD LOACH, Misgurnus mizolepis EXPERIMENTALLY INFECTED WITH Aeromonas sobria

Jinha Yu, Junjo Han, Sung Woo Park, Daehyun Kim

Abstract


Hemato-biochemical and histopathological alterations in mud loach (Misgurnus mizolepis) infected with Aeromonas sobria were characterized in the present study. Fish infected with 1 × 106 colony forming units/mL of A. sobria (25 fish × 3 replicates × 3 tanks) and uninfected (control) fish (25 fish × 3 replicates × 3 tanks) were kept in laboratory tanks with adequate water parameters, and euthanized at 1, 3, and 5 days post-infection (PI). Infected fish became lethargic and developed rapid opercular movements, pale gills, and cutaneous petechial hemorrhages during the 5-day experimental period. Hemato-biochemical parameters, namely hematocrit, hemoglobin, total protein, total calcium, alkaline phosphatase (ALP) activity, and glucose level were significantly reduced, whereas urea (UA) level, and aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) activities were markedly increased in infected fish compared to control fish. Total leukocyte count was higher of infected than in control fish by day 3 PI, but dramatically decreased on day 5 PI. The leukogram showed the predominance of lymphocytes and neutrophils during the experimental period. Histologically, kidney and spleen showed extensive hemorrhage with cellular necrosis. Liver cells showed severely necrotic foci with karyopyknosis, karyorrhexis, karyolysis, and hyperchromatosis of the nuclear membrane. Thus, A. sobria infection causes hemato-biochemical changes such as anemia, hypoglycemia, hypoproteinemia, hypocalcemia, increased AST, ALT, and LDH activities, increased UA concentrations, and reduced ALP activity, and lead to compromised disease resistance, tissue damage, and low survival rates.

Key words: Aeromonas sobria; hematology; histopathology; Misgurnus mizolepis

 

HEMATOLOŠKO-BIOKEMIČNE IN HISTOPATOLOŠKE SPREMEMBE PRI ČINKLJAH (Misgurnus mizolepis), EKSPERIMENTALNO OKUŽENIH Z Aeromonas sobria

Povzetek: V raziskavi so bile preuÄevane hematoloÅ¡ko-biokemiÄne in histopatoloÅ¡ke spremembe pri Äinkljah (Misgurnus mizolepis), okuženih z Aeromonas sobria. Ribe, okužene z 1×106 kolonij/ml A. sobria (25 rib x 3 ponovitve v 3 bazenih) in neokužene (kontrolne) ribe (25 rib x 3 ponovitve v 3 bazenih) so bile nastanjene v laboratorijskih akvarijih z ustreznimi parametri vode in bile evtanazirane 1., 3. in 5. dan po okužbi (PI). V petdnevnem poskusnem obdobju so okužene ribe postale letargiÄne, Å¡kržne poklopce so hitro odpirale in zapirale, njihove Å¡krge so postale blede in opazne so bile podkožne petehialne krvavitve. HematoloÅ¡ko-biokemiÄni parametri, in sicer hematokrit, hemoglobin, celokupna koliÄina beljakovin, celokupni kalcij, aktivnost alkalne fosfataze (ALP) in nivo glukoze so bili znatno zmanjÅ¡ani, medtem ko so bili seÄnina (UA), aspartat aminotransferaza (AST), alanin-aminotransferaza (ALT) in laktat dehidrogenaza (LDH) v okuženih ribah opazno poveÄani v primerjavi s kontrolno skupino rib. Skupno Å¡tevilo levkocitov je bilo veÄje pri okuženih kot pri kontrolnih ribah po 3. dnevu PI, vendar se je 5. dan PI dramatiÄno zmanjÅ¡alo. V beli krvni sliki so med obdobjem poskusa prevladovali limfociti in nevtrofilci. HistoloÅ¡ka slika ledvic in vranice je pokazala obsežne krvavitve s celiÄno nekrozo. V hepatocitih so bila vidna moÄno nekrotiÄna žariÅ¡Äa s kariopiknozo, karioheksijo, kariolizo in hiperhromatozo jedrne membrane. Okužba z A. sobria je povzroÄila hematoloÅ¡ko-biokemiÄne spremembe, kot so anemija, hipoglikemija, hipoproteinemija, hipokalcemija, poveÄane aktivnosti AST, ALT in LDH, poveÄane koncentracije UA in zmanjÅ¡ana aktivnost ALP, kar je privedlo do zmanjÅ¡ane odpornosti na bolezen, poÅ¡kodbe tkiva in nizke stopnje preživetja.

KljuÄne besede: Aeromonas sobria; hematologija; histopatologija; Misgurnus mizolepis


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DOI: https://doi.org/10.26873/SVR-476-2018

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