LIP RECONSTRUCTION, İNTRAORAL BARRIER AND AUTOLOGOUS PLATELET RICH PLASMA (PRP) APPLICATION IN A DOG WITH LIP NECROSIS DUE TO SNAKE BITE
New Methods Contributing to Flap Vitality
DOI:
https://doi.org/10.26873/SVR-2222-2025Keywords:
dog, flap operation, large tissue lost, lip reconstriction, PRP, interdental siliconeAbstract
The study case consisted of a 2-year-old male Jack Russell mixed breed dog with a delayed wound due to snake bite. The necrosis detected on clinical examination in the area starting from the left maxillary and mandibular canine teeth level to the anterior part of the ear base, the upper and lower lips including the left maxillary and mandibular teeth, and the cheek skin, with tissue loss. In the blood sample collected, platelets were recorded at 44 (x109 /L); however, the analysis of PRP revealed an approximately sixfold rise, with platelet levels reaching 240 (x109 /L). During the first 15 days, medical treatment was used to prepare the wound for surgery. Caudal Auricular Axial Pattern Flap technique was used in the first operation, and Pedicle Advancement Flap technique was used in the second operation. After both operations, dressings with ointments and systemic treatment continued. At the end of first operation, as a result of the large donor tissue area and the resulting inadequate nutrition and the mechanical irritation created by the patient's tongue, tissue rejection and necrosis occurred at the tip of the flap applied to the lower jaw at the level of the mandibulary canine tooth, leading to loss of flap tissue. Therefore, to prevent tissue necrosis, accelerate flap acceptance and ensure flap vitality, platelet-rich plasma (PRP) was applied one week postoperatively. It was concluded that PRP is a usefull method that accelerate angiogenesis, epithelialization, acceptance of the flap and to prevent flap necrosis.
References
Chung TH, Baek DS, Kim N, Park JH, Park C. Topical allogeneic platelet-rich plasma treatment for a massive cutaneous lesion induced by disseminated intravascular coagulation in a toy breed dog. Ir Vet J 2015; 68: 1-4. doi: 10.1186/s13620-015-0032-7
Farghali HA, AbdElKader NA, Khattab MS, AbuBakr HO. Evaluation of sub-cutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs. Biosci Rep 2017; 37(2): BSR20160503. doi: 10.1042/BSR20160503.
Muthuprabakaran K, Pai VV, Ahmad S, Shukla P. A cross-sectional analysis of the effects of various centrifugation speeds and inclusion of the buffy coat in platelet-rich plasma preparation. IJDVL 2021; 87 (6): 792-99. doi:10.25259/IJDVL_1050_20.
Perinelli DR, Bonacucina G, Pucciarelli S, et al. Rheological properties and growth factors content of platelet-rich plasma: relevance in veterinary biomedical treatments. Biomed 2020; 8(10): 429. doi: https://doi.org/10.3390/biomedicines8100429.
Anitua E, Sánchez M, Orive G, Andía I. The potential impact of the prepa-ration rich in growth factors (PRGF) in different medical fields. Biomaterials 2007; 28(31): 4551-60. doi: https://doi.org/10.1016/j.biomaterials.2007.06.037
Bennett NT, Schultz GS. Growth factors and wound healing: part II. Role in normal and chronic wound healing. Am J Surg 1993; 166: 74-81. doi: 10.1016/S0002-9610(05)80589-6.
Borzini P, Mazzucco L. Tissue regeneration and in loco administration of platelet derivatives: clinical outcome, heterogeneous products, and hetero-geneity of the effector mechanisms. Transfusion 2005; 45: 1759-67. doi: 10.1111/j.1537-2995.2005.00600.
Sardari K, Emami MR, Kazemi H, et al. Effects of platelet-rich plasma (PRP) on cutaneous regeneration and wound healing in dogs treated with dexamethasone. Comp Clin Path 2010; 20: 155-62. doi: 10.1007/s00580-010-0972-y.
Tambella AM, Attili AR, Dupré G, et al. Platelet-rich plasma to treat expe-rimentally-induced skin wounds in animals: a systematic review and meta-analysis. PLoS one 2018; 13(1): e0191093. doi: 10.1371/journal.pone.0191093.
Hussein SM. Effects of autologous platelet-rich plasma on skin healing in dogs. Iraqi J Vet Sci 2018; 32: 275-283. doi: 10.33899/ijvs.2019.153864.
