Ines Varga, Nina Bilandzic, Mario Mitak, Luka Cvetnić, Ivana Varenina, Solomun Kolanović Božica, Božić Luburić Đurđica, Marija Perković, Željko Cvetnić


The aim of this study was to monitor the biochemical and chemical parameters of the blood of commercial chickens following treatment with one of three coccidiostats: maduramycin, monensin or diclazuril. Chickens received feed treated with maduramycin at concentrations of 5, 10 and 15 mg kg-1, monensin at 125, 225 and 325 mg kg-1 or diclazuril at 1, 5 and 10 mg kg-1. A control group of chickens consumed feed without the addition of coccidiostats. Following treatment, blood was sampled for 11 days and analysed for the following biochemical and chemical parameters: aspartate aminotransferase (AST), bile acid (BA), creatine kinase (CK), uric acid (UA), glucose (GLU), cholic acid (CA), total protein (TP), albumin (ALB), globulin (GLOB) and phosphorus (PHOS). Administration of different concentrations of maduramycin, monensin and diclazuril did not affect the concentration of the parameters AST, UA, GLU, BA, TP, ALB, GLOB and PHOS in experimental groups of broilers in relation to the control group. However, significant differences were observed in the concentrations of CK and CA between the experimental and control groups. Significant differences were also found in the concentrations of AST, CA and UA between experimental groups.

Key words: maduramycin; monensin; diclazuril; chickens; biochemical and chemical parameters



Namen raziskave je bil spremljanje biokemijskih in kemijskih parametrov krvi industrijsko gojenih piščancev po zdravljenju z enim od treh kokcidiostatikov: maduramicinom, monenzinom ali diklazurilom. Piščanci so dobivali krmo z maduramicinom v koncentracijah 5, 10 in 15 mg/kg, monenzin v koncentracijah 125, 225 in 325 mg/kg ali diklazuril v koncentracijah 1, 5 in 10 mg/kg. Kontrolna skupina piščancev je dobila krmo brez dodatka kokcidiostatikov. Enajsti dan po zdravljenju je bila piščancem odvzeta kri in analizirani so bili naslednji biokemijski in kemijski parametri: koncentracija aspartatne aminotransferaze (AST), žolčnih kislin (BA), kreatininske kinaze (CK), sečne kisline (UA), glukoze (Glu), skupnih beljakovin (TP), albuminov (ALB), globulinov (GLOB) in fosforja (Phos). Dodajanje različnih koncentracij maduramicina, monenzina in diklazurila ni vplivalo na koncentracijo parametrov AST, UA, Glu, BA, TP, ALB, GLOB in Phos v poskusnih skupinah pitovnih piščancev v primerjavi s kontrolno skupino. Statistično značilne razlike so bile ugotovljene v koncentracijah CK in CA med poskusnimi in kontrolno skupino. Značilne razlike so bile ugotovljene tudi v koncentracijah AST, CA in UA med posameznimi poskusnimi skupinami.

Ključne besede: maduramicin; monenzin; diklazuril; piščanci; biokemijski in kemijski parametri

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(1) Peek H. Resistance to anticoccidial drugs: alternative strategies to control coccidiosis in broilers: doctoral thesis Utrecht : Faculty of Veterinary Medicine, University of Utrecht, 2010.

(2) Chapman HD, Jeffers TK. Vaccination of chickens against coccidiosis ameliorates drug resistance in commercial poultry production. Int J Parasitol 2014; 4: 214–7.

(3) EC. Report from the Commission to the council and the European Parliament on the use of coccidiostats and histomonostats as feed additives submitted pursuant to article 11 of regulation (ec) no 1831/2003 of the European Parliament and of the Council of 22 september 2003 on additives for use in animal nutrition. Commission of the European Communities, COM(2008)233 final. Brussels : EC, 2008.

(4) Rutkowski J, Brzezinski B. Structures and properties of naturally occurring polyether antibiotics. BioMed Res Int 2013; 2013: e162513 (31 pp.) (April 2016)

(5) Dorne JLCM, Fernández-Cruz ML, Bertelsen U, et al. Risk assessment of coccidostatics during feed cross-contamination: animal and human health aspects. Toxicol Appl Pharmacol 2013; 270: 196–208.

(6) Chapman HD, Cherry TE, Danforth HD, Richards G, Shirley MW, Williams RB. Sustainable coccidiosis control in poultry production: the role of live vaccines. Int J Parasitol 2002; 32: 617–29.

(7) Rokka M, Jestoi M, Peltonen K. Trace level determination of polyether ionophores in feed. BioMed Res Int 2013; 2013: e151363 (12 pp.) (April 2016)

(8) Markiewicz W, Barski D, Tomaszewska E, Burmańczuk A. Toxicity of salinomycin and narasin in turkeys. J Elementol 2014; 19: 903–14.

(9) Sharma N, Bhalla A, Varma S, Jain S, Singh S. Toxicity of maduramycin. Emerg Med J 2005; 22: 880–2.

(10) Varenina I, Bilandžić N, Cvetnić L et al. Deposition and depletion of maduramycin residues in eggs after oral administration to laying hens determined by LC-MS. Food Addit Contam A 2015; 32: 324-32.

(11) EC. Commission implementing Regulation (EU) No 388/2011 of 19 April 2011 concerning the authorisation of maduramicin ammonium alpha as a feed additive for chickens for fattening (holder of authorisation Alpharma (Belgium) BVBA) and amending Regulation (EC) No 2430/1999. Off J Eur Commun 2011; L 104: 3–6.

