SEX-BASED DIFFERENCES IN GROSS MORPHOMETRY AND MICROSCOPY OF THE PANCREAS OF JAPANESE QUAIL (Coturnix japonica)
DOI:
https://doi.org/10.26873/SVR-2276-2026Keywords:
endocrine and exocrine pancreas, histomorphometry, Japanese quail, pancreatic ducts, sex differencesAbstract
The pancreas is a very important organ in all mammals including birds, which is involved in digestion and metabolism, yet little is known about structural differences between male and female birds. Forty adult (20 males and 20 females) Japanese quails (Coturnix coturnix japonica) were used to analyze the morphometrical and histological differences between the pancreatic lobes. The gross anatomical results showed that both sexes have four lobes: dorsal, ventral, third, and small splenic lobe. Morphometric analysis revealed that the dorsal lobe was significantly heavier and wider in females than in males, suggesting possible sex-linked metabolic adaptations while differences in the dimensions of the ventral and third lobes were not significant. The exocrine tissue showed no differences between males and females, maintaining a consistent structure similar to other birds. However, the endocrine portion, particularly the number of islets of Langerhans, revealed sex-specific differences where females had significantly more islets in the dorsal lobe than the males, whereas males had slightly more but not significantly higher number of islets in the ventral lobe than females. Additionally, the pancreatic ductal system exhibited similar structural features in both sexes, starting from centroacinar cells to the main pancreatic duct with evidence of mucin production by the ductal epithelium observed throughout the ducts despite of the absence of the goblet cells. These findings highlight the variations in morphometry and histology of the quail pancreas between sexes, offering new perspectives on avian physiology and potential metabolic differences. This research lays the basis for further studies on the functional implications of these histomorphometrical distinctions in health and disease.
References
Mobini B. Histochemical and histological studies on the pancreas in mature pigeon (Columba Livia). Eur J Exp Biol 2013;3 (2):148-152.
Deprem T, Tasci SK, Bingol SA, et al. Histological and histochemical studies on the structure of pancreatic ducts of goose. Turk J Vet Anim Sci 2015;39:62-68.
Liu JW, Evans H, Larsen P, et al. Gross anatomy of the pancreatic lobes and ducts in six breeds of domestic ducks and six species of wild ducks in China. Anat Histol Embryol 1998;27:413- 417.
Saadatfar Z, Asadian M. Anatomy of Pancreas in Mynah (Acridotheres tristis). J Appl Anim Res 2009;36: 191-193.
Ku SK, Lee JH, Lee HS. An immunohistochemical study of the insulin-, glucagon- and somatostatin- immunoreactive cells in the developing pan-creas of the chicken embryo. Tissue Cell 2000;32:58-65.
Gülmez N. Are glands present in goose pancreatic ducts? A light mic-roscope study. J. Pancreas 2003;4:125-128.
Simsek N, Alabay B. (2008): Light and electron microscopic examinations of the pancreas in quails (Coturnix coturnix japonica). Revue Méd Vét 2008;159(4):198-206.
Simsek NI, Oziidogru Z, Alabay B. (2008): Immunohistochemical Studies on the Splenic Lobe of the Pancreas in Young Japanese Quails (Coturnix c. japonica). Deutsche Tierarztliche Wochenschrift 2008;115(5):189-193.
Helmy SA, Soliman MT. Histological, histochemical and ultrastructure studies on the Ostrich Pancreas (Struthio camelus). Egy Acad J Biol Sci Histol Histochem 2018;10 (1):63-77.
Hussein A, Bargooth AF. Comparative Histological Study of Exocrine Pancreas in Duck (Anas platyrhnchos) and Turkey (Meleagris gallopavo). Wasit J Pure Sci 2022;1(2):138-48.
Naser RA, Almaliki SH, Zghair FS, et al. Study of morphological and histo-logical properties of the pancreas in crow (Linnaecus corvus) and Iraqi black partridge (Melanoperdix niger). Open Vet J 2024;14(10):2634.
Saadatfar Z, Asadian M, Alishahi E. Structure of Pancreas in Zebra Finch (Poephila bichenovi) and Pigeon (Streptopelia decaocto laughing nove). J Appl Anim Res 2010:37:157-160.
Mobini B. (2011): Histological Studies on Pancreas of Goose (Anser Albifrons). Vet Res Forum 2011;2(1): 25-29.
Baumel JJ. Handbook of avian anatomy: nomina anatomica avium. Publications of the Nuttall Ornithological Club (USA). 1993, no. 23.
Kadhim KK, Zuki1 ABZ, Noordin MM, et al. Morphological study of pan-creatic duct in red jungle fowl. Afri J Biotech 2010;9(42):7209-7215.
Parchami A, Kusha S. Effect of sex on histomorphometric properties of Langerhans islets in native chickens. Vet Res Forum 2015;6(49):327.
Ibrahim MIA, Zakariah M, Molele RA, et al. Ontogeny of the testicular excurrent duct system of male Japanese quail (Coturnix japonica): a histolo-gical, ultrastructural, and histometric study. 2022; 85:1160-70.
