AMELIORATIVE EFFECTS OF VITAMIN E AND SELENIUM ON BLEOMYCIN-INDUCED MALE INFERTILITY
DOI:
https://doi.org/10.26873/SVR-1673-2023Keywords:
Bleomycin, selenium, vitamin E, vale fertilityAbstract
Bleomycin (BL) is a well-known anticarcinogenic chemotherapy that is used for the remedy of numerous varieties of most cancers. However, the use of BL became connected to the prevalence of several unfavorable results together with skin and lung fibrosis and testicular cancer. Dietary micronutrients such as vitamin E, and selenium (Se) are gambling critical roles in maintaining the health of human and animals and preserving the cell redox status homeostasis through their antioxidant roles. The objectives of the current investigation had been to investigate BL-induced male infertility in Albino rats. The protecting effects of either vitamin E, or Se towards such detrimental outcomes had been additionally examined. Moreover, the potential mechanisms behind such adverse effects had been evaluated. The obtained results indicated significant reduction in the testosterone, FSH, and LH levels in the BL-treated group. Likely, sperm characteristics as sperm count and motility had been significantly reduced in BL-treated group. Such changes have been associated with a significant reduction within the antioxidant enzymes including CAT, GPx, SOD, and MDA. Interestingly, co-exposure of experimental rats to either BL with vitamin E, or Se had notably parameters relatively close to the normal levels suggesting the ameliorative effects of vitamin E and Se towards BL-induced male infertility.
References
● 1. Dohle GR. Male infertility in cancer patients: review of the literature. Inter J Urol 2010; 17(4): 327–31.
● 2. Amirshahi T, Najafi G, Nejati V. Protective effect of royal jelly on fertility and biochemical parameters in bleomycin- induced male rats. Iran J Reprod Med 2014; 12(3): 209.
● 3. Khadivi F, Razavi S, Hashemi F. Protective effects of zinc on rat sperm chromatin integrity involvement: DNA methylation, DNA fragmentation, ubiquitination and protamination after bleomycin etoposide and cis-platin treatment. Theriogenol 2020; 142: 177–83.
● 4. Liu M, Hales BF, Robaire B. Effects of four chemotherapeutic agents, bleomycin, etoposide, cisplatin, and cyclophosphamide, on DNA damage and telomeres in a mouse spermatogonial cell line. Biol Reprod 2014; 90(4): 72–1.
● 5. Maselli J, Hales BF, Robaire B. The effects of chemotherapy with bleomycin, etoposide, and cis-platinum (BEP) on rat sperm chromatin remodeling, fecundity and testicular gene expression in the progeny. Biol Reprod 2013; 89(4): 85–1.
● 6. Gokce A, Oktar S, Koc A, et al. Protective effects of thymoquinone against methotrexate‐induced testicular injury. Human Exper Toxicol 2011: 30: 897–903.
● 7. Bolle P, Evandri MG, Saso L. The controversial efficacy of vitamin E for human male infertility. Contraception 2002: 65(4): 313-–5.
● 8. Hashemi F, Razavi S, Khadivi F. The protective effects of omega3 on ubiquitination and protamination of rat sperm after bleomycin, etoposide, and cisplatin treatment. Nutr Cancer 2018; 70(8): 1308–14.
● 9. Moslemi MK, Tavanbakhsh S. Selenium–vitamin E supplementation in infertile men: effects on semen parameters and pregnancy rate. Inter J Gen Med 2011; 4: 99.
● 10. Ahsan U, Kamran Z, Raza I, et al. Role of selenium in male reproduction—A review. Anim Reprod Sci 2014; 146(1-2): 55–62.
● 11. Wen JC. The role of vitamin E in the treatment of male infertility. Nutr Bytes 2006; 11: 1.
● 12. Igweze ZN, Orisakwe OE, Obianime AW. Reproductive parameter in 90 day toxicity study of smart Herbal purifierR-a poly Herbal supplement in male rats. J Appl Pharm Cetri Sci 2014; 4(10): 069–74.
● 13. Burtis CA, Ashwood FR. Textbook of clinical chemistry, 2nd ED, WB Saunders Co., Philadephia, 1994; p1851.
● 14. Slott V, Suarez J, Perreault S. Rat sperm motility analysis: methodologic considerations. Reprod Toxicol 1991; 5: 449–58.
● 15. Filler R. Methods for evaluation of rat epidydimal sperm morphology. In: Methods in toxicology, vol.3 (part A). Male reproductive toxicology. Chapin RE, Heindel JJ (eds). Academic Press Limited, London, UK, 1993; 334–43.
● 16. Andersen RA, Ingebrigtsen K, Nafstad I, et al. Biochemical characteristics of rat superoxide dismutase and the effect caused by paraquat injection on the enzyme activity in various tissues. Gen Pharmacol 1984; 15(3): 205–10.
● 17. Kilarkaje N, Mousa AM, Al-Bader MM, et al. Antioxidants enhance the recovery of three cycles of bleomycin, etoposide, and cisplatin–induced testicular dysfunction, pituitary-testicular axis, and fertility in rats. Fertil Steril 2013; 100(4): 1151–9.
● 18. Yaghutian Nezhad L, Mohseni Kouchesfahani H, Alaee S, et al. Thymoquinone ameliorates bleomycin-induced reproductive toxicity in male Balb/c mice. Human Exper Toxicol 2021; 40: S611–21.
● 19. Kessopoulou E, Powers HJ, Sharma KK, et al. A double-blind randomized placebo cross-over controlled trial using the antioxidant vitamin E to treat reactive oxygen species associated male infertility. Fertil Steril 1995; 64(4): 825–31.
● 20. Keskes-Ammar L, Feki-Chakroun N, Rebai T, et al. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl 2003; 49(2): 83–94.
● 21. Safarinejad MR, Safarinejad S. Efficacy of selenium and/or N-acetyl-cysteine for improving semen parameters in infertile men: a double-blind, placebo controlled, randomized study. J Urol 2009; 181(2): 741–51.
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Copyright (c) 2023 Hosny Abd El-Fadil Ibrahim, Shimaa I. Shalaby, Ahmed Abdelfattah-Hassan, Rasha Mahmoud M. A. Hebishy, Eman Mohamed Abdel Mohsen Abdel Ghani
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