Chinedum U. Emelike, Ugochukwu S.B. Anyaehie, Eghosa E. Iyare, Chiemeziem A. Obike, Chinedum Eleazu, Chukwuma Chukwu


We studied the acute and sub-acute toxicity of Combretum dolichopetalum leaves in experimental mice and rats respectively using standard techniques. The LD50 of the methanol extract of Combretum dolichopetalum leaves as carried out in experimental mice was obtained as more than 5000 mg/kg body weight. Administration of graded doses (100, 200, 400 and 800 mg/kg) of the extract for 21 days resulted in increases in body weights, white blood cells (WBC), Neutrophils, red blood cells (RBC), packed cell volume (PCV), haemoglobin (HGB), mean corpuscular volume (MCV) and mean cell haemoglobin (MCH) of the rats; but did not affect (P>0.05) their monocytes, mean cell haemoglobin concentration (MCHC), platelet (PLT) levels. All doses of the extract did not affect (P>0.05) the sodium, potassium, chloride, bicarbonate, urea, creatinine, total and conjugated bilirubin, alanine and aspartate amino transaminase, aspartate amino transaminase, alkaline phosphatase activities; relative liver and kidney weights of the rats, a finding that was corroborated by histology of the liver and the kidney. The extract at 100 mg/kg had no effect on the PCV and HB of the rats. The study suggested the therapeutic potentials of Combretum dolichopetalum as a blood booster. Finally, the study revealed the safety in the usage of Combretum dolichopetalum leaves in Nigerian ethnomedicine.

Key words: ethnopharmacology; Combretum dolichopetalum; toxicology; herbal medicine; nutraceutical; pharmacotherapy


Povzetek: S standardnimi metodami smo pri poskusnih miših in podganah proučevali akutno in subakutno toksičnost listov Combretum dolichopetalum. LD50 metanolnega izvlečka listov Combretum dolichopetalum je bil pri poskusnih miših nad 5000  mg/kg telesne teže. Enaindvajsetdnevno dodajanje naraščajočih odmerkov (100, 200, 400 in 800 mg/kg) izvlečka je pri poskusnih podganah povzročilo povečanje telesne mase, števila belih krvničk (WBC), nevtrofilcev, rdečih krvničk (RBC), volumna stisnjenih  eritrocitov (PCV), hemoglobina (HGB), povprečnega volumna eritrocitov (MCV) in povprečno vsebino hemoglobina v eritrocitih (MCH), ni pa vplivalo (p > 0,05) na število monocitov, povprečno koncentracijo hemoglobina v volumnu eritrocitov (MCHC)  ter na povprečno vrednost trombocitov (PLT). Nobeden od odmerkov izvlečka ni vplival na (p > 0,05) vrednosti natrija, kalija, klorida, bikarbonata, sečnine, kreatinina, skupnega bilirubina in vezanega bilirubina, alanina, aspartatne amino transaminaze, aspartatne amino transaminaze, alkalne fosfataze; relativno težo jeter in ledvic podgan, kar je bilo v skladu s histološko preiskavo jeter in  ledvic. Izvleček v odmerku 100 mg/kg ni vplival na PCV in HB podgan. Študija tako kaže na možnost uporabe rastline Combretum  dolichopetalum za izboljšanje krvne slike. Raziskava je dokazala varnost uporabe listov Combretum dolichopetalum, ki se tradicionalno uporabljajo v Nigeriji v etnomedicini.

Ključne besede: etnofarmakologija; Combretum dolichopetalum; toksikologija; zeliščna zdravila; hranila; farmakoterapija

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(1.) El Kabbaoui M, Chda A, El-Akhal J, et al. Acute and sub-chronic toxicity studies of the aqueous extract from leaves of Cistus ladaniferus L. in mice and rats. J Ethnopharmacol 2017; 209: 147–56.

(2.) Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules 2016; 21: 559.

(3.) Han JS, Lee BS, Han SR, et al. A subchronic toxicity study of Radix Dipsaci water extract by oral administration in F344 rats. Regul Toxicol Pharmacol 2016; 81: 136–45.

