SWINE BRUCELLOSIS CAUSED BY Brucella suis BIOVAR 2 IN CROATIA
DOI:
https://doi.org/10.26873/SVR-360-2017Abstract
Brucellosis in swine was surveyed from 2011 to 2015 in 13 counties in Croatia. A total of 3230 breeding males were tested serologically, and positive reactions were confirmed in 42 (1.3%) males from 17 farms. A total of 641 sows with abortion or reproductive problems were tested, and positive reactions were confirmed in 34 (5.3%). Organs from 68 swine were tested for bacteria, and Brucella spp. was isolated from 47 (69.1%). B. suis was identified in 45 isolates from domestic swine and 2 isolates from wild boar in six counties in Croatia, and all isolates were found to be B. suis biovar 2 based on Bru-up/Bru-low, Bruce-ladder, Suis-ladder and RFLP-based PCR assays. These results indicate that brucellosis is difficult to eradicate in free-range and semi-free-range swine farming, particularly in areas where contact with wild boar is possible. Further disease control measures are required.
Key words: Brucella suis biovar 2; swine; abortion; prevalence; Croatia
BRUCELOZA PRAŠIČEV, POVZROČENA Z BAKTERIJO Brucella suis BIOVAR 2 NA HRVAŠKEM
Brucelozo pri prašičih smo spremljali od leta 2011 do leta 2015 v 13 hrvaških občinah. S serološko analizo smo preverili prisotnost bruceloze pri 3230 samcih v razplodu. Pozitivne reakcije smo ugotovili pri skupno 42 merjascih s 17 farm, kar predstavlja 1,3 % živali. S serološkimi testi smo preverili prisotnost protiteles proti bruceli tudi pri 641 plemenskih svinjah, ki so zvrgle ali imele težave z zabrejitvijo. Pozitivna reakcija je bila ugotovljena pri 34 svinjah, kar predstavlja 5,3 % vseh testiranih živali. Notranje organe 68 svinj iz šestih občin smo uporabili za osamitev bakterij Brucella spp. Bakterije smo ugotovili pri 47 vzorcih (69,1 %). Bakterijo Brucella suis smo odkrili v vseh 47 vzorcih, izmed katerih jih je bilo 45 od domačih plemenskih svinj, dva vzorca pa sta bila od divjih svinj. Vse izolirane bakterije so pripadale sevu B. suis biovar 2, kot so pokazale dodatne analize z uporabo metod Bruup/Bru-low, Bruce-ladder, Suis-ladder in RFLP. Ti rezultati kažejo, da je popolno izkoreninjenje bruceloze težavno, še posebej v prostih rejah prašičev, kjer obstajajo možnosti stika z divjimi prašiči. Zato bi bilo v prihodnje potrebno razmisliti o dodatnih načinih nadzora nad to nevarno boleznijo prašičev.
Ključne besede: Brucella suis biovar 2; prašiči; zvrg; pojavnost; Hrvaška
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