EFFECTS OF ISCHEMIA AND REPERFUSION INDUCED BY UTERINE PROLAPSE ON CARDIAC BIOMARKERS IN SHEEP
Effects of Uterine Prolapse on Cardiac Biomarkers in Sheep
DOI:
https://doi.org/10.26873/SVR-2325-2026Keywords:
cardiac biomarker, ischemia, reperfusion, sheep, uterine prolapseAbstract
The aim of this study was to determine the cardiac damage caused by uterine prolapse in sheep, to reveal the cardiac changes that occur depending on the time elapsed since the event, and to evaluate the ischemia-reperfusion (I/R) injury induced by reperfusion following treatment using a prolapsed uterine model. The animal material of the study consisted of a total of 35 Awassi sheep that had given birth at least once and were within the first 24 hours postpartum. The sheep were divided into five groups according to the mode of delivery and the time of onset of uterine prolapse. The control group (Group 1, n=7) consisted of sheep that gave birth normally without any intervention. The groups that developed uterine prolapse were as follows: The study consisted of sheep that developed prolapse within 0-6 hours (Group 2, n=7), 6-12 hours (Group 3, n=7), 12-18 hours (Group 4, n=7), and 18-24 hours (Group 5, n=7) depending on the duration of prolapse. Cardiac troponin (cTnI), creatine kinase myocardial band (CK-MB), superoxide dismutase (SOD), catalase (CAT), malondealdehyde (MDA), interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-α) levels were determined using a commercial ELISA kit from blood samples taken within one hour after birth from the sheep in the control group and before and 4 hours after treatment from the prolapsed uteri groups. Evaluations performed before and after prolapse treatment revealed significant differences both within and between groups in heart rate, respiratory rate, body temperature, WBC, cTnI, CK-MB, AST (lactate dehydrogenase), LDH, SOD, CAT, MDA, IL-6, and TNF-α (P<0.001). In conclusion, uterine prolapse causes serious cardiac and systemic effects in sheep, exacerbating ischemia-reperfusion injury. Therefore, early diagnosis and rapid intervention are critical for minimizing cardiac damage.
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