THE INFLUENCE OF DIFFERENT MOLECULAR WEIGHT SEMINAL PLASMA PROTEIN CONTENT ON SOME FERTILITY PARAMETERS IN BOAR'S EJACULATES
DOI:
https://doi.org/10.26873/SVR-738-2019Abstract
The aim of this study was to investigate the effect of different percentage of seminal plasma proteins with different molecular weight on sperm motility and fertility parameters (farrowing rate (FR), number of live-born pigs (PBA) per litter and percentage of unsuccessful insemination). A total of 50 sperm-rich ejaculate fractions were collected (one per boar) using the gloved hand method. The quality parameters of the semen samples were first evaluated at the farm. Further assessment of sperm quality was performed on a CASA - computer assisted semen analysis by two competent operators. Seminal plasma protein fractions were obtained by AOAC - Association of Official Analytical Chemists as a chemical method. The assessment of reproductive performance was carried out based on collected data of three parameters in selected 9696 sows: FR, PBA per litter and percentage of unsuccessful insemination. Protein fractions were divided in to three groups (10 – 20kDa, 21 – 30kDa and 31 – 40kDa) Proteins with 10 – 20kDa did not have significant effect and correlation with analyzed parameters. Significant differences were recorded in farrowing rate between samples with up to 80 % compared to samples with 10% of proteins with 21 – 30kDa. Significant differences were recorded in unsuccessful insemination between samples with different percentage of proteins with 31 – 40kDa. Results of this study have shown the effect of different percentage of certain fraction of seminal plasma proteins on boar ejaculates fertility potential.
Key words: boars; semen quality; seminal plasma proteins; reproductive results
VPLIV VSEBNOSTI BELJAKOVIN SEMENSKE PLAZME RAZLIČNIH MOLEKULARNIH TEŽ NA NEKATERE PARAMETRE PLODNOSTI V EJAKULATIH MERJASCEV
Povzetek: Cilj opisane raziskave je bil proučiti vpliv prisotnosti beljakovin semenske plazme z različno molekulsko maso na gibljivost semenčic in parametre plodnosti kot so: stopnja prasitev, število živorojenih prašičev na leglo in odstotek neuspešnih osemenitev. Po metodi z orokavičeno roko je bilo zbranih 50 frakcij ejakulata, bogatih s semenčicami (1 na merjasca). Na kmetiji smo najprej ocenili parametre kakovosti vzorcev semena. Nadaljnja ocena kakovosti semenčic je bila izvedena z računalniško podprto analizo semena (CASA), ki sta jo izvedla dva usposobljena operaterja. Semenske frakcije beljakovin v plazmi so bile pridobljene s pomočjo AOAC - metode. Ocena reproduktivne učinkovitosti je bila izvedena na podlagi zbranih podatkov treh parametrov pri izbranih 9696 svinjah. Proteinske frakcije so bile razdeljene v tri skupine (10 – 20 kDa, 21 – 30 kDa in 31 – 40 kDa). Beljakovine velikosti 10 - 20 kDa niso imele značilnega učinka in soodvisnosti z analiziranimi parametri. Ugotovili pa smo statistično značilne razlike v stopnji prasitve med vzorci z do 80 % beljakovin velikosti 21 - 30kDa v primerjavi z vzorci s samo 10 % beljakovin velikosti 21 - 30kDa. Statistično značilne razlike so bile ugotovljene tudi pri uspešnosti osemenitev med vzorci z različnim odstotkom beljakovin velikosti 31 - 40kDa. Rezultati te študije kažejo vpliv različnega odstotka določenega deleža beljakovin iz semenske plazme na potencial plodnosti merjascev.
Ključne besede: merjasci; kakovost semena; semenske beljakovine v plazmi; reprodukcijski rezultati
References
(1.) Koketsu, Y, Dial GD, King VL. Influence of various factors on farrowing rate on farms using early weaning. J Anim Sci 1997; 75(10): 2580–7.
(2.) Khalifa T, Rekkas C, Samartzi F, et al. Highlights on artificial insemination (AI) technology in the pigs. Maced Vet Rev 2014; 37(1): 5–34.
(3.) Apić J, StanÄić I, Vakanjac S, et al. Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days. Anim Reprod 2016; 13(1): 36–41.
(4.) Broekhuijse ML, Šoštarić E, Feitsma H, Gadella BM. The value of microscopic semen motility assessment at collection for a commercial artificial insemination center, a retrospective study on factors explaining variation in pig fertility. Theriogenology 2012; 77(7): 1466–79.
