EQUINE LEPTOSPIROSIS IN EGYPT: SEROPREVALENCE AND RISK FACTORS

Authors

  • Mohamed Marzok * Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Al-Hofuf, 31982, Saudi Arabia; Department of Surgery, Faculty of Veterinary Medicine, Kafr El Sheikh University, Kafr El Sheikh, Egypt, mmarzok@kfu.edu.sa
  • Abdelrahman M. Hereba Department of Biomedical Physics, Medical Research Institute, Alexandria University, Egypt; Department of Microbiology, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia
  • Abdelfattah Selim * Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt, abdelfattah.selim@fvtm.bu.edu.eg

DOI:

https://doi.org/10.26873/SVR-1716-2023

Keywords:

Leptospirosis, seroprevalence, risk factors, horse, Egypt

Abstract

Most leptospiral infections in horses are asymptomatic; however, acute disease manifestations as well as reproductive failure and recurrent uveitis have been reported. Horses are considered accidental hosts. The data about equine leptospirosis in Egypt are scarce. Hence, the present study aimed to investigate presence of antibodies against Leptospira sp. in horse in four Egyptian governorates and determine the associated risk factors for the infection. To determine the seroprevalence in 305 serum samples, the microscopic agglutination test (MAT) was carried using eight Leptospira servoars antigens. The results revealed 104 animals were positive for at least one of the serovars (34.1 %; 95 % CI: 29.01 - 39.59). The most common reaction was reported to Icterohaemorrhagiae servoar (15.14 %), followed by Canicola (14.75 %), Bratislava (11.47 %), Copenhageni (8.19 %), Pomona (7.86 %), and Hardjo (6.88 %). The most prevalent was observed among females, older, horses raising in pasture or in contact with ruminants or dogs and lack of rodent’s control. The significant seroprevalence suggests that Egyptian horses living in the studied area are at high risk of infection or exposure by Leptospira sp. Thus, the establishment of emergency surveillance and control program is very crucial for this zoonotic pathogen.

LEPTOSPIROZA KONJ V EGIPTU: SEROPREVALENCA IN DEJAVNIKI TVEGANJA

Izvleček: Večina leptospiroz pri konjih je asimptomatskih, vendar obstajajo poročanja o akutnih bolezenskih znakih, reprodukcijskih motnjah in ponavljajočih se uveitisih. Konji veljajo za naključne gostitelje. Podatki o leptospirozi konj v Egiptu so pomanjkljivi. Zato je bil namen te študije raziskati prisotnost protiteles proti bakteriji Leptospira sp. pri konjih v štirih egiptovskih pokrajinah in določiti z njimi povezane dejavnike tveganja za okužbo. Za določitev seroprevalence v 305 vzorcih seruma je bil opravljen mikroskopski aglutinacijski test (MAT) z osmimi antigeni serovara Leptospira. Rezultati so pokazali, da so bile 104 živali pozitivne na vsaj enega od serovarov (34,1 %; 95-odstotni indeks: 29,01–39,59). Najpogostejša reakcija je bila na serovar Icterohaemorrhagiae (15,14 %), sledili so Canicola (14,75  %), Bratislava (11,47 %), Copenhageni (8,19 %), Pomona (7,86 %) in Hardjo (6,88 %). Najpogostejše so bile pri kobilah, starejših konjih, konjih, ki se redijo na pašnikih ali so v stiku s prežvekovalci ali psi in pri pomanjkanju nadzora nad glodavci. Velika seroprevalenca kaže, da so egiptovski konji, ki živijo na proučevanem območju, izpostavljeni velikemu tveganju za okužbo z bakterijo Leptospira sp. Zato je za ta zoonotski patogen zelo pomembna vzpostavitev programa nujnega nadzora in obvladovanja. 

Ključne besede: leptospiroza; seroprevalenca; dejavniki tveganja; konj; Egipt

References

● Pappas G, Papadimitriou P, Siozopoulou V, Christou L, Akritidis N. The globalization of leptospirosis: worldwide incidence trends. Int J Infect Dis 2008; 12(4): 351–7.

● 2. Vijayachari P, Sugunan AP, Shriram AN. Leptospirosis: an emerging global public health problem. J Biosci 2008; 33(4): 557–69.

● 3. Bharti AR, Nally JE, Ricaldi JN, et al. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 2003; 3(12): 757–1.

