EFFECTS OF DIET’S ACIDIFYING ADDITIVES ON THE INTESTINAL HISTOMORPHOLOGY IN DUCKS
This study aimed at evaluating the effect of acidifying additives as formic and propionic acids on weight gain and on small intestinal macroscopic, microscopic and SEM morphology in ducks. A total of 30 Mulard ducklings were used in this study and they were randomly assigned to control (given standard commercial duck diets with no acidifiers added), 1% acidifiers added to the standard diet or 2% acidifiers added to the standard diet. After rearing the ducks for 52 days, their weight was recorded and then the length of the different parts of the small intestine was measured. Then, samples were taken for histological and scanning electron microscopical (SEM) examination of the small intestine. The present results found that addition of acidifying mixture to the diet significantly improved duck’s weight (P<0.05), also there were significant changes in length, histology and SEM characteristics of the small intestine between different duck groups. The observed effects were dose dependent, i.e. with more organic acids in the diet, the more the changes were noticeable. Our results indicate that, the addition of organic acids to duck’s diet increased small intestine’s length and increased the length of intestinal villi, possibly resulting in more surface area for digestion and absorption of the diet, and, therefore, greatly improved the final body weight of the study ducks.
Key words: acidified diets; organic acids; histology; intestinal villi; SEM
Byrd JA, Hargis BM, Caldwell DJ, Bailey RH, Herron KL, McReynolds JL, Brewer RL, Anderson RC, Bischoff KM, Callaway TR, Kubena LF. Effect of lactic acid administration in the drinking water during preslaughter feed withdrawal on Salmonella and Campylobacter contamination of broilers. Poultry Science. 2001; 80:278â€“83.
Chaveerach P, Keuzenkamp DA, Urlings HA, Lipman LJ, van Knapen F. In vitro study on the effect of organic acids on Campylobacter jejuni/coli populations in mixtures of water and feed. Poultry Science. 2002; 81:621â€“28.
Dibner JJ, Buttin P. Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism. Journal of Applied Poultry Research. 2002; 11:453â€“63.
Samanta S, Haldar S, Ghosh TK. Comparative efficacy of an organic acid blend and bacitracin methylene disalicylate as growth promoters in broiler chickens: Effects on performance, gut histology, and small intestinal milieu. Veterinary Medicine International. 2010:1â€“8.
Samli HE, Sirci G, Lorenzo Bermejo J, Koc F, Agma Okur A, Senkoylu N. Effect of addition of a commercial organic acid mixture on feed microbiology according to duration and conditions of storage. Acta Agriculturae Scandinavica A: Animal Sciences. 2008; 58:186â€“90.
Chaveerach P, Keuzenkamp DA, Lipman LJA, Van Knapen F. Effect of Organic Acids in Drinking Water for Young Broilers on Campylobacter Infection, Volatile Fatty Acid Production, Gut Microflora and Histological Cell Changes. Poultry Science. 2004; 83:330â€“34.
HernÃ¡ndez F, GarcÃa V, Madrid J, Orengo J, CatalÃ¡ P, MegÃas MD. Effect of formic acid on performance, digestibility, intestinal histomorphology and plasma metabolite levels of broiler chickens. British Poultry Science. 2006; 47:50â€“56.
Yamauchi K. Review on Chicken Intestinal Villus Histological Alterations Related with Intestinal Function. The Journal of Poultry Science. 2002; 39:229â€“42.
Jansen W, Reich F, Klein G. Large-scale feasibility of organic acids as a permanent preharvest intervention in drinking water of broilers and their effect on foodborne Campylobacter spp. before processing. Journal of Applied Microbiology. 2014; 116:1676â€“87.
Choi PM, Sun RC, Guo J, Erwin CR, Warner BW. High-Fat Diet Enhances Villus Growth During the Adaptation Response to Massive Proximal Small Bowel Resection. Journal of Gastrointestinal Surgery. 2014; 18:286â€“94.
