OCCURRENCE AND TRANSPLACENTAL TRANSMISSION OF Anaplasma marginale IN DAIRY CATTLE

Mudasir Nazar, Murad Ali Khan, Assar Ali Shah, Sadeeq Ur Rahman, Ikramullah Khan, Ahsan Ullah, Irfan Ullah Khan, Muhammad Shuaib

Abstract


Bovine anaplasmosis, caused by Anaplasma marginale, is a non-contagious tick borne disease. The main objective of the current study was to investigate comparative frequency of anaplasmosis in three different cattle breeds (European breed/ Holstein Friesian and Jersey breed, indigenous breed and cross breed (1st and 2nd pedigree) and transplacental transmission using real time polymerase chain reaction for detection. Of the total 96 blood samples analyzed, our results indicated an overall incidence 45.83% (44/96) of A. marginale with highest incidence 62.5% (20/32) in European breed, followed by 42.4% (14/33) in cross breed and 35.4% (11/31) in indigenous breed. Most importantly, our results indicated that 13.7% (4/29) calves were found positive for the presence of A. marginale indicating transplacental transmission. Furthermore, indirect ELISA revealed an overall incidence rate of 34.3% (33/96) more likely indicating current or previous exposure. Finally, Giemsa staining determined that 15% (15/96) animals were found positive by examining red blood cells. Statistical analysis showed significantly higher (P<0.05) incidence of European breed as compared to crossbreed and indigenous breed of cattle, while non-significant (P>0.05) difference was found among the crossbred and indigenous breed of cattle. Moreover, non significant (P> 0.05) effect of age group was observed on the incidence of A. marginale. White blood cell count and mean corpuscular volume were significantly (P<0.05) higher in infected cattle, while, red blood cells, packed cell volume hemoglobin concentration, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration (MCHC) were significantly (P<0.05) higher in non-infected as compare to infected animals.

Key words: Anaplasma marginale; cattle breeds; enzyme linked immuno sorbent assay; real-time polymerase chain reaction; transplacental transmission

 

POJAVNOST IN PRENOS Anaplasma marginale PREKO POSTELJICE PRI MLEČNEM GOVEDU

Povzetek: Goveja anaplazmoza, ki jo povzroča Anaplasma marginale, je bolezen, ki ni kužna in jo prenašajo klopi. Glavni namen študije je bil raziskati in primerjati pogostost anaplazmoze pri različnih pasmah goveda in sicer dveh evropskih pasmah (holstein-frizijske pasma in pasma jersey) pri avtohtoni pasmi ter pri križancih med evropskimi pasmami in avtohtonimi pasmami (1. In 2. generacija križancev) ter prenos preko posteljice z uporabo verižne rekacije s polimerazo v realnem času (PCR). Od skupno 96 pregledanih vzorcev krvi so rezultati pokazali skupno incidenco 45,83 % (44/96) A. marginale z največjo incidenco 62,5 % (20/32) pri evropskih pasmah, nato 42,4 % (14/33) pri križanih živalih in 35,4 % (11/31) pri avtohtonih pasmah. Rezultati so tudi pokazali pozitivno rekacijo na prisotnost A. marginale pri 13,7 % (4/29) telet, kar kaže na prenos anaplazmoze preko posteljice. Metoda posrednega testa ELISA, ki pokaže na trenutno ali predhodno izpostavljenost, je pokazala 34,3-odstotno pojavnost pri vseh živalih skupaj (33/96 živali). Proučitev rdečih krvničk z barvanjem Giemsa je pokazalo 15 % (15/96) pozitivnih živali. Statistična analiza je pokazala statistično značilno razliko v pojavnosti anaplazmoze med evropskima pasmama v primerjavi s križanci in avtohtono pasmo (p < 0,05), razlika med križanci in avtohtono pasmo goveda pa ni bila statistično značilna. Poleg tega je bil pri pojavnosti A. marginale opazen neznaten (statistično neznačilen) učinek starosti proučevanih živali. Število belih krvnih celic in povprečni volumen telesne mase sta bila pri okuženih govedih statistično značilno povečana (p < 0,05), medtem ko so bile rdeče krvne celice, koncentracija hemoglobina v celicah in povprečna količina hemoglobina v posameznem eritrocitu (MCHC) v neokuženi skupini višja kot v skupini z okuženimi živalmi (p <0,05).

