ASSOCIATION OF NUMBER OF ARTIFICIAL INSEMINATIONS PER PREGNANCY IN HOLSTEIN DAIRY COWS WITH POLYMORPHISM IN LUTEINIZING HORMONE RECEPTOR AND FOLLICLE STIMULATING HORMONE RECEPTOR GENES

Authors

Abstract

Failure to become pregnant is the primary reason for a dairy cow to be culled from the production herd. A cow that is cycling normally, with no reproductive abnormalities, but has failed to conceive after at least three successive inseminations may cause economic losses in dairy farms. The present study aimed to examine the association between follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHCGR) genes polymorphisms and number of artificial inseminations in the Holstein cattle breed, raised in Turkey. A total of 264 Holstein cows were included in this study, consisting of 222 cows which had undergone a low number of artificial inseminations (two or less inseminations; LI) and 42 cows with a high number of artificial inseminations (three or more inseminations; HI). The polymerase chain reaction followed by restriction fragment length polymorphism method was used to determine the FSHR-AluI and LHCGR-HhaI DNA variants. Three genotypes (CC, CG and GG) were observed for the FSHR gene in LI and HI cows. No statistical difference was found among LI and HI animals for the FSHR genotypes (P=0.934).  However, only the CC genotype was detected in LI cows whereas the CC, CT and TT genotypes were detected in HI cows for the LHCGR gene. The genotype frequency of CC was found to be highest (93%) in the HI animals and an association between LHCGR genotypes and the number of artificial inseminations per pregnancy was identified (P<0.001). This is the first report to describes an association between FSHR and LHCGR polymorphisms and number of artificial inseminations in cows.

Key words: candidate genes; pregnancy; cow; number of artificial inseminations; polymorphism

 

POVEZAVA MED ŠTEVILOM UMETNIH OSEMENITEV IN  POLIMORFIZMOM GENOV ZA RECEPTOR ZA LUTEINIZIRAJOČI HORMON IN RECEPTOR ZA FOLIKLE STIMULIRAJOČI HORMON PRI KRAVAH MOLZNICAH PASME HOLSTEIN 

Težave z obrejitvijo so glavni razlog za izločitev krav molznic iz proizvodne črede. Krave z normalnim ciklusom, brez reproduktivnih motenj, ki se ne obrejijo po vsaj treh zaporednih osemenitvah, so vzrok za velike ekonomske izgube na mlečnih farmah. V opisani raziskavi smo proučevali povezavo med genskimi polimorfizmi v genih za receptorje za folikle stimulirajoči hormon (FSH-R), genih za receptorje za luteinizirajoči hormon (LH-R) ter številom umetnih osemenitev pri kravah pasme hlstein, vzrejenih v Turčiji. V raziskavo je bilo vključeno skupno 264 krav holstein, od katerih jih je bilo 22, osemenjenih največ dvakrat do obrejitve (skupina LI), in 42 krav, ki so bile osemenjene trikrat ali večkrat (skupina HI). Preiskovane gene smo pomnožili v verižni reakciji s polimerazo in nato izvedli pregled dolžine razrezanih odsekov DNK ( metoda RFLP) z namenom, da bi določili prisotnost različic genov FSHR-AluI in LHR-HhaI pri preiskovanih živalih. Tri različice genotipa (CC, CG in GG) so bile ugotovljene pri genu za FHSR pri kravah v skupinah LI in HI, med skupinama pa ni bilo statistično značilnih razlik v pogostnosti posameznih genotipov (p = 0,934). Pri genu za LHR je bil ugotovljen genotip CC le pri kravah iz skupine LI, ostale tri variante genotipa (CC, CT in TT) pa so bile ugotovljene pri kravah iz skupine HI.  Pogostnost genotipa CC je bila najvišja (93 %) pri živalih iz skupine HI, pri statistični analizi pa smo ugotovili povezavo med genotipi LH-R in številom umetnih osemenitev (p <0,001).

Ključne besede: FSH-R, LH-R; obrejitev; krava; število umetnih osemenitev; polimorfizem

Author Biography

Korhan Arslan, erciyes university

faculty of veterinary medicine

References

(1) Weaver LD. Reproductive management programs for large dairies. In: Morrow DA, ed. Current therapy in theriogenology. Philadelphia : W.B. Saunders Company, 1986: 383–9.