(12) Chapman HD, Jeffers TK, Williams RB. Forty years of monensin for the control of coccidiosis in poultry. Poultry Sci 2010; 89: 1788–801.

(13) EC. Commission implementing Regulation (EU) No 495/2011 of 20 May 2011 amending Regulation (EC) No 109/2007 as regards the composition of the feed additive monensin sodium. Off J Eur Commun 2011; L 134: 6–8.

(14) EC. Commission Regulation (EC) No 976/2008 of 6 October 2008 amending Regulations (EC) No 2430/1999, (EC) No 418/2001 and (EC) No 162/2003 as regards the terms of the authorisation of the feed additive ‘Clinacox’, belonging to the group of coccidiostats and other medicinal substances. Off J Eur Commun 2008; L 266: 3–7.

(15) EC. Commission implementing Regulation (EU) No 1235/2013 of 2 December 2013 amending the Annex to Regulation (EU) No 37/2010 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin, as regards the substance diclazuril. Off J Eur Commun 2013; L 322: 21–22.

(16) Andreasen JR, Andreasen CB, Sonn AB, Robeson DC. The effects of haemolysis on serum chemistry measurements in poultry. Avian Pathol 1996; 25: 519–36.

(17) Elsayed M, Elkomy A, Aboubakr M, Morad M. Tissue residues, hematological and biochemical effects of tilmicosin in broiler chicken. Vet Med Int 2014; 2014: e502872 (6 pp.) (May, 2016)

(18) Harvey JW. Veterinary hematology: a diagnostic guide and color atlas. St. Louis : Elsevier/Saunders, 2012: 2.

(19) Albokhadaim I, Althnaian T, EL-Bahr SM. Investigation of selected biochemical parameters of local chickens with different age and sex in Al-ahsa, Saudia Arabia. Pakistan J Biol Sci 2012; 15: 827–32.

(20) EFSA. Cross-contamination of non-target feedingstuffs by diclazuril authorised for use as a feed additive. EFSA J 2008; 716: 2–31.

(21) Harr KE. Diagnostic value of biochemistry. In: Harrison GJ, Lightfoot T, eds. Clinical avian medicine. Volume II. Palm Beach : Spix Publishing, 2006: 611–30.

(22) Thrall MA, Weiser G, Allison RW, Campbell TW. Veterinary hematology and clinical chemistry. 2nd ed. Danvers : Wiley-Blackwell, 2012.

(23) Deljou M, Reza Aslani M, Mohri M, Reza Movassaghi A, Heidarpour M. Clinical, laboratory and pathological findings in sub-acute monensin intoxication in goats. Vet Res Forum 2014; 5: 161–67.

(24) Hematological and serum biochemical reference guides. In: Cynthia MK, ed. Merck veterinary manual. 10th ed. Kenilworth, NJ : Merck Sharp & Dohme Carp, 2011.

(25) Karabacak M, Çinar M, Eraslan G, Kanbur M, Liman BC, Akçay A. The effects of grape seed on some biochemical parameters in ionophore antibiotics treated broiler. Erciyes Üniv Vet Fak Derg 2015; 12: 37–41.

(26) Arun KHS, Manjunath HS, Reddy KS, Reddy R, Kalakumar B. Effect of subacute doses of maduramycin in broiler chicks. Online J Vet Res 2003; 7: 81–8.

(27) Khan MZ, Szarek J, Koncicki A, Krasnodębska-Depta A. Oral administration of monensin and lead to broiler chicks: effects on haematological and biochemical parameters. Acta Vet Hung 1994; 42: 111–20.

(28) Shlosberg A, Harmelin A, Perl S, et al. Cardiomyopathy in cattle induced by residues of the coccidiostat maduramycin in poultry litter given as a feedstuff. Vet Res Commun 1992; 16: 45–58.

(29) Stamp D, Jenkins G. An overview of bile-acid synthesis, chemistry and function. In: Jenkins GJ, Hardie LJ, eds. Bile acids: toxicology and bioactivity. Cambridge, UK : Royal Society of Chemistry, 2008: 1–13.

(30) Fuller MF. The encyclopedia of farm animal nutrition.Wallingford : CABI Publishing, 2004.

(31) Patra G, Ayub Ali M, Victoria Chanu KH, et al. PCR based diagnosis of Eimeria tenella infection in broiler chicken. Int J Poultry Sci 2010; 9: 813–8.

(32) Hochleithner M. Biochemistries. In: Ritchie BW, Harrison GJ, Harrison LR, eds. Avian medicine: principles and application. Lake Worth, Florida, : Wingers Publishing, 1994: 223–45.

(33) Talebi A. Biochemical parameters in broiler chickens vaccinated against ND, IB and IBD. Int J Poultry Sci 2006; 5 (12): 1151–5.

(34 Stabel JR, Harvey JW, Locas DM. Salinomycin and lasalocid effects on growth performance, mineral metabolism and ruminal fermentation in steers. J Anim Sci 1989; 67: 735–42.

(35) Hussein MSH, Abd-El-Rahman AH. Hematological, biochemical, immunological and histopathological changes caused by salinomycin changes caused by salinomycin in chicken. Egyptian J Natur Tox 2005; 2: 13–38.


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