CLINICOPATHOLOGICAL AND IMMUNOLOGICAL EFFECTS OF USING FORMALIZED KILLED VACCINE ALONE OR IN COMBINATION WITH PROPOLIS AGAINST Pasteurella multocida CHALLENGE IN RABBITS

Mohamed A. Hashem, Essam A. Mahmoud, Mohamed F.M. Farag

Abstract


Pasteurellosis is a common and troublesome issue of rabbits causing serious disorders. The immunization procedures are constantly the greatest preventive measures. In the present study, 40 New Zealand rabbits were used to investigate the protective efficacy of formalized Pasteurella multocida vaccine alone or in combination with propolis. The animals were divided into four equal groups (I-IV); negative control group, challenged non-vaccinated group, vaccinated challenged group and vaccinated propolis administered challenged group respectively. At the end of the 2nd and 6th weeks of the experiment, blood samples were collected from ear vein of rabbits for hematological, plasma, and serum examinations. The rabbits were then anaesthetized and sacrificed to collect tissue specimens from liver, kidneys, spleen and lungs for histopathological study. The results showed that using of propolis in combination with killed vaccine of Pasteurella multocida improved the immune response by increasing the leukocyte phagocytic activity against Pasteurella multocida (from 23.80% to 60.80%). Moreover, the clinicopathological findings including hemogram (RBCs count, Hb content, PCV, RBCs indices, platelets, total and differential leukocytes count), and hepato-renal function tests (ALT, AST, ALP, bilirubin, urea and creatinine), as well as, histopathological findings were better in infected rabbits treated with propolis- killed vaccine than using killed vaccine alone.

Key words: propolis; pasteurellosis; phagocytosis; nitric oxide; P. formalized killed vaccine


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(1) Zimmerman T., Deeb B., and DiGiacomo R. Polypeptides associated with Pasteurella multocida infection in rabbits. Am J of Vet Res 1992; 53: 1108–12.

(2) Gaertner D. Comparison of penicillin and gentamycin for treatment of pasteurellosis in rabbits. Lab Anim Sci 1991; 41: 78-80.

(3) Okerman L and Spanoghe L. Protective effects of inactivated pasteurella vaccines in specific pathogen free rabbits. Comparative Immunology, Microbiology and Infectious Diseases 1981; 4: 223–8.

(4) Okerman L and Spanoghe L. Immunity induced in mice by Past. multocida strain isolated from rabbits. Zentrablatt fur Veterinarmedizin 1980; 37 B (9/10): 759–63.

(5) Percy D, Prescott J and Bhasin J. Inactivated bacterial antigen were complemented with adjuvants in order to increase the immunogenicity of the vaccines. J Complementary Med 1985; 46: 227–9.

(6) Baljer G, Charcherr S and Mayr A. Efficacy and harmlessness of inactivated Past. multocida vaccines on subcutaneous, oral and intranasal application in mice. Zentralatt fur veterinarmedizine 1982; 27: 275–83.

(7) Shawky T. The immuno-potentiating Impact of zinc and vit.E in buffalo calves. Master thesis, Cairo University, Giza, Egypt 1996.

(8) Amal F. Pharmacological studies on Nigella sativa L on chickens genotoxic changes during LPS induced endotoxemia. Phytomedicine 1997; 20: 632–9.

(9) Abd El-Fattah A, Mohamed E, Mohamed E and Ramadan G. Effect of thymus extract on immunologic reactivity of chicken vaccinated with infectious bursal disease virus. Journal of Veterinary Medical Science 1999; 61:811–7.

(10) Boutabet K, Kebsa W, Alyane M and Lahouel M. Polyphenolic fraction of Algerian propolis protects rat kidney against acute oxidative stress induced by doxorubicin. Indian J. of Nephrology 2011; 21(2):101–6.

(11) Fischer G, Cleff M, Dummer L, Paulino N, Paulino A, Vilela C, Campos F, Storch T, Vargas D, Huber S and Vidor T. Adjuvant effect of green propolis on humoral immune response of bovines immunized with bovine herpesvirus type 5. Veterinary Immunology and Immunopathology 2007; 116: 79–84.

(12) El-Akabawy L, Zayan K, Tanatawy A and Omer R. Anticoccidial efficacy of propolis and toltrazuril against Eimeria stiedai in New Zealand white rabbits .Zag.Vet.J. 2004; 32:129–45.

(13) Cruickshank R, Duguid J, Marmion B and Swain R. Medical Microbiology, vol. 2, 12th edition. Churchill Livingstone, Edinburgh, UK 1975; 96–150.

