QUANTITATIVE ANALYSIS IN CANINE CUTANEOUS SOFT TISSUE SARCOMAS AND REACTIVE SPINDLE CELL PROLIFERATIONS ON CYTOLOGICAL SMEARS

Authors

  • Radostin S Simeonov Dept."General and Clinical Pathology of Animals" Faculty of Veterinary Medicine, Trakia University 6000 Stara Zagora, Bulgaria

Abstract

Stained cytological specimens from twenty-four canine cutaneous soft tissue sarcomas and twenty-four canine spindle cell reactive proliferations were analyzed by computer-assisted nuclear morphometry. In each case, the nuclei of at least 100 neoplastic and reactive spindle cells were measured. The studied morphometric parameter was nuclear roundness (NR). The aim of the present study was to evaluate the possibility of quantitative differentiation between canine cutaneous soft tissue sarcomas and reactive spindle cell proliferations. The mean values of NR were statistically significantly higher in soft tissue sarcomas than in non-neoplastic lesions. Statistical differences in NR were found between liposarcoma and haemangiopericytoma. Cytomorphometric differences between soft tissue sarcomas divided by histologic grade were not revealed. Differences, related to age, sex, breed or location of the lesions between non-neoplastic and neoplastic lesions were also not observed. The results indicated that the morphometric parameter NR could be used as an additional tool for differentiation between canine cutaneous soft tissue sarcomas and reactive spindle cell proliferations.

Key words: computer-assisted morphometry; nuclear roundness; soft tissue sarcomas; spindle cell proliferations; dog

 

KVANTITATIVNE ANALIZE KOŽNIH MEHKOTKIVNIH SARKOMOV PRI PSIH IN PROLIFERACIJE REAKTIVNIH VRETENASTIH CELIC V CITOLOŠKIH BRISIH

Namen predstavljene raziskave je bil kvantitativno ovrednotiti razliko med kožnimi mehkotkivnimi sarkomi in proliferacijo reaktivnih vretenastih celic pri psih. Z računalniško podprto analizo smo analizirali morfološke značilnosti jeder v 24 obarvanih prepratih kožnih mehkotkivnih sarkomov in 24 proliferacij reaktivnih vretenastih celic. Pri vsakem primeru smo analizirali vsaj 100 jeder in morfometrijsko določili okroglost jedra (OJ). Povprečna vrednost OJ je bila statistično značilno višja pri mehkotkivnih sarkomih kot pri neoplastičnih lezijah. Ugotovili smo tudi statistično značilne razlike v okroglosti jeder med liposarkomi in haemangiopericitomi. Med različnimi histološkimi tipi mehkotkivnih sarkomov, starostjo, spolom, pasmo in lokacijo poškodbe pa ni bilo citomorfometričnih razlik. Rezultati nakazujejo, da je morfometrični parameter OJ dodaten pokazatelj za razlikovanje med pasjimi mehkotkivnimi sarkomi in proliferacijami reaktivnih vretenastih celic.

Ključne besede: računalniško podprta morfometrija; okroglost jedra; mehkotkivni sarkomi; proliferacija vretenastih celic; pes

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Published

2017-04-02

How to Cite

Simeonov, R. S. (2017). QUANTITATIVE ANALYSIS IN CANINE CUTANEOUS SOFT TISSUE SARCOMAS AND REACTIVE SPINDLE CELL PROLIFERATIONS ON CYTOLOGICAL SMEARS. SLOVENIAN VETERINARY RESEARCH, 54(1). Retrieved from https://slovetres.si/index.php/SVR/article/view/208

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Original Research Article