(4.) Yuet Ping K, Darah I, Chen Y, Sreeramanan S, Sasidharan S. Acute and subchronic toxicity study of Euphorbia hirta L. methanol extract in rats. BioMed Res Int 2013; 2013: e182064. doi: 10.1155/2013/182064

(5.) Ezeja MI, Anaga AO, Asuzu IU. Acute and sub-chronic toxicity profile of methanol leaf extract of Gouania longipetala in rats. J Ethnopharmacol 2014; 151: 1155–64.

(6.) Uzor PF, Osadebe PO, Omeje EO, Agbo MO. Bioassay guided isolation and evaluation of the antidiabetic principles of Combretum dolichopetalum root. Br J Pharm Res 2014; 4: 2155–71.

(7.) Burkhill HM. The useful plants of West Tropical Africa. 2nd ed. Vol. 1, Families A–D. Kew : Royal Botanic Gardens, 1985: 960.

(8.) Asuzu IU, Onu OU. Anti-ulcer activity of the ethanolic extract of Combretum dolichopetalum roots. Int J Crude Drug Res 1990; 28: 27–32.

(9.) Udem SC, Madubunyi I, Okoye JO, Anika SM. Anti-hepatotoxic effects of the ethanolic extracts of Combretum dolichopetalum root bark and Morinda lucida leaf. Fitoterapia 1997; 68: 21–4.

(10.) Barku VYA, Opoku-Boahen, Dali, G. Ethnobotanical study of wound healing plants in kpando traditional area, Ghana. Int J Phytomed 2014; 6: 564–72.

(11.) Ameyaw Y, Barku VYA, Ayivor J, Forson A. Phytochemical screening of some indigenous medicinal plant species used in the management of diabetes mellitus in Ghana. J Med Plants Res 2012; 6: 4573–81.

(12.) Onoja SO, Udeh NE. Antidiarrheal effects of hydromethanolic extract of Combretum dolichopetalum leaves in mice. J Coastal Life Med 2015; 3: 910–3.

(13.) Asuzu IU, Njoku JC, Njoku J. The pharmacological properties of the ethanolic root extract of Combretum dolichopetalum. Phytother Res 1992; 6: 125–8.

(14.) Asuzu IU, Adimorah RI. The anti-inflammatory activity of extracts from the root of Combretum dolichopetalum. Phytomedicine 1998; 5: 25–8.

(15.) National Research Council, NRC. Guide for the care and use of laboratory animals. Bethesda : National Institute of Health, 1985.

(16.) OECD. Guidelines for testing of chemicals no. 423. Acute oral toxicity – acute toxic class method. Paris : Organisation for Economic Co-Operation and Development, OECD, 2001.

(17.) Enegide C, David A, Fidelis SA. A new method for determining acute toxicity in animal models. Toxicol Int 2013; 20: 224–6.

(18.) Akomas SC, Ijioma SN, Emelike CU. In vivo and in vitro activity of ficussur ethanolic extract on the gastrointestinal tract. Br Biotechnol J 2014; 4: 1182–90.

(19.) OECD. Guidelines for testing of chemicals no. 407. Repeated dose 28-days oral toxicity study in rodents. Paris : Organisation for Economic Co-Operation and Development, OECD, 1995.

(20.) Marcela CA, Neila MS, Paula MV, et al. Acute and sub chronic toxicity study of aqueous extract from the leaves and branches of Campomanesia velutina (Cambess) O. Berg. J Ethnopharmacol 2017; 201: 17–25.

(21.) Eleazu CO, Okafor PN. Use of unripe plantain (Musa paradisiaca) in the management of diabetes and hepatic dysfunction in streptozotocin induced diabetes in rats. Interv Med Appl Sci 2015; 7: 9–16.

(22.) Kalu W, Okafor P, Ijeh I, Eleazu C. Effect of kolaviron, a biflavanoid complex from Garcinia kola on some biochemical parameters in experimentally induced benign prostatic hyperplasic rats. Biomed Pharmacother 2016; 83:1436–43.