● 4. Hamond C, Martins G, Lawson-Ferreira R, Medeiros MA, Lilenbaum W. The role of horses in the transmission of leptospirosis in an urban tropical area. Epidemiol Infect 2013; 141(1): 33–5.

● 5. Hamond C, Pinna A, Martins G, Lilenbaum W. The role of leptospirosis in reproductive disorders in horses. Trop Anim Health Prod 2014; 46(1): 1–10.

● 6. Whitwell K, Blunden A, Miller J, Errington J. Two cases of equine pregnancy loss associated with Leptospira infection in England. Vet Rec 2009; 165(13): 377–8.

● 7. Divers T, Chang YF, Irby N, Smith JL , Carter CN. Leptospirosis: an important infectious disease in North American horses. Equine Vet J 2019; 51(3): 287–92.

● 8. Verma A, Stevenson B, Adler B. Leptospirosis in horses. Vet Microbiol 2013; 167(1/2): 61–6.

● 9. Båverud V, Gunnarsson A, Engvall EO, Franzén P, Egenvall A. Leptospira seroprevalence and associations between seropositivity, clinical disease and host factors in horses. Acta Vet Scand 2009; 51(1): 1–10.

● 10. Pinto PS, Libonati H, Lilenbaum W. A systematic review of leptospirosis on dogs, pigs, and horses in Latin America. Trop Anim Health Prod 2017; 49(2): 231–8.

● 11. Daher EDF, Abreu KLS, Silva Junior GB. Insuficiência renal aguda associada à leptospirose. Braz J Nephrol 2010; 32(4): 408–15.

● 12. OIE. Leptospirosis. In: Manual of diagnostic tests and vaccines for terrestrial animals. 8th ed. Paris: OIE, 2018; 503–16.

● 13. Vemulapalli R, Langohr IM, Sanchez A, et al. Molecular detection of Leptospira kirschneri in tissues of a prematurely born foal. J Vet Diagn Invest 2005; 17(1): 67–71.

● 14. Selim AM, Elhaig MM, Gaede W. Development of multiplex real-time PCR assay for the detection of Brucella spp., Leptospira spp. and Campylobacter foetus. Vet Ital 2014; 50(4): 269–75.

● 15. Brandes K, Wollanke B, Niedermaier G, Brem S, Gerhards H. Recurrent uveitis in horses: vitreal examinations with ultrastructural detection of leptospires. J Vet Med A Physiol Pathol Clin Med 2007; 54(5): 270–5.

● 16. Hashimoto VY, Gonçalves DD, Silva FGd, et al. Occurrence of antibodies against Leptospira spp. in horses of the urban area of Londrina, Paraná, Brazil. Rev Inst Med Trop São Paulo 2007; 49(5): 327–30.

● 17. Samir A, Soliman R, El-Hariri M, Abdel-Moein K , Hatem ME. Leptospirosis in animals and human contacts in Egypt: broad range surveillance. Rev Soc Bras Med Trop 2015; 48(3): 272–7.

● 18. Ibrahim NA, Alrashdi BM, Elnaker YF, et al. Serological investigation and epidemiological analysis of bovine leptospirosis in Egypt. Trop Med Infect Dis 2022; 7(9): 208.

● 19. Martinez F. Leptospirosis in horses: a European perspective. Equine Vet J 2019; 51(3): 285–6.

● 20. Vera E, Taddei S, Cavirani S, et al. Leptospira Seroprevalence in bardigiano horses in northern Italy. Animals 2019; 10(1): e 23. doi: 10.3390/ani10010023

● 21. Houwers DJ, Goris MGA, Abdoel T, et al. Agglutinating antibodies against pathogenic Leptospira in healthy dogs and horses indicate common exposure and regular occurrence of subclinical infections. Vet Microbiol 2011; 148(2/4): 449–51.

● 22. Blatti S, Overesch G, Gerber V, Frey J, Hüssy D. Seroprevalence of Leptospira spp. in clinically healthy horses in Switzerland. Schweiz Arch Tierheilkd 2011; 153(10): 449–56.

● 23. Cerri D, Ebani V, Fratini F, Pinzauti P, Andreani E. Epidemiology of leptospirosis: observations on serological data obtained by a" diagnostic laboratory for leptospirosis" from 1995 to 2001. New Microbiol 2003; 26(4): 383–9.

● 24. Ebani VV, Bertelloni F, Pinzauti P, Cerri D. Seroprevalence of Leptospira spp. and Borrelia burgdorferi sensu lato in Italian horses. Ann Agric Environ Med 2012; 19(2): 237–40.

