Alaa Eldin M.A. Morshdy, Mohamed A. Hussein, Ahmed E. Tharwat, Basma A. Fakhry


Due to recent spread of multiple drug resistant pathogens, this study was peformed to investigate the presence of multi-drug resistant enterotoxigenic Staphylococcus aureus (S. aureus) in some ready to eat meat products (RTE). For this, one hundred and forty samples of ready to eat meat sandwiches were collected from restaurants and street vendors in Zagazig city, Egypt. Staphylococcus aureus is one of the most important food poisoning bacteria in RTE. The counts were 3.31 ± 0.49, 2.86 ± 0.36, 3.28 ± 0.24, 3.92 ± 0.41, 2.52 ± 0.11, 3.64 ± 0.39 and 3.12 ± 0.35 log10 CFU/g in examined kofta, luncheon, burger, shawarma, hawawshi, liver and sausage sandwiches, respectively. The examined sandwiches were categorized into good (32.1%), acceptable (32.9%), unsatisfactory (26.4%) and potentially hazard (8.6%). About 72.7 % of examined S. aureus strains carry one or more staphylococcal enterotoxin (se) genes and mecA gene detected in 81.8% of coagulase positive S. aureus. The antibiogram showed that 100% of S. aureus isolates were resistant to kanamycin, 92% for penicillin and neomycin, 84% for oxacillin and erythromycin and 68% for ampicillin and nalidixic acid. The average of multi-antibiotic resistant (MAR) index of isolated S. aureus was 0.59. Moreover, five isolates were resistant to all tested antibiotic.

Key words: Staphylococcus aureus; methicillin resistant; enterotoxin; ready to eat


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