MORPHOLOGICAL AND HISTOLOGICAL STUDIES ON PARATHYROID GLAND OF ADULT MALE GOAT (CAPRA HIRCUS)

Nehal I.A. Goda, Shafika A. El sayed, Rasha R. Beheiry, Suzan A.A. Ismail

Abstract


The parathyroid glands are essential endocrine glands as they produce hormones that maintain calcium within the normal level in blood through secretion of parathormone. The removal of parathyroid glands leads to fatal levels of hypocalcemia. The number of parathyroid glands is species specific. The current investigation was performed on 20 healthy freshly slaughtered adult male goats. Fifteen specimens were immediately fixed in 10% buffered neutral formalin, then processed for histological and immunohistochemical examination. Other 5 specimens were handled for transmission electron microscope. The present work was conducted to study the morphological and histological characteristics of parathyroid glands of goat. Parathyroid glands comprised of two pairs, each pair formed from external and internal glands. External parathyroid gland was rounded or oval in shape. Its location was varied in the same animal where it may be located cranial to thyroid gland or beside the submandibular salivary gland. Internal parathyroid gland was embedded inside thyroid tissue. It appeared as pale rounded area at the end of cranial part of the right and left thyroid lobe. The histological findings demonstrated that, each parathyroid gland is surrounded by a thin capsule of dense irregular connective tissue. The glandular parenchyma is divided by short thick septa into ill distinct compartments. Each compartment had numerous numbers of chief cells. The active chief cells were polygonal in shape with oval nuclei. Immuno-histochemical findings revealed that they are positively reacted against chromogranin antibody. Electron microscope revealed that the cytoplasm have abundant mitochondria, rough endoplasmic reticulum, evenly distributed golgi apparatus and numerous secretory granules. On the contrary, the inactive chief cells have more vacuolated cytoplasm which contains less cell organelles.

Key words: parathyroid gland; chief cells; ultrastructure; chromogranin


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(1) Kumar S, Rama Rao CA, Kareemulla K, Venkateswarlu B. Role of goats in livelihood security of rural poor in the less favoured environments. Indian J Agric Econ 2010; 65 (4): 1–22.

(2) Sahlu T, Goetsch AL. A foresight on goat research. Small Rumin Res 2005; 60 (1-2): 7–12.

(3) Modarai B, Sawyer A, Ellis H. The glands of Owen. J R Soc Med 2004; 97 (10): 494–5.

(4) Bienzle D., Jacobs RM, Lumsden JH. Relationship of serum total calcium to serum albumen in dogs, cats, horses and cattle. Can Vet J 1993; 34 (6): 360–4.

(5) Metwally MA, Attia HF. Anatomical and Histological Studies on the Parathyroid gland of the Camel (Camelus Dromadrius). In the International Scientific Conference on Camel. Saudi Arabia 2006 May, pp: 1484–94.

(6) Ramasamy I. Recent advances in physiological calcium homeostasis. Clin Chem Lab Med 2006; 44 (3): 237–73.

(7) Ross MH, Romrell LJ, Kaye GI. Histology.3rd ed.1995 Williams and Wilkins Sydney. Tokyo. PP: 113.

(8) Al-Ramadan SY, Ali AM, Al-Zghoul MB, Althnian TA, Alzayer MA. Macro-and Micro-Morphological Studies on the Parathyroid Glands of Dromedary Camel. Pak Vet J 2016; 37: 59–64.

(9) Capen CC, De Lellis RA, Yarrington JT. Endocrine system. In: Handbook of Toxicologic Pathology, Vol 2 (Haschek WM, Rousseaux CG, Wallig MA, eds). Academic Press 2002; New York, 681–783.

(10) Chen H, Emura S, Yao XF, Shoumura S. Morphological study of the parathyroid gland and thyroid C cell in senescence-accelerated mouse (SAMP6), a murine model for senile osteoporosis. Tissue Cell 2004; 36 (6): 409–15.

(11) Emura S, Tamada A, Hayakawa D, Chen H, Shoumura S. Electron Microscopic Study of the Parathyroid Gland of Rattus rattus. Okajimas Folia Anat Jpn 1999; 76 (1): 71–80.

(12) Chen H, Senda T, Emura S, Kubo K. An update on the structure of the parathyroid gland. Open Anat J 2013; 5 (1): 1–9.

(13) Tomita T. Immunocytochemical staining patterns for parathyroid hormone and chromogranin in parathyroid hyperplasia, adenoma and carcinoma. Endocr Pathol 1999; 10 (2): 145–56.