(23.) Clarke EGC, Clarke ML. Veterinary toxicology. London : Casell and Collier Macmillan Publishers, 1977: 268–77.

(24.) OECD. Harmonised integrated classification system for human health and environmental hazards of chemical substances and mixtures. Paris : OECD, 2001: Chapter 2.1. (adopted 14th August 2001) (OECD Series on testing and assessment, No. 33.)

(25.) Lee MY, Shin IS, Seo CS, Kim JH, Han SR, Shi HK. Sub chronic oral toxicity studies of the traditional herbal formula Bangpunglongseong-an in Crl:CD (SD) rats. J Ethnopharmacol 2012; 144: 720–5.

(26.) Thanabhorn S, Jaijoy K, Thamaree S, Ingkaninan K, Panthong A. Acute and subacute toxicity study of the ethanol extract from Locinera japonica Thunb. J Ethnopharmacol 2006; 107: 370–3.

(27.) Khan TA, Zafar F. Haematological study in response to varying doses of estrogen in broiler chicken. Int J Sci 2005; 4: 748–51.

(28.) Liju VB, Jeena K, Kuttan R. Acute and subchronic toxicity as well as mutagenic evaluation of essential oil from turmeric (Curcuma longa L). Food Chem Toxicol 2013; 53: 52–61.

(29.) Sunmonu TO, Oloyedem OB. Performance and haematological indices in rats exposed to monocrotophos contamination. Hum Exp Toxicol 2010; 29: 845–50.

(30.) Ugwuezumba PC, Nwankpa P, Emengaha FC, et al. Alteration of haematological indices on administration of ethanol leaf and root extracts of sida acuta in albino wistar rats. Asian J Sci Technol 2018; 9: 8156–9.

(31.) Olson H, Betton G, Thomas D, et al. Concordance of the toxicity of pharmaceuticals in humans and in animals. Regul Toxicol Pharmacol 2000; 32: 56–67.

(32.) Dekant W, Vamvakas S. Biotransformation and membrane transport in nephrotoxicity. Crit Rev Toxicol 1996; 26: 309–34.

(33.) Akindele AJ, Adeneye AA, Salau OS, Sofidiya MO, Benebo AS. Dose and timedependent sub-chronic toxicity study of hydroethanolic leaf extract of Flabellaria paniculata Cav. (Malpighiaceae) in rodents. Front Pharmacol 2014; 5: e78. doi: 10.3389/fphar.2014.00078

(34.) Gowda S, Desai PB, Kulkarni SS, Hull VV, Math AAK, Vernekar SN. Markers of renal function tests. N Am J Med Sci 2010; 2: 170–3.

(35.) Nayak SB. Maniple manuel of clinical biochemistry. New Delhi : Jaypee Brothers, Medical Publishers, 2007: 300.

(36.) Achi N, Ohaeri C, Ijeh I, Eleazu C, Igwe K, Onyeabo C. Ameliorative potentials of methanol fractions of Cnidoscolus aconitifolius on some hematological and biochemical parameters in streptozotocin diabetic rats. Endocr Metab Immune Disord Drug Targ 2018; 18(6): 637–45.

(37.) Eleazu CO, Okafor PN, Ijeh I. Biochemical basis of the use of cocoyam (Colocassia esculenta L.) in the dietary management of diabetes and its complications in streptozotocin induced diabetes in rats. Asian Pac J Trop Dis 2014; 4: S705–S711.

(38.) Balogun SO, Da Silva IF, Colodel EM, De Oliveira RG, Ascêncio SD, Martins DO. Toxicological evaluation of hydroethanolic extract of Helicteres sacarolha A. J Ethnopharmacol 2014; 157: 285–91.

(39.) Ying P, Yunen L, Xiaodong X. Change trends of organ weight background data in Sprague Dawley rats at different ages. J Toxicol Pathol 2013; 26: 29–34.



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