Park JH, Grandjean CJ, Hart MH, Baylor JM, Vanderhoof JA. Effects of Dietary Linoleic Acid on Mucosal Adaptation after Small Bowel Resection. Digestion. 1989; 44:57â€“65.
Sukhotnik I, Lerner A, Sabo E, Krausz MM, Siplovich L, Coran AG, Mogilner J, Shiloni E. Effects of Enteral Arginine Supplementation on the Structural Intestinal Adaptation in a Rat Model of Short Bowel Syndrome. Digestive Diseases and Sciences. 2003; 48:1346â€“51.
Tappenden KA. Mechanisms of Enteral Nutrient-Enhanced Intestinal Adaptation. Gastroenterology. 2006; 130:S93â€“99.
Bohorquez D V., Bohorquez NE, Ferket PR. Ultrastructural development of the small intestinal mucosa in the embryo and turkey poult: A light and electron microscopy study. Poultry Science. 2011; 90:842â€“55.
Fischer ER, Hansen BT, Nair V, Hoyt FH, Dorward DW. Scanning Electron Microscopy. Curr. Protoc. Microbiol., vol. 25, Hoboken, NJ, USA, John Wiley & Sons, Inc. 2012; 25vol., p. 2B.2.1-2B.2.47.
Ding BA, Pirone A, Lenzi C, Xiaoming N, Baglini A, Romboli I. Histochemical features of the Muscovy duck small intestine during development. Tissue and Cell. 2011; 43:190â€“95.
Ruttanavut J, Yamauchi K, Goto H, Erikawa T. Effects of Dietary Bamboo Charcoal Powder Including Vinegar Liquid on Growth Performance and Histological Intestinal Change in Aigamo Ducks. International Journal of Poultry Science. 2009; 8:229â€“36.
Khambualai O, Ruttanavut J, Kitabatake M, Goto H, Erikawa T, Yamauchi K. Effects of dietary natural zeolite including plant extract on growth performance and intestinal histology in Aigamo ducks. British Poultry Science. 2009; 50:123â€“30.
Partanen KH, Mroz Z. Organic acids for performance enhancement in pig diets. Nutrition Research Reviews. 1999; 12:117â€“45.
Noy Y, Sklan D. Digestion and Absorption in the Young Chick. Poultry Science. 1995; 74:366â€“73.
Jamroz D, Wiliczkiewicz A, Orda J, Wertelecki T, Skorupinska J. Aspects of development of digestive activity of intestine in young chickens, ducks and geese. Journal of Animal Physiology and Animal Nutrition. 2002; 86:353â€“66.
Yamauchi KE, Isshiki Y. Scanning electron microscopic observations on the intestinal villi in growing white leghorn and broiler chickens from 1 to 30 days of age. British Poultry Science. 1991; 32:67â€“78.
Garcia V, Catala-Gregori P, Hernandez F, Megias MD, Madrid J. Effect of Formic Acid and Plant Extracts on Growth, Nutrient Digestibility, Intestine Mucosa Morphology, and Meat Yield of Broilers. The Journal of Applied Poultry Research. 2007; 16:555â€“62.
Paul SK, Halder G, Mondal MK, Samanta G. Effect of Organic Acid Salt on the Performance and Gut Health of Broiler Chicken. The Journal of Poultry Science. 2007; 44:389â€“95.
Witlock DR, Wyatt RD, Ruff MD. Morphological changes in the avian intestine induced by citrinin and lack of effect of aflatoxin and T-2 toxin as seen with scanning electron microscopy. Toxicon. 1977; 15:41â€“44.
Kadhim KK, Bakar MZA, Noordin MM, Babjee MA, Saad MZ. Light and scanning electron microscopy of the small intestine of young Malaysian village chicken and commercial broiler. Pertanika Journal of Tropical Agricultural Science. 2014; 37:51â€“64.