(5.) Khalifa TAA, Tsakmakidis IA. A preliminary study on preservability of porcinesemen in INRA-96 extender. In: Proceeding of the 17th International Congress on Biotechnology in Animal Reproduction (ICBAR). Leipzig : Anhalt University of Applied Sciences, Germany, 2012.
(6.) Perez-Patino C, Barranco I, Parrilla I, et al. Characterization of the porcine seminal plasma proteome comparing ejaculate portions. J Proteom 2016; 142: 15–23.
(7.) Karunakaran M, Ratnakaran U, Naik PK, Chakurkar EB. Electrophoretic profile of boar seminal proteins. J Appl Anim Res 2016; 44(1): 403–5.
(8.) Druart X, Rickard JP Mactier S, et al. Proteomic characterization and cross species comparison of mammalian seminal plasma. J Proteom 2013; 91: 13–22.
(9.) Kordan W, Lecewicz M Tobolski J. Effect of platelet-activating factor on motility. Pol J Vet Sci 2009; 12(2): 175–81.
(10.) Troedsson MHT, Desvousges A, Alghamdi AS, et al . Components in seminal plasma regulating sperm transport and elimination. Anim Reprod Sci 2005; 89(1/4): 171–86.
(11.) Daskalova D, Kukov A, Kirilova I, Ivanova-Kicheva M. Protein analysis of boar seminal plasma proteins with protective effect during low-temperature storage of spermatozoa. Biotechnol Biotechnol Equip 2014; 28(4): 716–20.
(12.) Shipley CF. Breeding soundness examination of the boar. J Swine Health Prod 1999; 7(3): 117–20.
(13.) Kommisrud E, Paulenz H, Sehested E, Grevle IS. Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for five days. Acta Vet Scand 2002; 43(1): 49–55.
(14.) Strzezek J, Wysocki P, Kordan W, et al . Proteomics of boar seminal plasma–current studies and possibility of their application in biotechnology of animal reproduction. Reprod Biol 2005; 5(3): 279–90.
(15.) Strzezek J, Saiz-Cidoncha F, Wysocki P, et al. Seminal plasma proteins as markers of biological value of boar semen. Anim Sci Pap Rep 2002; 20(4): 255–66.
(16.) Novak S, Ruizâ€Sánchez A, Dixon WT, et al. Seminal plasma proteins as potential markers of relative fertility in boars. J Androl 2010; 31(2): 188–200.
(17.) Caballero I, Vazquez JM, GarcÃa EM, et al. Major proteins of boar seminal plasma as a tool for biotechnological preservation of spermatozoa. Theriogenology 2008; 70(8): 1352–5.
(18.) Barranco I, Tvarijonaviciute A, Perez-Patiño C, et al. Glutathione peroxidase 5 is expressed by the entire pig male genital tract and once in the seminal plasma contributes to sperm survival and in vivo fertility. PLoS ONE 2016; 11(9): e0162958 (1–10). https://doi.org/10.1371/journal.pone.0162958
(19.) Karunakaran M, Ratnakaran U, Naik PK, Chakurkar EB. Electrophoretic profile of boar seminal proteins. J Appl Anim Res 2016; 44(1): 403–5.
(20.) Flowers WL. Relationship between seminal plasma proteins and boar fertility. In: Annual Swine Report. Raleigh, NC : College of Agriculture and Life Sciences, Department of Animal Science, 2001: 1–4.
(21.) Žura Žaja I, Samardžija M, Vince S, et al. Differences in seminal plasma and spermatozoa antioxidative systems and seminal plasma lipid and protein levels among boar breeds and hybrid genetic traits. Anim Reprod Sci 2016; 170: 75–2.
(22.) Žura Žaja I, Samardžija M, Vince S, et al. Influence of boar breeds or hybrid genetic composition on semen quality and seminal plasma biochemical variables. Anim Reprod Sci 2016; 164: 169–76.
(23.) RodrÃguezâ€MartÃnez H, Kvist U, Ernerudh J, Sanz L, Calvete JJ. Seminal plasma proteins: what role do they play? Am J Reprod Immunol 2011; 66 (Suppl. 1): 11–22.
(24.) Töpferâ€Petersen E, Romero A, Varela PF, et al. Spermadhesins: a new protein family. Facts, hypotheses and perspectives. Andrologia, 1998; 30: 217–24.