Ključne besede: Anaplasma marginale; pasme goveda; test ELISA; verižna reakcija s polimerazo v realnem času; prenos preko posteljice


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References


(1) Jaswal H, Bal MS, Singla LD, et al. Application of msp1β PCR and 16S rRNA semi nested PCR-RFLP for detection of persistent anaplasmosis in tick infested cattle. Int J Adv Res 2014; 2: 188–96.

(2) Sharma A, Singla LD, Tuli A, Kaur P, Bal MS. Detection and assessment of risk factors associated with natural concurrent infection of Trypanosoma evansi and Anaplasma marginale in dairy animals by duplex PCR in eastern Punjab. Trop Anim Health Prod 2015; 47: 251–7.

(3) Jaswal H, Bal MS, Singla LD, Gupta K, Brar APS. Pathological observation on clinical Anaplasma marginale infection in cattle. J Parasit Dis 2015; 39: 495–8.

(4) McCallon BR. Prevalence and economic aspects of anaplasmosis. In: Proceedings of the Sixth National Anaplasmosis Conference. Stillwater : Heritage Press, 1973: 1–3.

(5) Kilpatrick AM, Randolph SE. Drivers, dynamics, and control of emerging vector-borne zoonotic diseases. Lancet 2012; 380: 1946–55.

(6) Grau HEG, Cunha Filho NAd, Pappen FG, Farias NAdR. Transplacental transmission of Anaplasma marginale in beef cattle chronically infected in southern Brazil. Rev Bras Parasitol Vet 2013; 22: 189–93.

(7) Léger E, Vourc hG, Vial L, Chevillon C, McCoy KD. Changing distributions of ticks: causes and consequences. Exp Appl Acarol 2013; 59: 219–44.

(8) Aubry P, Geale D. A review of bovine anaplasmosis. Transbound Emerg Dis 2011; 8: 1–30.

(9) Costa SCL, de Magalhães VCS, de Oliveira UV, et al. Transplacental transmission of bovine tick-borne pathogens: frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil. Ticks Tick Born Dis 2016; 7(2): 270–5.

(10) Kocan KM, de la Fuente J, Blouin EF, Coetzee J F, Ewing S. The natural history of Anaplasma marginale. Vet Parasitol 2010; 167: 95–107.

(11) Eriks IS, Palmer GH, McGuire TC, Allred DR, Barbet AF. Detection and quantitation of Anaplasma marginale in carrier cattle by using a nucleic acid probe. J Clin Microbiol 1989; 27: 279–84.

(12) Kieser ST, Eriks IS, Palmer GH. Cyclic rickettsemia during persistent Anaplasma marginale infection of cattle. Infect Immun 1990; 58: 1117–9.

(13) Madruga CR, Marques APC, Leal CRB, Carvalho CM, Araújo FR, Kessler RH. Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale. Pesq Vet Bras 2000; 20: 109–12.

(14) Atif FA, Khan MS, Iqbal HJ, Arshad GM, Ashraf E, Ullah S. Prevalence of Anaplasma marginale, Babesia bigemina and Theileria annulata infections among cattle in Sargodha District, Pakistan. Afr J Agric Res 2012; 7(22): 3302–7.

(15) Sajid MS, Iqbal Z, Khan MN, Muhammad G, Needham G, Khan MK. Prevalence, associated determinants, and in vivo chemotherapeutic control of hard ticks (Acari: Ixodidae) infesting domestic goats (Capra hircus) of lower Punjab, Pakistan. Parasitol Res 2011; 108(3): 601–9.

(16) Bhutto B, Gadahi JA, Khuhro A, et al. A survey on haemo-protozoan parasites in buffaloes of Landhi dairy colony, Karachi-Pakistan. Int J Agro Vet Med Sci 2012; 6: 73–6.