(2) Fetrow J, Stewart S, Eicker S. Reproductive health programs for dairy herds: analysis of records for assessment of reproductive performance. In: Youngquist RS, ed. Current therapy in large animal theriogenology. Philadelphia : W.B. Saunders Company, 1997: 441–51.

(3) Alaçam E. Inekte infertilite sorunu. In: Alaçam E, ed. Evcil Hayvanlarda Doğum ve Infertilite. Ankara: Medisan, 2007: 267–90.

(4) Olori VE, Meuwissen THE, Veerkamp RF. Calving interval and survival breeding values as measure of cow fertility in a pasture-based production system with seasonal calving. J Dairy Sci 2002; 85(3): 689–96.

(5) Bagnato A, Oltenacu PA. Phenotypic evaluation of fertility traits and their association with milk production of Italian Friesian cattle. J Dairy Sci 1994; 77(3): 874–82.

(6) Philipsson J. Genetic aspects of female fertility in dairy cattle. Livest Prod Sci 1981; 8(4): 307–19.

(7) Colleau JJ, Moureaux S. Constructing the selection objective of the French Holstein population. Interbull Bull 1999; 23: e41 (5 pp.). https://journal.interbull.org/index.php/ib/article/view/379/379 (July, 2016)

(8) Esslemont RJ, Kossaibati MA, Allcock J. Economics of fertility in dairy cows. In: Fertility in the high producing dairy cow. Br Soc Anim Sci Occasional Publ 2001; 26: 21–9.

(9) Akyuz B, Sariozkan S, Bayram D. Factor XI mutation in normally fertile and repeat breeding Holstein cows in the Middle Anatolian region of Turkey: a financial approach. Anim Prod Sci 2012; 52(11): 1042–5.

(10) Pryce JE, Esslemont RJ, Thompson R, et al. Estimation of genetic parameters using health, fertility and production data from a management recording system for dairy cattle. Anim Sci 1998; 66(3): 577–84.

(11) Walsh SW, Williams EJ, Evans ACO. A review of the causes of poor fertility in high milk producing dairy cows. Anim Reprod Sci 2011; 123: 127–38.

(12) Washburn SP, Silvia WJ, Brown CH, et al. Trends in reproductive performance in southeastern Holstein and Jersey DHI herds. J Dairy Sci 2002; 85(1): 244–51.

(13) Lucy MC, Billings HJ, Butler WR, et al. Efficacy of an intravaginal progesterone insert and an injection of PGF2 alpha for synchronizing estrus and shortening the interval to pregnancy in postpartum beef cows, prepubertal beef heifers, and dairy heifer. J Anim Sci 2001; 79(4): 982–95.

(14) Tiezzi F, Pretto D, De Marchi M, et al. Heritability and repeatability of milk coagulation properties predicted by mid-infrared spectroscopy during routine data recording, and their relationships with milk yield and quality traits. Animal 2013; 7(10): 1592-9.

(15) Höglund JK, Sahana G, Guldbrandtsen B, et al. Validation of associations for female fertility traits in Nordic Holstein, Nordic Red and Jersey dairy cattle. BMC Genet 2014; 15: e8 (7 pp.) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898023/pdf/1471-2156-15-8.pdf (July, 2016)

(16) Pimentel ECG, Bauersachs S, Tietze M, et al. Exploration of relationships between production and fertility traits in dairy cattle via association studies of SNPs within candidate genes derived by expression profiling. Anim Genet 2011; 42(3): 251–62.

(17) Giesecke K, Hamann H, Sieme H, et al. INHBA-associated markers as candidates for stallion fertility. Reprod Domest Anim 2010; 45(2): 342–7.

(18) Othman OE, Abdel-Samad MF. RFLP polymorphism of three fertility genes in Egyptian buffalo. J Appl Bio Sci 2013; 7(2): 94–101.

(19) Mayorga MP, Gromoll J, Behre HM, et al. Ovarian response to follicle-stimulating hormone (FSH) stimulation depends on the FSH receptor genotype. J Clin Endocr Metab 2000; 85(9): 3365–9.

(20) Houde A, Lambert A, Saumande J, et al. Structure of the bovine follicle-stimulating hormone receptor complementary DNA and expression in bovine tissues. Mol Reprod Dev 1994; 39(2): 127–35.