(14) Osama A. Evaluation of the immune response against Past. multocida vaccines. Master thesis (Microbiology), Faculty of Vet. Med., Zagazig University 1997.

(15) Nassar S, Mohamed A, Soufy H, Nasr S, and Mahran K. Immunostimulant Effect of Egyptian Propolis in Rabbits. The Scientific World Journal 2012; 1–9.

(16) Coles E. Veterinary Clinical Pathology.4th edition. W.B. Saunders Company. Philadelphia 1986.

(17) Reitman S and Frankel S. Colorimetric method for determination of serum transaminases activities. Am J Clin Path 1957; 28: 56-63.

(18) Tietz N. Fundamentals of Clinical Pathology. W.B Saunders Company. Philadelphia 1976; 602–9.

(19) Fuehr J. Bilirubin determination of the serum according to the method of L. Jendrassik, R.A. Cleghorn and P. Grof. Med Monatsschr 1964; 18:183–4.

(20) Henry T. Clinical Chemistry .Principles and Techniques, 2nd edition. Harper and Row Publishers. New York 1974.

(21) Fawcett J and Scott J. A rapid and precise method for the determination of urea. Journal of Clinical Pathology 1960; 13:156.

(22) Montgomery H and Dymock J. The determination of nitrite in water. Analyst 1961; 86: 414–6.

(23) Waterstrat P, Ainsworth A and Capley G. Use of a discontinuous Percoll gradient technique for the separation of channel catfish peripheral blood leukocytes. J Fish Dis 1988; 11: 289–94.

(24) Ourth D and Chung K. Purification of antimicrobial factor from granules of channel catfish peripheral blood leucocytes. Biochemical and Biophysical Research Communications 2004; 313: 28–36.

(25) Ainsworth A and Chen D. Differences in the phagocytosis of four bacteria by channel catfish neutrophils. Dev. Comp. Immunol. 1990; 14: 201-9.

(26) Bancroft J, Stevens A and Turner D. Theory and Practice of Histological Technique, 4th edition. Churchill, Livingstone, New York, London, Sanfrancisco, Tokyo 1996.

(27) SPSS (2001): SPSS/PC + (2001), for the PC /XT. SPSS INC

(28) Pari L and Gnanasoundari M. Influence of naringenin on oxytetracycline mediated oxidative damage in rat liver. Basic and Clinical Pharmacology and Toxicology 2006; 98: 456–61.

(29) Orsatti C, Missima F, Pagliarone A and Sforcin J. Th1/Th2 cytokines’ expression and production by propolistreated mice. Journal of Ethnopharmacology 2010; 129: 314–8.

(30) Wang D, Hu Y, Sun J, Kong X, Zhang B, and Liu J. Comparative study on adjuvanticity of compound Chinese herbal medicinal ingredients. Vaccine 2005; 23: 3704–8.

(31) Manche H and Toll H. Pulmonary cavitation and massive hemorrhage caused by Pasteurella multocida. The new England Journal of Medicine 1964; 271:491–4.

(32) Jaglic Z, Jeklova E, Christensen H, Leva L, Register K, Kummer V, Kucerova Z, Faldyna M, Maskova J and Nedbalcova K. Host response in rabbits to infection with Pasteurella multocida serogroup F strains originating from fowl cholera. Can J Vet Res 2011; 75:200-8.

(33) Chung E, Abdullah F, Marza A, Saleh W, Ibrahim H, Abba Y, Zamri-Saad M, Haron A, Saharee A, Lila M and Norsidin M. Clinico-pathology and hematobiochemistry responses in buffaloes infected with Pasteurella multocida type B:2 immunogen outer membrane protein. Microbial Pathogenesis 2017; 11.

(34) Kleinrok Z, Borzecki Z, Scheller S and Matuga W. Biological properties and clinical application of propolis. X. Preliminary pharmacological evaluation of ethanol extract of propolis (EEP). Arzneimittel-Forschung 1987; 28 (2): 291–2.

(35) Jasprica I, Mornar A and Debeljak Z. In vivo study of propolis supplementation effects on antioxidative status and red blood cells. J Ethnopharmacology 2007; 110 (3): 548–54.

(36) Nassar S, Mohamed A, Soufy H and Nasr S. Protective Effect of Egyptian Propolis against Rabbit Pasteurellosis. BioMed. Research International 2013; 163724: 1–9.

(37) Youdim K, Shukitt-Hale B, MacKinnon S, Kalt W and Joseph J. Polyphenolics enhance red blood cell resistance to oxidative stress: in vitro and in vivo. Biochimica et Biophysica Acta 2000; 1523: 117–22.

