POSSIBLE ANALGESIC, 1ANTI-INFLAMMATORY AND ANTI-ULCEROGENIC EFFECTS OF Alhagi maurorum METHANOLIC EXTRACT IN RATS AND MICE

Hesham A. Khalifa, Asmaa M. Elattar, Naglaa Z.H. Eleiwa

Abstract


Alhagi maruroum, a species of family leguminosae, has high contents of flavonoids, triterpenes, sterols and glycosides. It is a woody perennial herb that was used in the folk medicine for gastric disturbance, rheumatic pains, liver disease and urinary tract infection. This study was designed to spot the light on some pharmacological activities of Alhagi maurorum. The fresh aerial parts of the plant were washed, dried at room temperature, crushed into fine powder, extracted with 80% methanol for three days with frequent agitation, filtrated and dried by rotatory evaporator under reduced pressure at 45°C. The concentrated filtrate then was lyophilized in lyophilizer. The extract was tested for some pharmacological activities (analgesic, anti-inflammatory and antiulcerogenic) in rats and mice. The current results proved that administration of Alhagi maurorum aqueous methanolic extract in mice at dose of 200 and 400 mg/kg BW exhibited analgesic effect that was noticed from a significant elevation in the reaction time of mice in a dose dependent manner when compared to the control mice by using hot plate method. Moreover, Alhagi maurorum aqueous methanolic extract demonstrated anti-inflammatory effect by using hind paw edema method in rats as confirmed by a significant dose dependent reduction in the thickness of the hind paw edema compared with the control group. Additionally, administration of Alhagi extract elicited a significant improvement in gastric damage induced by indomethacin in rats where mean ulcer score in alhagi 200 and alhagi 400 groups was 1.25±0.045 and 0.25±0.004, respectively compared with 1.75±0.054 of indomethacin group. It could be deduced from those results that Alhagi maurorum aqueous methanolic extract owns analgesic, anti-inflammatory, and antiulcerogenic activities.

Key words: Alhagi maurorum; aqueous methanolic extract; analgesic; anti-inflammatory; anti-ulcerogenic


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Biswas A, Bari MA, Roy M, Bhadra SK. Inherited folk pharmaceutical knowledge of tribal people in the Chittagong Hill Tracts, Bangladesh. Indian J Tradit knowl 2010; 9: 77–89.

Revathi P, Parimelazhghan T. Traditional Knowledge on medicinal plants used by Irula tribe of Hasanur hills, Erode District ,Tamil Nadu, India. Ethnobot Leaflets 2010; 2010: 136–60.

Dhoniya S, Parihar SK. Isolation and identification of flavonoids from Alhagi maurorum. J pharmacog phytochem 2018; 7: 1321–5.

Bolus L. Medicinal Plants of North Africa. Cairo, Egypt, Reference Publications Inc. 1983: 368.

Ghosal S, Srivastara RS, Battacharya SK, Debanath PK. The active principles of Alhagi pseuddalhagi Bphenethylamine and tetrahydro isoquinoline bases. Planta Med 1974; 26: 318–26.

Bakht J, Gohar N, Shafi M. In vitro anti-bacterial and antifungal activity of different solvent extracted samples of Alhagi maurorum. Pak. J. Pharmacet. Sci. 2014 Nov 1; 27 (27): 1955–61

Al-Yahya MA, Mossa JS, Al-Badr AA, Tariq MS, Al-Meshal IA. Phytochemical and biological studies on Saudi medicinal plants Part 12. A study on Saudiplants of family Leguminosae. Int J Crud Drug Res 1987; 25: 65–71.

El-Saayed NH, Inshak MS, Kandil FI, Mabry TJ. Flavonoids of Alhagi graecolum. Pharmazie 1993; 48: 68–89.

Singh Y, Wadhwani AM, Johri BM. Dictionary of Economic Plants of India. New Delhi, India: Indian Council of Agricultural Research, 1990.

Kayani SA, Masood AY, Achakzai AK, Anbreen S. Distribution of secondary metabolites in plants of Quetta-Balochistan. Pak J Bot. 2007; 39 (4): 1173.

Ahmad N, Bibi Y, Raza I, Zahara K, Khalid N, Bashir T, Tabassum S. Traditional uses and pharmacological properties of Alhagi maurorum: A review. Asian Pac J Trop Dis. 2015; (11): 856–61.

Ahmad N, Shinwari, ZK, Hussain J, Perveen R. Phytochemicals, antibacterial and antioxidative investigations of Alhagi maurorum medik. Pak J Bot 2015; 47: 121–4.

Varshochi F, Asadollahi K. Alhagi; traditional and modern medicine effective against kidney stones. J Nephropharmacol 2017; 6: 15-16.

Singh Y, Wadhwani AM, Johri BM. Dictionary of Economic Plants of India. New Delhi, India: Indian Council of Agricultural Research, 1990.

Chakravarty A. The Plant Wealth of Iraq (A Dictionary of Economic Plants). Botany Directorate, Ministry of Agriculture & Agrarian Reform, England Department of Botany, University of Karachi1976; 100: 226–30.

Encyclopedia of medicinal plants in UAE.Health Authority Abu Dhabi.Zaied Center for Traditional Medicine and Herbs Researches 2005, 15-20.