(21) Yang W, Tang K, Li S, et al. Polymorphisms of the bovine luteinizing hormone/choriogonadotropin receptor (LHCGR) gene and its association with superovulation traits. Mol Biol Rep 2012; 39(3): 2481–7.

(22) Zimin AV, Delcher AL, Florea L, et al. A whole-genome assembly of the domestic cow, Bos taurus. Genome Biol 2009; 10(4): eR42 (10 pp.) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688933/pdf/gb-2009-10-4-r42.pdf (July, 2016)

(23) Yu Y, Pang Y, Zhao H, et al. Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers. J Anim Sci Biotechnol 2012; 3(1): e35 (5 pp.) https://jasbsci.biomedcentral.com/articles/10.1186/2049-1891-3-35 (July, 2016)

(24) Bassiouny YA, Rabie WA, Hassan AA, et al. Association of the luteinizing hormone/choriogonadotropin receptor gene polymorphism with polycystic ovary syndrome. Gynecol Endocrınol 2014; 30(6): 428–30.

(25) NRC. Nutrient requirements of dairy cattle 2001. 7th ed. Washington : National Research Council, National Academy Press, 2001: 15–8.

(26) Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor : Cold Spring Harbor Laboratory Press, 1989.

(27) Price JE, Royal MD, Garnsworthy PC, et al. Fertility in high producing dairy cows. Livest Prod Sci 2004; 86: 125–35.

(28) Yang WLS, Tang K, Hua G, et al. Polymorphisms in the 5′ upstream region of the FSH receptor gene, and their association with superovulation traits in Chinese Holstein cows. Anim Reprod Sci 2010; 119: 172–7.

(29) Marson EP, Ferraz JBS, Meirelles FV, et al. Genetic characterization of European-Zebu composite bovine using RFLP markers. Genet Mol Res 2005; 4(3): 496–505.

(30) Marson EP, Ferraz JBS, Meirelles FV, Balieiro JCC, Eler JP. Effects of polymorphisms of LHR and FSHR genes on sexual precocity in a Bos taurus x Bos indicus beef composite population. Genet Mol Res 2008; 7(1): 243–51

(31) Cory AT, Price CA, Lefebvre R, et al. Identification of single nucleotide polymorphisms in the bovine follicle-stimulating hormone receptor and effects of genotypes on superovulatory response traits Anim Genet 2012; 44(2): 197–201.

(32) Sudo S, Kudo M, Wada S, et al. Genetic and functional analyses of polymorphisms in the human FSH receptor gene. Mol Hum Reprod 2002; 8(10): 893–9.

(33) De Castro F, Ruiz R, Montoro L, et al. Role of follicle-stimulating hormone receptor Ser680Asn polymorphism in the efficacy of follicle-stimulating hormone Fertil Steril 2003; 80(3): 571–6.

(34) Bage R, Gustafson H, Larsson B, et al. Repeat breeding in dairy heifers: follicular dynamics and estrous cycle characteristics in relation to sexuan hormone patterns. Theriogenology 2002; 57(9): 2257–69.

(35) Milazzotto MP. Mutações no gene do receptor do hormônio luteinizante (LHR) bovino e associação com precocidade sexual em fêmeas Bos primigenius indicus (Nelore): M.Sc. thesis. Botucatu : Campus de Botucatu, Instituto de Biociências, Universidade Estadual Paulista, Brazil, 2001.

(36) Holmberg M, Andersson-Eklund L.Quantitative trait loci affecting fertility and calving traits in Swedish dairy cattle. J Daıry Sci 2006; 89(9): 3664–71.

(37) Hu ZL, Park CA, Reecy JM. Developmental progress and current status of the Animal QTLdb. Nucleıc Acıds Res 2015; 44(1): 827–33.

Downloads

Published

2017-07-08

How to Cite

Arslan, K., Akyüz, B., Akçay, A., İlgar, E. G., Macun, H. C., & Çınar, M. U. (2017). ASSOCIATION OF NUMBER OF ARTIFICIAL INSEMINATIONS PER PREGNANCY IN HOLSTEIN DAIRY COWS WITH POLYMORPHISM IN LUTEINIZING HORMONE RECEPTOR AND FOLLICLE STIMULATING HORMONE RECEPTOR GENES. Slovenian Veterinary Research, 54(2). Retrieved from https://slovetres.si/index.php/SVR/article/view/274

Issue

Section

Original Research Article