Jee CH, Eom NY, Jang HM, et al. Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs. J Vet Sci 2016; 17: 79-87. doi: 10.4142/jvs.2016.17.1.79.
Karayannopoulou M, Psalla D, Kazakos G, et al. Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs. Vet Comp Orthop Traumatol 2015; 28: 172-78. doi: 10.3415/VCOT-14-06-0088.
Kim JH, Park C, Park HM. Curative effect of autologous platelet-rich plasma on a large cutaneous lesion in a dog. Vet Dermatol 2009; 20: 123-26. doi: 10.1111/j.1365-3164.2008.00711.
Tambella AM, Attili AR, Dini F, et al. Autologous platelet gel to treat chronic decubital ulcers: a randomized, blind controlled clinical trial in dogs. Vet Surg 2014; 43: 726-33. doi: 10.1111/j.1532-950X.2014.12148.x,
Pavletic MM. Facial Reconstruction. In: Pavletic MM, ed. Atlas of small animal wound management and reconstructive surgery. 4th Ed. John Wiley & Sons: 2018; 493-552.
Berni P, Leonardi F, Conti V, Ramoni R, Grolli S, Mattioli G. Case Report: A Novel Ventilated Thermoplastic Mesh Bandage for Post-operative Mana-gement of Large Soft Tissue Defects: A Case Series of Three Dogs Treated With Autologous Platelet Concentrates. Front Vet Sci 2021; 8: 704567. doi: 10.3389/fvets.2021.704567
Hasiba-Pappas SK, Tuca AC, Luze H, et al. Platelet-Rich Plasma in Plastic Surgery: A Systematic Review. Transfus Med Hemother 2022; 49(3): 129-42. doi: 10.1159/000524353.
Abouelnasr K, Hamed M, Lashen S, El-Adl M, Eltaysh R, Tagawa M. Enhan-cement of abdominal wall defect repair using allogenic platelet-rich plasma with commercial polyester/cotton fabric (Damour) in a canine model. J Vet Med Sci 2017; 79(7): 1301-09. doi: https://doi.org/10.1292/jvms.17-0139.
Iacopetti I, Patruno M, Melotti L, et al. Autologous platelet-rich plasma enhances the healing of large cutaneous wounds in dogs. Front Vet Sci 2020; 7: 575449. doi: 10.3389/fvets.2020.575449.
Zubin E, Conti V, Leonardi F, Zanichelli S, Ramoni R, Grolli S. Regenerative therapy for the management of a large skin wound in a dog. Clin Case Rep 2015; 3(7): 598-603. doi: 10.1002/ccr3.253.
Degner DA. Facial reconstructive surgery. Clin Tech Small Anim Pract 2007; 22(2): 82-8. doi: 10.1053/j.ctsap.2007.03.009
Field EJ, Kelly G, Pleuvry D, Demetriou J, Baines SJ. Indications, outcome and complications with axial pattern skin flaps in dogs and cats: 73 cases. J Small Anim Pract 2015; 56(12): 698-706. doi: 10.1111/jsap.12400.
Hermeto LC, Rossi RD, Pádua SB, Pontes ER, Santana AE. Comparative study between fibrin glue and platelet rich plasma in dogs skin grafts. Acta Cir Bras 2012; 27: 789-94. doi: https://doi.org/10.1590/S0102-86502012001100008.
Karayannopoulou M, Papazoglou LG, Loukopoulos P, et al. Locally injec-ted autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs. Vet Comp Orthop Trau-matol 2014; 27 (05): 379-86. doi: 10.3415/VCOT-14-02-0030.
Rainys D, Cepas A, Dambrauskaite K, Nedzelskiene I, Rimdeika R. Effecti-veness of autologous platelet-rich plasma gel in the treatment of hard-to-heal leg ulcers: a randomised control trial. J Wound Care 2019; 28(10): 658-67. doi: https://doi.org/10.12968/jowc.2019.28.10.658.
Yuan T, Zhang CQ, Tang MJ, Guo SC, Zeng BF. Autologous platelet rich plasma enhances healing of chronic wounds. Wounds 2009; 21: 280-5. doi: 10.1002/14651858.CD006899.pub3.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 R. Atasel Petin *, Çağrı Gültekin, Serkan Sayiner

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.