Abd Ellwahed El-Shawadfi, Fares El-Khyat, AbdEl-Galil El-Gohary


The aim of the present study was to identify virulent Newcastle disease viruses (NDVs) circulating in broilers in Kafrelsheikh Governorate during the year 2017. Recognition of the virulent NDV strains to identify their degree of identity with the currently used vaccines against Newcastle disease in order to predict degree of protective immunity that can be offered by traditionally vaccines used against these virulent NDVs. The obtained results revealed presence of 11 samples positive for NDV as detected by inoculation into chick embryo via allantoic cavity route followed by HA and HI test. Furthermore, by using specific primers for F region to detect genotype VII, only 4 out of the positive 11 samples belonged to class II genotype VII sub genotype d were detected.

Key words: Newcastle disease virus; genotype VII; broilers; Egypt

Full Text:



Doyle T M. A hither to unrecorded disease of fowls due to filter passing virus. J. Comp. Pathol 1927; 40:144–69.

Daubney R and Mansi W. The occurrence of Newcastle disease virus in Egypt. J. Comp. Pathol 1947; 58: 189-200.

Ballagi A, Wehmann E, Herczeg J, Belfik S, and Lomniczi B. Identification and grouping of Newcastle disease virus strains by restriction site analysis of a region from the F gene. Arch Virol 1996; 141:243 –61.

Radwan MM, Darwish SF, El-Sabagh IM, El-Sanousi AA, Shalaby MA. Isolation and mo-lecular characterization of Newcastle disease virus genotypes II and VIId in Egypt between 2011 and 2012. Virus Genes 2013; 47(2):311–6.

Beard CW and Easterday B C. The influ-ence of route of administration of Newcastle disease virus on host response. J Infect Dis 1967; 117:55–70.

Higgins D A. Nine disease outbreaks asso-ciated with myxoviruses among ducks in Hong Kong. Trop Anim Health Prod 1971; 3:232–40.

Westbury HA. Virulent Newcastle disease in Australia: molecular epidemiological analysis of viruses isolated prior to and during the out-breaks of 1998-2000. Virus Res. 2001; 77 (1), 51–60.

Young M, Alders R, Grimes S et al. Con-trolling Newcastle Disease in Village Chickens: A Laboratory Manual. ACIAR Monograph 2002; 87: 142–9.

Alexander DJ and Senne DA. Newcastle disease and other avian paramyxoviruses: A laboratory manual for the isolation, identifica-tion and characterization of avian pathogens, 5th edition, 2008; 135–41.

Miller PJ, Decanini EL and Afonso CL. Newcastle disease: Evolution of genotypes and the related diagnostic challenges. Infect. Genet. Evol. 2010; 10 (1), 26–35.

Pollard JW and Walker JM. Methods in molecular biology: Plant cell and tissue culture. Humana Press, Totowa 1997; 6: 129–39.

Wise MG, Suarez DL, Seal BS et al. De-velopment of a Real-Time Reverse-Transcription PCR for Detection of Newcastle Disease Virus RNA in Clinical Samples. Journal of Clinical Microbiology 2004; 42 (1): 329–38.

Mase M, Imai K, Sanada Y et al. Phylo-genetic analysis of Newcastle disease virus gen-otypes isolated in Japan. J. Clin. Microbiol. 2002; 40: 3826–30.

Orabi, A., Hussein, A., Saleh, A.A., El-Magd, M.A., Munir, M., 2017. Evolutionary insights into the fusion protein of Newcastle disease virus isolated from vaccinated chickens in 2016 in Egypt. Archives of Virology 162, 3069–79.

Aldous EW, Mynn JK, Banks J and Al-exander DJ. A molecular epidemiological study of avian paramyxovirus type 1 (Newcastle dis-ease virus) isolates by phylogenetic analysis of a partial nucleotide sequence of the fusion pro-tein gene. Avian Pathol. 2003; 32:239–57.

Hussein HA, Emara MM and Rohaim MA. Molecular Characterization of Newcastle Disease Virus Genotype VIID in Avian influen-za H5N1 Infected Broiler Flock in Egypt. Inter-national Journal of Virology 2014; 10 (1): 46–54.

Sultan S, Osman N, Ahmed A, Ibrahim R and Sabra M. Phylogenetic Characterization of Velogenic Newcastle Disease Viruses Isolated from Field Outbreaks among Vaccinated broiler chickens in Upper Egypt. Assiut Vet Med J 2015; 61: 126–35.



  • There are currently no refbacks.

Gerbičeva 60, SI-1000 Ljubljana, Slovenia, T: +386 (0)1 47 79 100, F: +386 (0)1 28 32 243, E:
Published by