Mudasir Nazar, Murad Ali Khan, Assar Ali Shah, Sadeeq Ur Rahman, Ikramullah Khan, Ahsan Ullah, Irfan Ullah Khan, Muhammad Shuaib


Bovine anaplasmosis, caused by Anaplasma marginale, is a non-contagious tick borne disease. The main objective of the current study was to investigate comparative frequency of anaplasmosis in three different cattle breeds (European breed/ Holstein Friesian and Jersey breed, indigenous breed and cross breed (1st and 2nd pedigree) and transplacental transmission using real time polymerase chain reaction for detection. Of the total 96 blood samples analyzed, our results indicated an overall incidence 45.83% (44/96) of A. marginale with highest incidence 62.5% (20/32) in European breed, followed by 42.4% (14/33) in cross breed and 35.4% (11/31) in indigenous breed. Most importantly, our results indicated that 13.7% (4/29) calves were found positive for the presence of A. marginale indicating transplacental transmission. Furthermore, indirect ELISA revealed an overall incidence rate of 34.3% (33/96) more likely indicating current or previous exposure. Finally, Giemsa staining determined that 15% (15/96) animals were found positive by examining red blood cells. Statistical analysis showed significantly higher (P<0.05) incidence of European breed as compared to crossbreed and indigenous breed of cattle, while non-significant (P>0.05) difference was found among the crossbred and indigenous breed of cattle. Moreover, non significant (P> 0.05) effect of age group was observed on the incidence of A. marginale. White blood cell count and mean corpuscular volume were significantly (P<0.05) higher in infected cattle, while, red blood cells, packed cell volume hemoglobin concentration, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration (MCHC) were significantly (P<0.05) higher in non-infected as compare to infected animals.

Key words: Anaplasma marginale; cattle breeds; enzyme linked immuno sorbent assay; real-time polymerase chain reaction; transplacental transmission



Povzetek: Goveja anaplazmoza, ki jo povzroÄa Anaplasma marginale, je bolezen, ki ni kužna in jo prenaÅ¡ajo klopi. Glavni namen Å¡tudije je bil raziskati in primerjati pogostost anaplazmoze pri razliÄnih pasmah goveda in sicer dveh evropskih pasmah (holstein-frizijske pasma in pasma jersey) pri avtohtoni pasmi ter pri križancih med evropskimi pasmami in avtohtonimi pasmami (1. In 2. generacija križancev) ter prenos preko posteljice z uporabo verižne rekacije s polimerazo v realnem Äasu (PCR). Od skupno 96 pregledanih vzorcev krvi so rezultati pokazali skupno incidenco 45,83 % (44/96) A. marginale z najveÄjo incidenco 62,5 % (20/32) pri evropskih pasmah, nato 42,4 % (14/33) pri križanih živalih in 35,4 % (11/31) pri avtohtonih pasmah. Rezultati so tudi pokazali pozitivno rekacijo na prisotnost A. marginale pri 13,7 % (4/29) telet, kar kaže na prenos anaplazmoze preko posteljice. Metoda posrednega testa ELISA, ki pokaže na trenutno ali predhodno izpostavljenost, je pokazala 34,3-odstotno pojavnost pri vseh živalih skupaj (33/96 živali). ProuÄitev rdeÄih krvniÄk z barvanjem Giemsa je pokazalo 15 % (15/96) pozitivnih živali. StatistiÄna analiza je pokazala statistiÄno znaÄilno razliko v pojavnosti anaplazmoze med evropskima pasmama v primerjavi s križanci in avtohtono pasmo (p < 0,05), razlika med križanci in avtohtono pasmo goveda pa ni bila statistiÄno znaÄilna. Poleg tega je bil pri pojavnosti A. marginale opazen neznaten (statistiÄno neznaÄilen) uÄinek starosti prouÄevanih živali. Å tevilo belih krvnih celic in povpreÄni volumen telesne mase sta bila pri okuženih govedih statistiÄno znaÄilno poveÄana (p < 0,05), medtem ko so bile rdeÄe krvne celice, koncentracija hemoglobina v celicah in povpreÄna koliÄina hemoglobina v posameznem eritrocitu (MCHC) v neokuženi skupini viÅ¡ja kot v skupini z okuženimi živalmi (p <0,05).

KljuÄne besede: Anaplasma marginale; pasme goveda; test ELISA; verižna reakcija s polimerazo v realnem Äasu; prenos preko posteljice

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DOI: https://doi.org/10.26873/SVR-499-2018


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