● 25. Hamond C, Martins G, Lilenbaum W. Subclinical leptospirosis may impair athletic performance in racing horses. Trop Anim Health Prod 2012; 44(8): 1927–30.

● 26. Dewes C, Fortes TP, Machado GB, et al. Prevalence and risk factors associated with equine leptospirosis in an endemic urban area in Southern Brazil. Braz J Dev 2020; 6(8): 58380–90.

● 27. Da Silva AS, Jaguezeski AM, Laber IF, et al. Leptospira spp. in horses in southern Brazil: seroprevalence, infection risk factors, and influence on reproduction. Comp Immunol Microbiol Infect Dis 2020; 73: e101552. doi:10.1016/j.cimid.2020.101552

● 28. Siqueira CC, Fraga DBM, Chagas-Junior AD, et al. Seroprevalence and risk factors associated with equine leptospirosis in the metropolitan region of Salvador and Recôncavo Baiano region, Bahia state (NE Brazil). Trop Anim Health Prod 2020; 52(1): 31–9.

● 29. Trimble AC, Blevins CA, Beard LA, Deforno AR, Davis EG. Seroprevalence, frequency of leptospiuria, and associated risk factors in horses in Kansas, Missouri, and Nebraska from 2016-2017. PLoS One 2018; 13(10): e0206639. doi:10.1371/journal.pone.0206639

● 30. Selim A, El-Haig M, Galila ES, Gaede W. Direct detection of Mycobacterium avium subsp. paratuberculosis in bovine milk by multiplex Real-time PCR. Anim Sci Pap Rep 2013; 31(4): 291–302.

● 31. Elhaig MM, Selim A, Mandour AS, Schulz C, Hoffmann B. Prevalence and molecular characterization of peste des petits ruminants virus from Ismailia and Suez, Northeastern Egypt, 2014–2016. Small Ruminant Res 2018; 169: 94–8.

● 32. Selim A, Ali A-F, Ramadan E. Prevalence and molecular epidemiology of Johne’s disease in Egyptian cattle. Acta Trop 2019; 195: 1–5.

● 33. Abdallah M-C, Kamel M, Karima B, et al. First report of Toxoplasma gondii infection and associated risk factors in the dromedary camel (Camelus dromedarius) population in south east Algeria. Vet Parasitol Reg Stud Rep 2020; 22: e100475. doi:10.1016/j.vprsr.2020.100475

● 34. Selim A, Abdelhady A. The first detection of anti-West Nile virus antibody in domestic ruminants in Egypt. Trop Anim Health Prod 2020; 52(6): 3147–51.

● 35. Raghavan R, Brenner K, Higgins J, Shawn Hutchinson JM , Harkin KR. Evaluations of hydrologic risk factors for canine leptospirosis: 94 cases (2002–2009). Prev Vet Med 2012; 107(1/2): 105–9.

● 36. Coiro CJ, Langoni H, da Silva RC. Epidemiological aspects in the Leptospira spp. and Toxoplasma gondii infection in horses from Botucatu, São Paulo, Brazil. J Equine Vet Sci 2012; 32(10): 620–3.

● 37. Rocha T, Ellis W, Montgomery J, Gilmore C, Regalla J, Brem S. Microbiological and serological study of leptospirosis in horses at slaughter: first isolations. Res Vet Sci 2004; 76(3): 199–202.

● 38. Wangdi C, Picard J, Tan R, Dowling B, Gummow B. Equine leptospirosis in tropical Northern Queensland. Aust Vet J 2013; 91(5): 190–7.

● 39. Langoni H, Da Silva A, Pezerico S, Lima VY.. Anti-leptospirose agglutinins in equine sera, from São Paulo, Goiás, and Mato Grosso do Sul, Brazil, 1996-2001. J Venom Anim Toxins Incl Trop Dis 2004; 10(3): 207–18.

● 40. Selim A, Abdelhady A. Neosporosis among Egyptian camels and its associated risk factors. Trop Anim Health Prod 2020; 52(6): 3381–5.

● 41. Selim A, Ali A-F. Seroprevalence and risk factors for C. burentii infection in camels in Egypt. Comp Immunol Microbiol Infect Dis 2020; 68: e101402.doi:10.1016/j.cimid.2019.101402

● 42. Selim A, Marawan MA, Ali A-F, Manaa E , AbouelGhaut HA. Seroprevalence of bovine leukemia virus in cattle, buffalo, and camel in Egypt. Trop Anim Health Prod 2020; 52(3): 1207–10.