(17) Benjamin MM. Outline of veterinary clinical pathology. 3rd ed. Ames : Iowa State University Press, 1978.

(18) Khan M, Zahoor A, Jahangir M, Mirza MA. Prevalence of blood parasites in cattle and buffaloes. Pak Vet J 2004; 24: 193–5.

(19) Johnston L, Trueman K, Leatch G, Wilson A. A comparison of direct fluorescent antibody and giemsa staining for the post-mortem diagnosis of anaplasmosis. Aust Vet J 1980; 56: 116–8.

(20) Atif FA, Khan MS, Iqbal HJ, Arshad GM, Ashraf E, Ullah S. Prevalence of Anaplasma marginale, Babesia bigemina and Theileria annulata infections among cattle in Sargodha District, Pakistan. Afr J Agr Res 2012b; 7: 3302-3307.

(21) Awad H, Antunes S, Galindo RC, et al. Prevalence and genetic diversity of Babesia and Anaplasma species in cattle in Sudan. Vet Parasitol 2011;181: 146–52.

(22) Shebish E, Vemulapall, R, Oseto C. Prevalence and molecular detection of Anaplasma marginale, Babesia bovis and Babesia bigemina in cattle from Puntarenas Province, Costa Rica. Vet Parasitol 2012; 188: 164–7.

(23) Sharma A, Singla LD, Kaur P, Bal MS, Batth BK, Juyal PD. Prevalence and haemato-biochemical profile of Anaplasma marginale infection in dairy animals of Punjab (India). Asian Pac J Trop Med 2013; 6(2): 139–44.

(24) Marufu MC, Chimonyo M, Mtshali M, Dzama KG. Molecular prevalence of Anaplasma marginale (Rickettsiales: Anaplasmataceae) in Nguni and local crossbred cattle under the low input production system in South Africa. In: Proceedings of the 10th Annual Congress of the Southern African Society for Veterinary Epidemiology and Preventive Medicine, At Farm Inn, South Africa, 2012: 57.

(25) Alim MA, Das S, Roy K, et al. Prevalence of hemoprotozoan diseases in cattle population of Chittagong division, Bangladesh. Pak Vet J 2012; 32: 221–4.

(26) Hamou SA, Rahali T, Sahibi H, et al. Molecular and serological prevalence of Anaplasma marginale in cattle of North Central Morocco. Res Vet Sci 2012; 93: 1318–23.

(27) Morris C. A review of genetic resistance to disease in Bos taurus cattle. Vet J 2007; 174(3): 481–91.

(28) Atif F, Khan M, Iqbal H, Roheen T. Prevalence of tick-borne diseases in Punjab (Pakistan) and hematological profile of Anaplasma marginale infection in indigenous and crossbred cattle. Pak J Sci 2012; 64(1): 11–5.

(29) Fowler D, Swift BL. Abortion in cows inoculated with Anaplasma marginale. Theriogenology 1975; 4(2/3): 59–67.

(30) Zaugg J. Bovine anaplasmosis: transplacental transmission as it relates to stage of gestation. Am J Vet Res 1985; 46: 570–2.

(31) Grau HEG, Cunha Filho NAd, Pappen FG,Farias NAdR.Transplacental transmission of Anaplasma marginale in beef cattle chronically infected in southern Brazil. Revis Bras Para Vet 2013; 22: 189-193.

(32) Pohl AE, Cabezas-Cruz A, Ribeiro MFB, et al. Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil. Rev Bras Parasitol Vet 2013; 22: 129–35.

(33) Salabarria F, Pino R.Vertical transmission of Anaplasma marginale in cows affected in late pregnancy. Rev Cub Cienc Vet 1988; 19(3): 179–82.

(34) da Silva JB, de Santana Castro GN, Fonseca AH. Longitudinal study of risk factors for anaplasmosis and transplacental transmission in herd cattle. Semin Ciênc Agrár 2014; 35(4 Supl): 2491-500.




DOI: http://dx.doi.org/10.26873/SVR-499-2018

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