FFECT OF SOME FEED-ADDITIVES ON THE GROWTH PERFORMANCE, PHYSIOLOGICAL RESPONSE AND HISTOPATHOLOGICAL CHANGES OF RABBITS SUBJECTED TO OCHRATOXIN-A FEED CONTAMINATION
This experiment aimed to evaluate the toxic influence of ochratoxin- A (OTA) feed contamination and the effectiveness of some feed-additives (Humic acids, Bio-Plus 2B, Bio-Mos) in detoxifying ochratoxicosis. Thirty five day old weaned Animal Production Research Institute (APRI) rabbits were selected and were allocated to four groups. Group 1 (control, OTA group): rabbits were fed basal diet (ochratoxin- A level: 19 μg /kg diet). Group 2: rabbits were fed a basal diet containing 0.2 % humic acid. Group 3: rabbits were fed a basal diet containing 0.4 mg/kg diet Bacillus subtilis and Bacillus licheniformis (Bio-Plus 2B). Group 4: rabbits were fed a basal diet containing 0.1% Manna oligosaccharides (Bio-Mos). The control group showed significantly reduced feed intake, daily weight gain and growth performance index. Furthermore, there was a significant increase in AST, ALT, malondialdehyde, creatinine, and urea. Moreover, Bio-Mos group revealed significantly increased serum Hemoglobin, PCV, RBCs, MCV and RBCs/ lymphocyte ratio, and significantly decreased serum SOD and CAT activities. Additionally, internal organs (liver, kidney and intestine) of groups received feed additives revealed less deteriorative change in histopathological investigations in comparison with the control group. In conclusion, supplementation of contaminated feed with ochratoxin-A by feed-additives improves feed intake and final body weight and reduces mortality rate of rabbits. In addition, it improves liver and kidney functions and reduces its pathological changes, and restores antioxidant to its normal level.
Key words: rabbits; ochratoxin-A; growth performance; anti-oxidant
Bennett JW, Klich M. (2003) Mycotoxins. Clin Microbiol Rev 2003; 16: 497â€“516.
Zia MA, Rahman KU, Sheikh MA, Khan IA. Chemically treated strain improvement of Aspergillus niger for enhanced production of glucose oxidase. Int J Agric Biol2010; 12: 964â€“6.
Stoev SD. Studies on carcinogenic and toxic effects of ochratoxin A in chicks. Toxins 2010; 2:649â€“64.
Paterson RR, Lima N. Toxicology of my-cotoxins. EXS 2010; 100: 31â€“63.
Greco MV, Pardo AG, Ludemann V, Mar-tino PE, Pose GN. Mycoflora and natural inci-dence of selected mycotoxins in rabbit and chinchilla feeds. Sci World J 2012; ID956056: 1â€“6.
Ramos-Cormenzana A, Juarez-Jimenez B, Garcia-Pareja MP. Antimicrobial activity of olive mill waste-waters (alpechin) and biotrans-formed olive oil mill wastewater. Int Biodeterior Biodegradation 1996; 38(3-4): 283â€“90.
Islam KMS, Schumacher A, Gropp JM. Humic acid substances in animal agriculture. Pak. J. Nutr.2005; 4: 126â€“34.
Rath NC, Huff WE, Huff GR. Effects of humic acid on broiler chickens. Poult Sci 2006; 85: 410â€“4.
Patrcia C, Anna L, Dionâ€Žz M. Prevention of ochratoxin A contamination of food and ochratoxin A detoxification by microorganisms â€“ A review. Czech J Food Sci 2010; 28: 465â€“74.
Thakaew R, Niamsup H. Inhibitory ac-tivity of Bacillus subtilis BCC 6327 metabolites against growth of aflatoxigenic fungi isolated from bird chili powder. Int J Biosci Biochem Bioinform 2013; 3: 27â€“32.
Foldes T, Banhegyi I, Herpai L, Varga L, Szigeti J. Isolation of Bacillus strains from the rhizosphere of cereals and in-vitro screening for antagonism against phytopathogenic, food-borne pathogenic and spoilage microorganisms. J Appl Microbiol 2000; 89: 840â€“6.
Faraj MK, Smith JE, Harran G. Aflatoxin biodegradation: effects of temperature and mi-crobes. Mycol Res 1993: 97:1388â€“92.
Sommartya T. Peanut Disease. Bangkok: Kasetsart University Publishing 1997.
Devegowda G, Raju MVLN, Swamy M. Mycotoxins: noval solution in their counterac-tion. Foodstuff 1998: 7:12â€“5.
DE Blas C, Mateos GG. Feed formula-tion. In the Nutrition of the Rabbit (eds de Blas C. and Wiseman J.), Editor CABI Publishing 1998; 241â€“53.
Raya AH, Abbas AM, Hussein MAA. Comparative studies on the efficiency of some plant protein sources to replace soybean meal in rations for broiler chicks performance and eco-nomic efficiency. J. Agric. Sci., Mansoura Univ.1991; 16: 2514â€“27.
