OCCURRENCE AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM BETA-LACTAMASE PRODUCING Enterobacteriaceae IN MILK AND SOME DAIRY PRODUCTS

Walaa Gaffer, Mayada Gwida, Randa Abo Samra, Maha Al- Ashmawy

Abstract


In the present study a total of one hundred samples were randomly purchased from different supermarkets in Damietta city, Egypt, including 25 samples each of raw milk, ice cream, Kareish and Domiati cheese. The collected samples were screened initially for cefotaxime-resistant bacteria using selective enrichment. The suspected colonies were further characterized by analysis of resistance genes in ESBL-producing isolates. A total of 59 ESBLs producers colonies (24 from raw milk, 15 from kareish cheese, 13 from Domiati cheese and 7 from ice cream) were recovered and identified as Escherichia coli (n = 29), Klebsiella pneumonia (n = 8), Klebsiella oxytoca (n = 5), Enterobacter aerogenes (n =8), Citrobacter diversus  (n = 8) and Serratia liquefaciens (n = 1). Resistance to two or more antibiotics was observed among the recovered isolates. E. coli isolates showed high resistance pattern against cefaclor (100%), cefoxitin (100%), cefalaxim (96.5%), ceftazidime (93%), pencillin (93%) and 79% for Pipercillin/Tazobactam. All the recovered Klebseilla spp. isolates were resistant to Cefepime and Pipercillin/Tazobactam (84.6% each) and exhibited 100% resistant to other antimicrobials agents. Meanwhile, all the recovered isolates were sensitive to imipenem and meropenem. Gene encoding blaCTX-M1 was mostly predominant among screened genes, being present in 13 (48%) dairy samples for blaTEM and blaSHV, they were detected in 12 (44%) and 4 (14.8%) isolates, respectively. In conclusion, there is clear evidence of circulation of antibiotic-resistant food borne ESBL producing Enterobacteriaceae in the examined dairy samples. The concern about increasing the risk of dissemination of such multi-drug resistant pathogens rises with a potential asymptomatic colonization and complication of systemic infection in human subjects. There is also a possible interface for the exchange of resistance genes within and across species and with commensal bacteria of the human and animals.

Key words: dairy products; ESBL producing Enterobacteriaceae; Egypt; public health


Full Text:

PDF

References


Odenthal S , Akineden Ö & Usleber E. Extended-spectrum β-lactamase producing En-terobacteriaceae in bulk tank milk from German dairy farms. International journal of food mi-crobiology 2016; 238: 72–8.‏

Horigan V , Kosmider R D , Horton R A , Randall L & Simons R. R. L. . An assessment of evidence data gaps in the investigation of possi-ble transmission routes of extended spectrum β-lactamase producing Escherichia coli from live-stock to humans in the UK. Preventive veteri-nary medicine 2016; 124: 1–8.‏

Braun SD, Ahmed MFE, El-Adawy H, Hotzel H, Engelmann I, Weiß D, Monecke S &Ehricht R . Surveillance of Extended-Spectrum Beta-Lactamase-Producing Escherich-ia coli in Dairy Cattle Farms in the Nile Delta, Egypt. Front. Microbiol. 7:1020. doi: 10.3389/ fmicb. 2016; 01020.

OIE .Terrestrial Animal Health Code. Risk Analysis for Antimicrobial Resistance Arising from the Use of Antimicrobials in Ani-mals. Version7 2014.

Hammad A M, Ishida Y & Shimamoto T. Prevalence and molecular characterization of ampicillin-resistant Enterobacteriaceae isolated from traditional Egyptian Domiati cheese. Jour-nal of food protection 2009; 72 (3): 624–30.‏

Seiffert S N , Hilty M , Perreten V & Endimiani A. Extended-spectrum cephalospor-in-resistant Gram-negative organisms in live-stock: an emerging problem for human health? Drug Resistance Updates 2013; 16 (1-2): 22–45.‏

Dahmen S, Métayer V, Gay E, Madec J Y & Haenni M. Characterization of extended-spectrum beta-lactamase (ESBL)-carrying plas-mids and clones of Enterobacteriaceae causing cattle mastitis in France. Veterinary microbiolo-gy 2013; 162 (2-4): 793–99.‏

Zeinhom M M & Abdel-Latef G. K. Pub-lic health risk of some milk borne pathogens. Beni-Suef University Journal of Basic and Ap-plied Sciences 2014; 3(3): 209–15.‏

Roberts D& Green wood M. Practical Food Microbiology, 3rd. Section 10: confirmato-ry biochemical testes. Blackwell 2003.