Naseri MKG, Mard SA. Gastroprotective effect of Alhagi maurorum on experimental gastric ulcer in rats. Pak J Med Sci 2007; 23: 570–3.

Singh S, Mukerjee A, Mishra SB, Srivastava Y. Assessment of anti-arthritic potential of ethanolic extract and its fractions of aerial parts of Alhagi pseudalhagi (Bieb.) Desv. in animal models. Oriental Pharmacy and Experimental Medicine. 2018 1; 18 (3): 199–208

Shaker E, Mahmoud H, Mnaa S. Anti-inflammatory and anti-ulcer activity of the extract from Alhagi maurorum (camelthorn). Food Chem Toxicol 2010; 48: 2785–90.

Awaad AAS, Maitland DJ, Soliman GA. Antiulcerogenic activity of Alhagi maurorum. Pharma Biol 2006; 44: 292-296.

Srinivasan D, Sangeetha N, Suresh T, Perumalsamy PL. Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharmacol 2001; 74: 217–20.

Turner, R.A. Screening Method in pharmacology Academic Press Inc, London, p. 1965; 104-157.

AL-Rahawi K, AL-Kaf A, Yassin S, EL-Nabtity S, EL-Sayed K . Synthesis and Biological Activities of2-Carboxyphenyloxamoylamino acids ,their salts with 2- ethyl-6,9-Diaminoacridine and D-glucosamine. Adv Pharmacoepidem Drug Safety 2013; 2: 129–35.

Atta AH, Mouneir SM. Antidiarrhoeal activity of some Egyptian medicinal plant extracts. J Ethnopharmacol, 2004; 92: 303–9.

Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for antiinflammatory drugs. Proceedings of the society for experimental biology and medicine. 1962; 111 (3): 544–7.

Wilhelmi G, Menasse-Gdynia R. Gastric mucosal damage induced by non-steroid anti-inflammatory agents in rats of different ages. Pharmacology. 1972; 8 (4-6): 321–8.

Eleiwa NZ, Bakr RO, Mohammed SA. Phytochemical and pharmacological screening of seeds and fruits pulp of Cucurbita moschata duchesne cultivated in Egypt. International Journal of Pharmacognosy and Phytochemistry. 2014; 29 (1): 2051².

Abdul Hafeez L, Shahabuddin M, Aisha N, Khalid M. Alhagi maurorum A convenient source of lupeol. Industrial crops and products 2011; 34: 1141–5.

Ghannadi A, Hajhshemi V, Jafarabadi H. investigation of the analgesic and anti- inflammatory effects of nigella Sativa seed polyphenols. J Med Food 2005; 8: 488–93.

Banerjee SA, Mukherjee AN, Chatterjee TK. Evaluation of analgesic activities of methanolic extract of medicinal plant Juniperus communis Linn. Int J Pharm Pharmaceutical Sci 2012; 4:

–50.

Schinella GR, Tournier HA, Prieto JM, Mordujovich D, Rois JL. Antioxidant activity of anti-inflammatory plant extracts. Life Sci 2002; 70: 1023–33.

Awaad AS, El-Meligy RM, Qenawy SA, Atta AH, Soliman GA. Anti-inflammatory, antinociceptive and antipyretic effects of some desert plants. Journal of Saudi Chemical Society. 2011; 15 (4): 367–73

Lagharia AH, Memona S, Nelofar A, Khanc KM. Alhagi maurorum A convenient source of lupeol. Indust Crops Prod 2011; 34: 1141–5.

Menassé R, Hedwall PR, Kraetz J, Pericin C, Riesterer L, Sallmann A. Pharmacological properties of diclofenac sodium and its metabolites. Scand J Rheumatol Suppl 1978; 22: 5–16.

Sulaiman GM. Antimicrobial and cytotoxic activities of methanol extract of Alhagi maurorum. African J Microbiol Res 2013; 7: 1548–57.

Sairam K, Rao ChV, Babu MD, Kumar KV, Agrawal VK, Goel RK. Antiulcerogenic effect of methanolic extract of Emblica officinalis: an expe-rimental study. J Ethnopharmacol 2002; 82: 1-9.

Parmas NS. The gastric anti-ulcer activity of naringenin, a specific histidine decarboxylase inhibitor. Int J Tissue React 1983; 5: 415–21.

Singh VP, Yadav B, Pandey VB. Flavanone glycosides from Alhagi pseudoalhagi. Phytoche-mistry 1999; 51: 587–90

Atta AH, El-Sooud KA. The antinociceptive effect of some Egyptian medicinal plant extracts. J Ethnopharmacol 2004; 95: 235-8.

Matsunaga T, Hasegawa C, Kawasuji T, Suzuki H, Saito H, Sagioka T. Isolation of the antiulcer compound inessential oil from the leaves

of Cryptomeria japonica. Biol Pharm Bull 2000; 23: 595–8.

Hiruma-Lima CA, Gracioso JS, Toma W, Almeida AB, Paula ACB, Brasil DSB. Gastro-protective effect of aparisthman, a diterpene isolated from Aparisthmium cordatum, on experimental gastric ulcer models in ratsand mice. Phytomedicine 2001; 8: 94–100.

Sertié JAA, Carvalho JCT, Panizza S. Antiulcer activity of the crude extract from the leaves of Casearia sylvestris. Pharmaceutical Biol 2000; 38: 112–9.


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