North MO. Commercial Chicken. Pro-duction Annual. 2nd Edition, Production 6th Ed. Interstate Printers and Publishers. INC., USA 1981.
Jain NC. Essentials of Veterinary Hema-tology, Fifth ed. Lea and Fibiger, Philadelphia, USA 1993.
Beuge JA, Aust SD. 1978. Microsomal lipid peroxidation. In: In: Colowick, S.P., Kaplan, N.O. (Eds.), Methodes in Enymology 52. Academic Press, New York 1978;302â€“10.
Nishikimi M, Roa NA, Yogi K. The oc-currence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 1972; 46:849â€“54.
Abdelhady D, El-Abasy M, Abou-Asa S, Elbialy Z, Shukry M, Hussein A, Saleh A, El-Magd M. The ameliorative effect of Aspergillus awamori on aflatoxin B1-induced hepatic dam-age in rabbits, World Mycotoxin Journal 2017; 10(4): 363â€“73.
Bancroft JD, Gamble M. Theory and practice of histological techniques, 5th ed. Churchill Livingstone, Edinburgh, UK 2007; 800.
Duncan DB. Multiple range and multiple F-Test, Biometrics 1955; 11: 1â€“42.
Konrad I, RÃ¶schenthaler R. Inhibition of phenylalanine tRNA synthetase from B. subtilis by ochratoxin A. FEBS LeU.1977; 83: 341â€“7.
Mourao JL, Pinheiro V, Alves A, Guedes CM, Pinto L, Saavedra MJ, Spring P, Kocher A. Effect of mannan oligosaccharides on the per-formance, intestinal morphology and cecal fer-mentation of fattening rabbits. Anim. Feed Sci. Technol. 2006;126:107â€“20.
Kumar M, Dwivedi P, Sharma AK, Tel-ang AG, Patil RD, Singh ND, Sanga M. Ochra-toxin-A and citrinin induced biochemical changes in New Zealand White rabbits. Indian J. Vet. Pathol. 2007; 31: 135â€“9.
Kumar A, Jindal N, Shukla CL, Pal Y, Ledoux DR, Rottinghaus GE. Effect of ochra-toxin A on Escherichia coli - challenged broiler chicks. Avian Dis 2003; 47: 415â€“24.
Agawane, S.B., Lonkar, P.S. Effect of probiotic containing Saccharomyces on experi-mental ochratoxicosis in broilers: Hematobio-chemical studies. J. Vet. Sci. 2004; 5: 359â€“67.
Thacker HL, Carlton WW. Ochratoxin-A mycotoxicosis in the guinea pigs. Fd. Contaqm. Toxicol.1977; 15: 563â€“74.
Pfohl-Leszkowicz1 A, Manderville RA. Ochratoxin A: An overview on toxicity and carcinogenicity in animals and humans. Mol. Nutr. Food Res.2007; 51: 61â€“99.
Aziz NH, El-Aziz AMA, Omran RMA. Effects of T-2 mycotoxin on histopathological changes in rabbits. Biomed. Lett. 1995; 51: 271â€“81.
Shalini M. Studies on ochratoxin con-tamination and ochratoxicosis in rabbits. Ph.D. Thesis, Bhavnagar University, Bhavnagar, In-dia, 1996.
Zofair SM, Shalini M, Verma RJ. Ochra-toxin induced hemolysis in rabbits. Indian J Exp Biol 1996; 34: 592â€“3.
Raina JS, Roy KS, Balwant S. Biochemi-cal and Histochemical studies in experimental mycotoxicosis in chicks. Indian J. Anim. Sci. 1991; 61: 1276-â€“81.
Mir MS, Dwivedi P, Charan K. Ochra-toxin â€“ A induced acute toxicity in rabbits. In-dian J. Vet. Pathol. 1999; 23: 8â€“13.
Mir MS, Dwivedi P. Clinical studies on experimental sub acute ochratoxicosis in rab-bits. Indian J. Vet. Pathol. 2000; 24: 99â€“101.
Anitha N. Pathology of ochratoxicosis and its amelioration with herbal oil and toxin binder in broiler chicken. M.V.Sc. thesis. Univ. KVAFSU,Bangalore, 2007.
AmÃ©zqueta S, GonzÃ¡lez-PeÃ±as E, Muril-lo-
Arbizu M, LÃ³pez de Cerain A. Ochratoxin A decontamination A review. Food Control 2009; 20:326â€“33.
Bayman P, Baker JL. Ochratoxins: A global perspective. Mycopathologia 2006; 162(3): 215â€“23.
Manoj K, Prabhaker D, Anil KS, Nittin DS, Rajendra DP. Ochratoxin A and citrinin nephrotoxicity in New Zealand White rabbits: an ultrastructural assessment. Mycopathologia 2007; 163: 21â€“30.