(14) Lu M, Kjellin H, Fotouhi O, Lee L, Nilsson I, Höög A, Lehtiö J, Larsson C. Molecular Differences Between Oxyphilic Cell and Chief Cell Parathyroid Adenoma. In endocrine reviews 2014, (Vol. 35, No. 3). 2055 L ST NW, suite 600, washington, dc 20036 USA: endocrine Soc.

(15) Gonzalez Campos A. Isolation of human parathyroid cell type as a tool for investigating the mechanisms of human primary aldosteronism. Ph.D. thesis, Universita Degli Studi Di Padova, 2014.

(16) Oka T, Onoe K, Matsumiya K, Takaha M, Yoshioka T, Koide T, Sonoda T, Kimura M, Sakurai M. Light microscopical immunohistochemical study on parathyroid adenoma in primary hyperparathyroidism. Urol Int 1994; 52: 121– 5.

(17) Cohn DV, Fasciotto BH, Zhang JX, Gorr SU. The chemistry and biology of chromogranin A (secretory protein-I of the parathyroid and other endocrine glands. In: The Parathyroids (Bilezikian, J. P., Levine, M., Marcus, R., eds.), 1994 pp. 107–19. Raven Press, New York, NY.

(18) Cotesta D, Caliumi C, Alò P, Petramala L, Reale M G, Masciangelo R, Signore A, Cianci R, D’Erasmo E, and Letizia C. High plasma levels of human chromogranin A and adrenomedullin in patients with pheochromocytoma. Tumori 2005; 91 (1): 53–8.

(19) Welin S, Stridsberg M, Cunningham J, Granberg D, Skogseid B, Oberg K, Eriksson B, Janson ET. Elevated plasma chromogranin A is the first indication of recurrence in radically operated midgut carcinoid tumors. Neuroendocrinol 2009; 89 (3): 302–7.

(20) Escribano D, Soler L, Gutie´rrez AM, Martı´nez-Subiela S, Cero´n JJ. Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress. Anim 2012; 7 (4): 640–7.

(21) Colombo B, Curnis F, Foglieni C, Monno A, Arrigoni G, Corti A. Chromogranin A expression in neoplastic cells affects tumor growth and morphogenesis in mouse models. Cancer Res 2002; 62 (3): 941–6.

(22) Bancroft JD, Gamble M. Theory and practice of histological techniques. Elsevier Health Sciences; 2008.

(23) Glauert AM, Lewis PR. Biological specimen preparation for transmission electron microscopy. 1998; Volume 17, Portland press; London.

(24) Manley NR. Embryology of the Parathyroid Glands. In Hypoparathyroidism 2015; (pp. 11-18). Springer, Milano.

(25) Seethala RR, Virji MA, Ogilvie JB. Pathology of the parathyroid glands. In: Barnes L, 3rd ed. surgical pathology of the head and neck, vol. III. New York, NY: Informa Health Care 2009; 1429–72.

(26) Hussin AM, Al-taay MM. Histological study of the thyroid and parathyroid glands in Iraqi buffalo ‘bubalus bubalis’ with referring to the seasonal changes. Bas J Vet Res 2009; 8 (1): 26–38.

(27) Capen CC, Mohr U. Anatomy, histology, ultrastructure, parathyroid, rat. 2nd ed., Springer Verlag, Berlin, Heidelberg, New York, 1996; 330–33.

(28) Babu A P, Dhyana VR, Rajasekhar U, Ramayya PJ. Microanatomical studies on the parathyroid glands of horse. Intl J of Sci 2016; 5 (2): 1238–42.

(29) Wardle EN, Kurihara I, Saito T, Obara K, Shoji Y, Hirai M, Soma J, Sato H, Imai Y, Abe K, Yuasa S. Oxyphil cell function in secondary parathyroid hyperplasia. Nephron 1996; 73 (4): 580–6.

(30) Ritter CS, Haughey BH, Miller B, Brown AJ. Differential gene expression by oxyphil and chief cells of human parathyroid glands. J Clin Endocrinol Metab 2012; 97 (8): E1499–505.

(31) Cohn DV, Fasciotto BH, Reese BK, Zhang JX. Chromogranin A: a Novel Regulator of Parathyroid Gland Secretion. J Nutr 1995; 125 (suppl 7): 2015S -19S.

(32) Huttner WB, Gerdes HH, Rosa P. The granin (chromogranin/secretogranin) family. Trends Biochem Sci 1991; 16: 27–30.

(33) Schmid KW, Hittmair A, Ladurner D, Sandbichler P, Gasser R, Tiitsch M. Chromogranin A and B in parathyroid tissue of cases of primary hyperparathyroidism: an immunohistochemical study. Virchows Archiv A. 1991;418 (4): 295–9.




DOI: http://dx.doi.org/10.26873/SVR-650-2018

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