EFFECT OF NATURAL AND SYNTHETIC FOOD COLORANTS ON SPERMATOGENESIS AND THE EXPRESSION OF ITS CONTROLLING GENES

Metwally Montaser, Rasha A.E. Abiya, Mohamed Afifi, Salina Saddick, Ayed S. Allogmani, Omar A. Almaghrabi

Abstract


The use of food additives is controversial. However, data regarding their effects on fertility genes are still very sparse. The present study was designed to assess the effects of two coloring agents, carmoisine (synthetic) and curcumin (natural) on the expression of some genes with fertility impact. Sixty three male Sprague-Dawley albino rats were orally administered carmoisine and curcumin in three doses, acceptable daily intake (ADI), 5x- and 10x- ADI for 15, 30, and 45 days. Sperm analysis and testicular expression level of biomarkers Testin, Glial cell derived neurotrophic factor (GDNF), tyrosine kinase receptor (c-KIT), follicle stimulating hormone receptor (FSHR), A kinase anchor protein 3 (PRKA3), spermatogenesis associated 7 (Spata7), Stage-specific embryonic antigen-1 (SSEA1) genes were measured and supported with histopathological studies on rat testes tissues. The recorded results revealed significant down regulation of the tested genes in rats supplemented with carmoisine in time and dose dependent manner. However, these declines were also observed after treatment with medium and high doses of curcumin. Sperm counts were significantly decreased after carmiosine treatment in a dose dependent manner, it was 74.6±6.36, 74.00±6.63, 49.00±0.28 and 147.00±3.2 for ADI, 5xADI, 10xADI and control group respectively, without any changes after curcumin treatment. Also, histopathological studies indicated deleterious effect with medium and high doses of carmiosine. In Conclusion, carmoisine induced hazardous effects on fertility at different levels when consumed in concentrations higher than the acceptable daily-authorized level (50 mg/kg b.wt). However, curcumin as a natural food color is saver than carmoisine up to certain levels.

Key words: carmoisine; curcumin; spermatogenesis genes; rat testis


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References


(1) Kumar M, Venkatesh M, Pramod K. Colourants and Additives: Existing and Emerging Safety Concerns. Int J Pharm Clin Res 2017; 9: 525–33.

(2) Scotter MJ. Emerging and persistent issues with artificial food colours: natural colour additives as alternatives to synthetic colours in food and drink. Quality Assurance and Safety of Crops & Foods 2011; 3: 28–39.

(3) Kadasa NM, Abdallah H, Afifi M, Gowayed S..Hepatoprotective Effects of Curcumin Against Diethyl Nitrosamine Induced Hepatotoxicity in Albino Rats. Asian Pac J Cancer Prev 2014; 16: 103–8.

(4) Alisi IO, Uzairu A, Abechi S, Idris S. Evaluation of the antioxidant properties of curcumin derivatives by genetic function algorithm. J. Adv. Res. 2018; 12; 47–54.

(5) Tvrdá E, Lukáč N, Jambor T, Lukáčová J, Massányi P. Curcumin in male fertility: effects on spermatozoa vitality and oxidative balance. J Microbiol Biotech Food Sci 2015; 4: 120–4.

(6) Zhang L, Diao RY, Duan YJ, Yi TH, Cai Z. In vitro antioxidant effect of curcumin on human sperm quality in leucocytospermia.Andrologia 2017; 49: e12760.

(7) Lonare M, Kumar M, Raut S, More A, Doltade S, Badgujar P, Telang A. Evaluation of Ameliorative Effect of Curcumin on Imidacloprid-Induced Male Reproductive Toxicity in Wistar Rats. Environ.Toxicol 2016; 31(10): 1250–63.

(8) Amin KA, Abdel-Hameid H, AbdElsattarA H. Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food ChemToxicol2010; 48: 2994–9.

(9) Montaser M, Alkafafy M. Effects of Syn-thetic Food Color (Carmoisine) on Expression of Some Fuel Metabolism Genes in Liver of Male Alb-ino Rats. Life Science Journal 2013; 10: 2191–8.

(10) Khayyat L, Essawy A, Sorour J, Soffar A. Tartrazine induces structural and functional aberrations and genotoxic effects in vivo. PeerJ 2017; 5: e3041.