● 43. Selim A, Megahed AA, Kandeel S, Abdelhady A. Risk factor analysis of bovine leukemia virus infection in dairy cattle in Egypt. Comp Immunol Microbiol Infect Dis 2020; 72: e101517. doi:10.1016/j.cimid.2020.101517

● 44. Selim A, Radwan A. Seroprevalence and molecular characterization of West Nile Virus in Egypt. Comp Immunol Microbiol Infect Dis 2020; 71: e101473. doi: 10.1016/j.cimid.2020.101473

● 45. Selim A, Radwan A, Arnaout F, Khater H. The recent update of the situation of West Nile fever among equids in Egypt after three decades of missing information. Pak Vet J 2020; 40(3): 390–3.

● 46. Marawan MA, Alouffi A, El Tokhy S, et al. Bovine Leukaemia Virus: current epidemiological circumstance and future prospective. Viruses 2021; 13(11): e2167. doi: 10.3390/v13112167

● 47. Selim A, Almohammed H, Abdelhady A, Alouffi A, Alshammari FA. Molecular detection and risk factors for Anaplasma platys infection in dogs from Egypt. Parasites Vector 2021; 14(1): e429. doi: 10.1186/s13071-021-04943-8

● 48. Martins G, Lilenbaum W. The panorama of animal leptospirosis in Rio de Janeiro, Brazil, regarding the seroepidemiology of the infection in tropical regions. BMC Vet Res 2013; 9: e237. doi:10.1186/1746-6148-9-237

● 49. Selim A, Manaa E, Khater H. Molecular characterization and phylogenetic analysis of lumpy skin disease in Egypt. Comp Immunol Microbiol Infect Dis 2021; 79: e101699. doi:10.1016/j.cimid.2021.101699

● 50. Selim A, Manaa E, Khater H. Seroprevalence and risk factors for lumpy skin disease in cattle in Northern Egypt. Trop Anim Health Prod 2021; 53(3): e350. doi:10.1007/s11250-021-02786-0

● 51. Selim A, Manaa EA, Alanazi AD, Alyousif MS. Seroprevalence, risk factors and molecular identification of bovine leukemia virus in Egyptian cattle. Animals 2021; 11(2): e319. doi:10.3390/ani11020319

● 52. Selim A, Megahed A, Kandeel S, Alouffi A, Almutairi MA. West Nile virus seroprevalence and associated risk factors among horses in Egypt. Sci Rep 2021; 11: e20932. doi:10.1038/s41598-021-00449-6

● 53. Chiareli D, Moreira E, Gutiérrez H, et al. Freqüência de aglutininas anti-Leptospira interrogans em eqüídeos, em Minas Gerais, 2003 a 2004. Arq Brasil Med Vet Zootec 2008; 60(6): 1576–9.

● 54. Barwick RS, Mohammed HO, McDonough PL, White ME. Epidemiologic features of equine Leptospira interrogans of human significance. Prev Vet Med 1998; 36(2): 153–65.

● 55. Tsegay K, Potts A, Aklilu N, Lötter C, Gummow B. Circulating serovars of Leptospira in cart horses of central and southern Ethiopia and associated risk factors. Prev Vet Med 2016; 125: 106–15.

● 56. Simbizi V, Saulez MN, Potts A, Lötter C, Gummow B. A study of leptospirosis in South African horses and associated risk factors. Prev Vet Med 2016; 134: 6–15.

● 57. Pinna MH, Varges R, Lilenbaum W. Aplicação de um programa integrado de controle da leptospirose em eqüinos no Rio de Janeiro, Brasil. Rev Brasil Ciência Vet 2008; 15(2): 63–6.

● 58. Batista CdSA, Alves CJ, Azevedo SSd, et al. Soroprevalência e fatores de risco para a leptospirose em cães de Campina Grande, Paraíba. Arq Brasil Med Vet Zootec 2005; 57(suppl. 2): 179–85.

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Published

2023-05-18

How to Cite

Marzok, M., Hereba, A. M., & Selim, A. (2023). EQUINE LEPTOSPIROSIS IN EGYPT: SEROPREVALENCE AND RISK FACTORS. Slovenian Veterinary Research, 60(2), 95–103. https://doi.org/10.26873/SVR-1716-2023

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Original Research Article

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