Sawsan M. El-Sheikh, Mohamed H. Khairy, Naglaa Z.H. Eleiwa, Osama E. Abdalla, Asmaa G. Abd El-Monsef


This study aimed to investigate the efficacy of Sanguinarine phytobiotic, Sodium butyrate compared to ampicillin in treatment of Clostridium perfringens infection in broiler chickens with a special reference to their effects on growth performance, hematobiochemical and immunological profile.  A total of 150 one-day old Cobb broiler chicks were used in this study. On day 14th of age all chicks were divided into 5 equal groups (30 each). Group (G1) non-infected, non-treated (control), G2 infected with C. perfringens, non treated, G3 infected, treated with Sodium butyrate, G4 infected and treated with Sanguinarine phytobiotic and G5 infected and treated with ampicillin. Administration of drugs in drinking water was continued for 5 days from 18-23 day of age. The results revealed that the infected broiler chickens with C. perfringens and non treated(G2) showed clinical signs of necrotic enteritis represented by loss of appetite, diarrhea, dehydration, anorexia in addition to a recorded mortality rate 30% and lesion score 80%. Moreover, significant reduction (P<0.05) in body weight, weight gain, erythrogram, phagocytosis and phagocytic index, Nitric oxide, HI titers, total protein, albumin and total globulin beside significant increase  (P<0.05) in feed conversion rate (FCR), leukogram, liver enzymes, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase (AST-ALT and ALP), uric acid and creatinine associated with non-significant increase  (P<0.05) in globulin fractions were observed. Treatment of the infected chickens with these drugs led to improvement in clinical signs, mortality rate, lesion score, growth performance, hematobiochemical and immunological profile compared with infected non treated birds (G2), but ampicillin had superior effect against Costridium perfringes infection. It could be concluded that C. perfringens infection in broiler chickens induced adverse effects on growth performance, hemato-biochemical and immumological profile of birds which could be reversed or ameliorated by using Sanguinarine phytobiotic, Sodium butyrate or ampicillin. So,  the study recommended the use of such treatments hand in hand with antibiotics for controlling necrotic enteritis in broilers.

Key words: necrotic enteritis; Sodium butyrate; phytobiotic; ampicillin

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