(11) Mehedi N, Ainad-Tabet S, Mokrane N, Addou S, Zaoui C, Kheroua O., Saidi D. Reproductive Toxicology of Tartrazine (FD and C Yellow No. 5) in Swiss Albino Mice.Am J PharmacolToxicol 2009; 4: 130–5.

(12) Fijer A, Al-Mashhedy L. Influence of Carmosine (E122) on oxidative stress status and the protective effect of vitamins C and E in male rats. Int J Pharmtech Res 2016; 9: 261–70.

(13) Bancroft JD, Gamble M. Theory and Practice of Histological Technique. 5th Ed., Churchill, Livingston, Edinbergh ,London and New York 2018: 273: 292.

(14) Morakinyo AO, Iranloye BO, Adegoke OA. Anti reproductive effect of calcium channel blockers on male rats .Reprod. Med. Biol 2009; 8: 97–102.

(15) Visweswaran B, Krishnamoorthy G. Oxidative Stress by Tartrazine in the Testis of Wistar Rats. IJ Pharm BiolSci 2012; 2: 44–9.

(16) Ghonimi W, Elbaz A. Histological Changes of Selected Westar Rat Tissues Following the Ingestion of Tartrazine With Special Emphasis on the Protective Effect of Royal Jelly and Cod Liver oil. J CytolHistol 2015; 6 (4): 1–6.

(17) Sudjarwo SA, Sudjarwo G, Koerniasari K. Protective effect of curcumin on lead acetate-induced testicular toxicity in Wistar rats. Res Pharm Sci2017; 12: 381–90.

(18) Boussada M, Lamine J, Bini I, Abidi N, Lasrem M, El-Fazaa S, El-Golli N. Assessment of a sub-chronic consumption of tartrazine (E102) on sperm and oxidative stress features in Wistar rat. Int Food Res J2017; 24: 1473–81.

(19) Grima J, Zhu L, Cheng CY. Testin Is Tightly Associated with Testicular Cell Membrane upon Its Secretion by Sertoli Cells whose Steady-state mRNA Level in the Testis Correlates with the Turnover and Integrity of Inter-testicular Cell Junctions. J BiolChem 1997; 272: 6499–509.

(20) Johnston DS, Olivas E, DiCandeloro P, Wright W. Stage-Specific Changes in GDNF Expression by Rat Sertoli Cells: A Possible Regulator of the Replication and Differentiation of Stem Spermatogonia. Biol. Reprod 2011; 85: 763–9.

(21) Tapanainen JS, Aittomäki K, Min J, Vaskivuo T, Huhtaniemi I. Men homozygous for an inactivating mutation of the follicle-stimulating hormone (FSH) receptor gene present variable suppression of spermatogenesis and fertility. Nature Genetics 1997; 15,: 205–6.

(22) Naz RK, Rajesh PB. Role of tyrosine phosphorylation in sperm capacitation / acrosome reaction. Reprod Biol Endocrinol 2004; 2: doi: 10.1186/1477-7827-2-75 .

(23) Huo S, Du W, Shi P, Si Y, Zhao S. The role of spermatogenesis-associated protein 6 in testicular germ cell tumors. Int J clin Exp Pathol 2015; 8: 9119–25.

(24) Kamel AH, Foaud MA, Moussa HM. The adverse effects of bisphenol A on male albino rats.

The Journal of Basic and Applied Zoology 2018; 79: DOI 10.1186/s41936-018-0015-9.

(25) Reding SC, Stepnoski AL, Cloninger EW, Oatley JM. THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis. Reproduction 2010; 139: 893–903.

(26) Xuedong W, Kai L, Guangbo Z, Yuhua H, Jinxing L, Miao L, Lun Z, Caibin F, Jinxian P, Jianquan H, Hexing Y. B7-H3 promoted proliferation of mouse spermatogonial stem cells via the PI3K signaling pathway. Oncotarget 2018; 91542–52.

(27) Kathrein V, Andrej-Nikolai S. Human spermatogonial markers. Stem Cell Research 2017; 25: 300–9.

(28) Bokemeyer C, Kuezyk MA, DunnT, Serth J, Hartmann K, Jonasson J, Pietsch T, Jonas U, Schmoll H. Expression of stem-cell factor and its receptor c-kit protein in normal testicular tissue and malignant germ-cell tumors. J Cancer Res Clin Oncol1996; 122: 301–6.


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