Suvarna KS, Layton C, Bancroft JD. Bancroft's theory and practice of histological techniques. Elsevier health sciences; 2018.
Beheiry RR, Abdel-Raheem WA, Balah AM, et al. Morphological, histolo-gical and ultrastructural studies on the exocrine pancreas of goose. Beni-Suef Unive J Basic Appl Sci 2018;7(3):353-8.
Al-agele RAA, Mohammed FS. Architecture morphology and histological in- vestigations of pancreas in Golden Eagles (Aquila Chrysaetos). Al-Anbar J Vet Sci 2012;5(2):149-155.
Al-Khakani SA, Zabiba IJ, Al-Zubaidi K, et al. Morphometrical and histo-chemical foundation of the pancreas and ductal system in white eared bul-bul (Pycnonotus leucotis). Iraqi J Vet Sci 2019;33(1):99-104.
Tarakcy BG, Yaman M, Bayrakdar A. Immunohistochemical Study on the Endocrine Cells in the Pancreas of the Ostrich (Struthio camelus). J Anim Vet Adv 2007;6 (5):693-696.
Al-Sharoot HA. Anatomical, histological and histochemical architecture of pancreases in early hatched goose (Anser anser). Kufa J Vet Med Sci 2016;7(1):147-53.
Al-Haaik A. A gross anatomical and histological study of pancreas in adult Kestrel (Falco tinnunculus). Iraqi J of Vet Sci 2019;33(2):175-80.
Bailey TA, Mensah-Brown EP, Samour JH, et al. Comparative morphology of the alimentary tract and its glandular derivatives of captive bustards. J Anat 1997;3 (3):387-398.
Kara A, Tekiner D, Simsek N, et al. Distribution and location of endocrine cells in the pancreas of the sparrow hawk (Accipiter nisus). Kafka's Univ Vet Fak-Derg 2014;20 (2):307-312.
Mahmood SK, Ahmed NS, Sultan GA, et al. Histomorphological and carbohydrate histochemical study of the pancreas in native ducks (Anas Platyrhynchos). Iraqi Journal of Veterinary Sciences. 2022;36(4):1103-10.
Doneley RJ. Acute pancreatitis in parrots. Aust Vet J 2001;79 (6):409–411.
Stornelli MR, Ricciardi MP, Miragliotta V, et al. Morpho- structural study of the pancreas and pancreatic duct in ostrich (Struthio camelus). Acta Veterinaria Brno 75 (2), 157–160.
Suri S, Sasan JS, Khan A. Histo-morphometrical study on the pancreas of Turkey. Indian Journal of Veterinary Sciences and Biotechnology. 2022:19; 18(2):59-62.
Yehia OA, Ahmed YH, Elleithy EM, et al. Comparative histological, histo-chemical and ultrastructure studies on the exocrine pancreas of Japanese quail (Coturnix coturnix japonica) and cattle egret (Bubulcus ibis). Int J Vet Sci 2021;10(2):107-113.
Das A, Das RK, Parida S, et al. Histomorphological Study on pancreas of duck. Ind J Anim Sci 2003;73(6):598-599.
Beheiry RR, Salem HF, Karkit MW. Ultrastructural studies on the pancrea-tic acini in duck (Anas boscas) and pigeon (Columba livia). Anatomia Histolo-gia Embryologia 2020;49(3):345-50.
Mobini B, Aghaabedi B. Histological and histochemical studies on pan-creas of native turkey in Iran. Vet J Pajouhesh Sazandegi 2009;22:2–8.
Umar Z, Qureshi AS, Shahid RU, et al. Macroscopic, Microscopic and Histomorphometric Analysis of Intestine, Liver and Pancreas of Ostrich (Struthio camelus) with Advancement of Age and Sex. Pakistan Vet J 2021;41(3):313-320.
Atia GM., D.R. El-Bassouny. Pancreatic stellate cells and alcohol induced chronic pancreatitis in albino rats (Histological and immunohistochemical study) Egypt J. Histol., 33 (4) (2010), pp. 757-766.
Al-boudi AS, Ibraheem E. Grossly and microscopical description of duck's pancreas. Al-Qadisiyah J Vet Med 2005;1(4):1-5.
Saadatfar Z, Asadian M, Alishahi E. Structure of pancreas in Palam Dove (Streptoplia selegalensis). Iran J Vet Sci Tech 2011;3(2):25-32.
Motta MP, Macchiarelli G, Nottola SA. Histology of the exocrine pancre-as. Microsc Res Tech 1997;37:384-398.
Mikami SI, Taniguchi K, Ishikawa T. Immunocytochemical localization of the pancreatic islet cells in the Japanese quail, Coturnix coturnix japonica. Jpn J Vet Sci 1985;47(3): 357-369.
Fatahian Dehkordi RA, Moradi H. Stereological estimation and morpho-logical assessment of the endocrine pancreatic components in relation to sex in hen. Vet Res Forum 2015;6(1):49-54.
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