Kok T ,Worswich D & Gowans E . Some serological techniques for microbial and viral infections. In Practical Medical Microbiol-ogy (Collee J , Fraser A , Marmion B and Sim-mons A , eds.) 14th ed., Edinburgh, Churchill Livingstone, UK 1996.

CLSI. Performance Standards for Anti-microbial Susceptibility Testing. 27th ed. CLSI supplements M100. Wayne, PA: Clinical and Laborat ory Standards Institute 2017.

Ogutu J, Zhang Q, Huang Y, Yan H, Su L, Gao B, Zhang W, Zhao J, Cai W, Li W, Hong Zhao H, Chen Y, Song W, Chen X, Fu Y & Zhang F. Development of a multiplex PCR sys-tem and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaO-XA-1 group genes in clinical Klebsiella pneu-moniae and E. coli strains. The J. of Antibiotics 2015 ; 68: 725–33.

Perez F, Jones H, Hanson N & Geyer C. Global challenge of multidrug-resistant Aci-netobacter baumannii. Antimicrob. Agents Chemother. 2007; 51: 3471–84.

Skočková A , Bogdanovičoá K , Koláčková I & Karpíšková R . Antimicrobial-Resistant and Extended-Spectrum β-Lactamase–Producing Escherichia coli in Raw Cow's Milk. Journal of food protection 2015; 78(1): 72–7.‏

Geser N , Stephan R & Hächler H. Oc-currence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enter-obacteriaceae in food producing animals, minced meat and raw milk.

BMC veterinary research 2012; 8(1): 21.‏

Tekiner İ H & Özpınar H. Occurrence and characteristics of extended spectrum beta-lactamases-producing Enterobacteriaceae from foods of animal origin. brazilian journal of mi-crobiology 2016; 47(2): 444–51.‏

Badri A M , Ibrahim I T , Mohamed S G , Garbi M I , Kabbashi, A S & Arbab M. H. Prev-alence of extended spectrum beta lactamase (ESBL) producing Escherichia coli, and Klebsiella pneumoniae isolated from raw milk samples in Al Jazirah state, Sudan. Mol. Biol, 2017: 7(1000201), 10-4172.‏

Vrabec M , Lovayová V , Dudriková K , Gallo J & Dudriková, E. Antibiotic resistance and prevalence of Enterococcus spp. and Esch-erichia coli isolated from bryndza cheese. Ital-ian Journal of Animal Science 2015; 14(4): 3968.‏

Koovapra S , Bandyopadhyay S , Das G , Bhattacharyya D , Banerjee J , Mahanti A & Dimri, U. Molecular signature of extended spec-trum β-lactamase producing Klebsiella pneu-moniae isolated from bovine milk in eastern and north-eastern India. Infection, Genetics and Evolution 2016; 44: 395–402.‏

Talaat M , El-Shokry M , El-Kholy J , Ismail G , Kotb S , Hafez S & Lessa F. C. Na-tional surveillance of health care–associated infections in Egypt: developing a sustainable program in a resource-limited country. Ameri-can journal of infection control 2016 ; 44(11): 1296–301.‏

Pitout J D& Laupland KB . Extended-spectrum β-lactamase-producing Enterobacteri-aceae: an emerging public-health concern. The Lancet infectious diseases 2008; 8(3): 159–66.

Amador P , Fernandes R , Prudêncio C & Brito L. Resistance to β-lactams in bacteria iso-lated from different types of Portuguese cheese. International Journal of Molecular Sciences 2009; 10 (4): 1538–155.

Warjri I , Dutta T K , Lalzampuia H & Chandra, R. Detection and characterization of extended-spectrum β-lactamases (blaCTX-M-1 and blaSHV) producing Escherichia coli, Sal-monella spp. and Klebsiella pneumoniae isolat-ed from humans in Mizoram. Veterinary world 2015; 8(5): 599.

El-Domany R, Emara M, El-Magd M, Moustafa W, Abdeltwab N. Emergence of imipenem-resistant Pseudomonas aeruginosa clinical isolates from Egypt coharboring vim and imp carbapenemases. Microbial Drug Re-sistance 2017; 23:682–6.




DOI: http://dx.doi.org/10.26873/SVR-785-2019

Refbacks

  • There are currently no refbacks.


SLOVENIAN VETERINARY RESEARCH, Veterinary Faculty
Gerbičeva 60, SI-1000 Ljubljana, Slovenia, T: +386 (0)1 47 79 100, F: +386 (0)1 28 32 243, E: slovetres@vf.uni-lj.si
Published by computing.si