(38) Fiorani M, Accorsi A and Cantoni O. Human red blood cells as a natural flavonoid reservoir. Free Radical Research 2003; 37: 1311–38.

(39) Kumazawa S, Hamasaka T and Nakayama T. Antioxidant activity of propolis of various geographic origins. Food Chemistry 2004; 84: 329–39.

(40) Russo A, Cardile V, Sanchez F, Troncoso N, Vanella A and Garabino J. Chilean propolis: antioxidant activity and antiproliferative action in human tumor cell lines. Life Sciences 2004; 76: 545–58.

(41) Galati G and O’Brien P. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radical Biology and Medicine 2004; 37: 287–303.

(42) Orsolić N and Basic I. Antitumor, hematostimulative and radioprotective action of water-soluble derivative of propolis (WSDP). Biomed. Pharmacother. 2005; 59: 561–70.

(43) Wu Q, Yub L, Qiu J, Shen B, DiWangb, Soromou L and Fenga H. Linalool attenuates lung inflammation induced by Pasteurella multocida via activating Nrf-2 signaling pathway International. Immunopharmacology 2014; 5:1567–9.

(44) Galdiero M, Palomba E, De L, Vitiello M and Pagnini P. Effects of the major Pasteurella multocida porin on bovine neutrophils. Am J Vet Res 1998; 59:1270–4.

(45) Cho S, Park J, Kim J and Yoon Y. Studies on the development of combined vaccine for control of snuffles (Past. multocida, Bordetella bronchiseptica infections). Research Report of Rural Development Administration Veterinary 1989; 31: 29–37.

(46) Borkowska O, Kedrak A and Truszczynki M. Pasteurella multocida serum level in rabbits vaccinated against pasteruellosis. Bulletin of the Veterinary Institute in Pulawy 1996; 40: 97–104.

(47) Borkowska O, Kedrak A and Truszczynki M. Application of the ELISA for determination of anti Pasteurella multocida IgG in sera of rabbit vaccinated against pasteurellosis under field condition. Bulletin of the Veterinary Institute in Pulawy 1997; (41): 17–24.

(48) Dimov V, Ivanovska N, Bankova V, Popov S, Manonlova N and Nikolov N. Immuno-modulatory action of propolis Influence on anti-infections protection and macrophage function. Apidologie 1991; 22: 155–62.

(49) Moriyasu J, Arai S, Motoda R, and Kurimoto M. In vitro activation of mouse macrophage by propolis extract powder. Biotherapy 1994; 8: 364–8.

(50) Dimov V., Ivanovska N., Bankova V., Popov S., Manonlova N. and Nikolov N. Immuno-modulatory action of propolis. Influnce on anti-infections protection and macrophage function. Apidologie 1991; 22: 155–62.

(51) Orsolic N and Basic I. Immuno-modulation by water soluble derivatives of propolis: a factor of anti-tumour reactivity. Journal of Ethnopharmacology 2003; 84: 265–73.

(52) Bratter C, Tregel M, Liebenthal C and Volk H. Prophylactic effectiveness of propolis for immunostimulation: a clinical pilot study. Forsch Komplementarmed 1999; 6: 256–60.

(53) Possamai M, Honorio-França A, Reinaque A, França E and Souza Souto P: Brazilian Propolis. A Natural Product That Improved the Fungicidal Activity by Blood Phagocytes. BioMed. Research International 2013; 54: 1018–27.

(54) Jarvinen L, Hogenesch H, Suckow M and Bowersock T. Induction of protective immunity in rabbits by coadministration of inactivated Pasteurella multocida toxin and potassium thiocyanate extract. Infection and Immunity 1998; 66: 3788–95.

(55) Thurston J, Cheville N, Rimler R and Sacks J. Serum complement activity and serum enzymes in rats after a subcutaneous injection of toxin prepared from Pasteurella multocida type D. Vet Immunol Immunopathol 1989; 23 (3-4): 385–8.

(56) Morsy A, Abdalla A, Soltan Y, Sallam S, El-Azrak K, Louvandini H and Alencar S. Effect of Brazilian red propolis administration on hematological, biochemical variables and parasitic response of Santa Inês ewes during and after flushing period. Trop. Anim. Health Prod. 2013; 45:1609–18.

(57) Mathy N, Mathy J, Lee R, Walker J, Lofthouse S and Meeusen E. Pathological and immunological changes after challenge infection with pasteurella multocida in naïve and immunized calves. Veterinary immunology and immuno-pathology 2002; 85: 179–88.




DOI: http://dx.doi.org/10.26873/SVR-631-2018

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