PHENOTYPIC CHARACTERIZATION OF SOME PATHOGENIC FUNGI ISOLATED FROM POULTRY AND THEIR SURROUNDINGS IN EL–GHARBIA GOVERNORATE, EGYPT
DOI:
https://doi.org/10.26873/SVR-1632-2022Keywords:
phenotypic, fungi, poultry, antifungal sensitivityAbstract
Poultry production is affected by several fungal diseases. Such fungal infection occurs in poultry farms via using a moldy litter, or ingestion of contaminated drinking water or moldy ration. In this study, a total of 210 birds with a history of respiratory distress of different breeds were collected randomly from sporadic different private farms and hatcheries in El–Gharbia Governorate, Egypt. The birds were sacrificed, then a total of 1050 tissue specimens from lung, air sacs, liver, crop and trachea were collected. In addition, 40 samples of poultry ration, 14 bedding materials, 4 air samples and 29 water samples were also collected. The collected samples were cultured on Sabouraud’s agar plates. Macromorphological and micromorphological fungal examinations were performed for phenotypic characterization. Histopathological examinations were also performed using with hematoxylin and eosin stains. Antifungal sensitivity testing was screened using Mueller’s Hinton Agar for studying the susceptibility of the recovered fungal isolates to the most commonly used antifungal drugs in Egypt, namely amphotericin B, clotrimazole, fluconazole, itraconazole, ketoconazole, and nystatin. The obtained results demonstrated that mold isolation was the highest in the collected samples from birds at 36.84%, followed by drinking water (31.57%). The highest incidence of mold isolation was recorded at the lungs of broilers and baladi birds followed by the air sacs. While in saso birds, the highest incidence was at the air sac. Collectively, 97 mold strains were identified from the lung, 74 from the air sacs, 30 from the liver, 61 from the trachea, and 44 from the crop. In addition, 19 mold isolates were recovered from the bird surroundings. Aspergillus niger as well as Penicillium chrysogenum were recovered and showed resistance to ketoconazole, while Cladosporium perangustum was resistant to fluconazole. All of the isolated molds were sensitive to itraconazole and nystatin except A. flavus that was resistant to nystatin. All Aspergillus spp. were resistant to fluconazole except A. niger. In conclusion, Aspergillus spp. was the most associated mold with poultry species and their surroundings in Egypt farms. Itraconazole and nystatin could be applied as proper antifungal drugs the control of for Aspergillus infection in birds.
References
● 1. Fagbohun ED, Ayantola KJ, Toyin-Famoroti A J. Isolation and molecular characterization of Aspergillus fu-migatus and Aspergillus flavus isolated from poultry birds in Ado-Ekiti, Nigeria. Asian J Biotechnol Bioresour Technol, 2020; 6(2), 31–44.
● 2. Radwan, IA, Abed AH, Abd El-Aziz MM. Fungal pathogens associated with respiratory problems in broiler chickens. J Vet Med Res, 2016; 23(1), 1–8.
● 3. Asfaw M Dawit D. Review on major fungal disease of poultry. Br J Poult Sci, 2017; ( 6), 16-25.
● 4. Mohamed S, Awad A, Elsaedy Y, Younis G. De-tection of mold species in poultry farms in refer to their virulence potential. Mansoura Vet Med J, 2020; 21(1), 6–13.
● 5. Mohammed AN, Abdel-Latef GK. Environmental Monitoring of Zoonotic Fungal Infection in Broiler Chick-ens: Novel Approach to Control using Nano-fungicide Composite. Tropical Animal Science Journal, 2021; 44(3), 336–46.
● 6. Girma G, Abebaw M, Zemene M, Mamuye Y, & Getaneh G. A review on aspergillosis in poultry. J. Vet. Sci. Technol, 2016; 7(6).
● 7. Thathana MG, Murage H, Abia ALK, & Pillay M. Morphological characterization and determination of afla-toxin-production potentials of Aspergillus flavus isolated from maize and soil in Kenya. Agriculture, 2017; 7(10), 80.
● 8. Bancroft KSS, Christopher L, John D. Bancroft. The Hematoxylins and Eosin. In: SUVARNA, K. S. (ed. ) Bancroft's Theory and Practice of Histological Techniques. 8th ed. Sheffield, UK: Elsevier, 2013; 4526–30.
● 9. APHA(American Public Health Association. Com-pendium of methods for the microbiological examination of food, 3rd Ed. American public Health Association, Washington, D. C. , 1992.
● 10. Osaro-Methew, Ruth-Chiamaka & otiekwa chidinmia. Microbial Analysis of Poultry Feeds Produced in Songhai Farms, Rivers State, Nigeria. Journal of Microbiol-ogy & Experimentation. Department of microbiology, Michael Okpara University of Agriculture Umudike, Nigeria, 2017; (4).
● 11. Barnett HL, Hunter BB. Illustrated Genera of Im-perfect Fungi. Third Edition. Burgess Publishing Company, Minneapolis. 1972; 241 pp.
● 12. Napoli C, Marcotrigiano V, and Montagna MT. Air sampling procedures to evaluate microbial contamina-tion: a comparison between active and passive methods in operating theatres, BMC Public Health 2012; 12:594, http://www. biomedcentral. com/1471-2458/12/594
● 13. Kinsey GC, Paterson RR & Kelley J. Methods for the determinationof filamentous fungi in treated and un treated waters, journal of Applied MicrobiologySypplement, 1999; (85) 214S-224S.
● 14. Collins and Lyne. Microbiological method, 5th Ed. Microbiology laboratory manuals. British Liberary Butter Worth in co. , 1984.
● 15. Arné P, Thierry S, Wang D, Deville M, Loc'h L, Desoutter A& Guillot J. Aspergillus fumigatus in poultry. International journal of microbiology, 2011; 74–6.
● 16. Viegas C, Viegas S, Sabino R, Verissimo C. Venti-lation influence in occupational exposure to fungi and vola-tile organic compounds:poultry case; 2011.
● 17. Abd El Tawab AA, Maarouf AA, El-Hofy FI & Ahmed K. Molecular characterization of some fungi isolat-ed from broiler chicken farms. Benha Veterinary Medical Journal, 2015; 29(2), 106–18.
● 18. Ostović M, Ravić I, Kovačić M, Kabalin AE, Matković K, Sabolek I, et al. . Differences in fungal contam-ination of broiler litter between summer and winter fatten-ing periods. Arh Hig Rada Toksikol, 2021; (72) 140–7.
● 19. Radwan I, Abed A& Abdallah A. Antifungal ef-fect of carvacrol on fungal pathogens isolated from broiler chickens. Assiut Vet Med J, 2018; ( 64)11–7.
● 20. Abed A, Radwan I, El-Aziz M& Ali A. Antifun-gal activity of natural essential oils against molds and yeasts associated with respiratory problems in broiler chickens. Adv Anim Vet Sci, 2021; ( 9)348–55.
● 21. Byrd J, Caldwell D& Nisbet D. The identification of fungi collected from the ceca of commercial poultry. Poult Sci J, 2017; ( 96) 2360–5.
● 22. Cheng Z, Li M, Wang Y, Chai T, Cai Y& Li N. Pathogenicity and Immune Responses of Aspergillus fumiga-tus Infection in Chickens. Front Vet Sci, 2020; ( 7) 143–50
● 23. Abd El-Aziz RM. Characterization and differenti-ation of fungal and bacterial isolates associated with dead-in-shell embryos in breeder layer. MV SC (Doctoral disserta-tion, Thesis, Microbiology Dept. , Beni-Suef Univ. , Beni-Suef, Egypt; 2015.
● 24. Viegas C, Carolino E, Malta-Vacas J, Sabino R, Viegas S& Veríssimo C. Fungal contamination of poultry litter: a public health problem. Journal of Toxicology and Environmental Health, Part A, 2012; 75(22-23), 1341–50.
● 25. Viegas C, Viegas S, Monteiro A, Carolino E, Sabino R & Verissimo C. . Air contaminants in animal production: the poultry case. Air Pollution, 2012b ; (157) 315–26. .
● 26. Kannoju A, Veldi P& Kumar V. An overview of aspergillosis in poultry: A review. J Entomol Zool, 2021; (9) 685–8.
● 27. Dhama K, Chakraborty S, Wani MY, Verma AK, Deb R, Tiwari R, & Kapoor S. Novel and emerging thera-pies safeguarding health of humans and their companion animals: a review. Pakistan Journal of Biological Sciences: PJBS, 2013; 16(3), 101–11.
● 28. Kaur J, Brar RS, Banga HS & Chandra M. Diagno-sis of aspergillosis in avian species from Punjab, The Phar-ma Innovation Journal, 2019; 8(7): 418–20.
● 29. Nururrozi A, Yanuartono Y, Widyarini S, Ra-mandani D, Indarjulianto S. Clinical and pathological fea-tures of aspergillosis due to Aspergillus fumigatus in broilers, Veterinary World, 2020; 13(12): 2787–92.
● 30. Abdul Hamid, FF, Reduan, MFH, Jasni, J, Chung, ELT&Nordin, MLb, Jesse, FFA, Rajdi NZIM &Kamaruzaman INAB & Shaharulnizim N. Aspergillosis concurrent with secondary bacterial infection in broiler chicks: a case report, Comparative Clinical Pathology, 2021; (30):341–5.
● 31. Ibrahim MAEH, Salem R, Diasty E & El-Mesalamy M. Molecular characterization of pathogenic mould and yeast isolated from poultry farms with detection of mycotoxins residues. Arab Journal of Nuclear Sciences and Applications, 2019; 52(4), 208–17.
● 32. Krnjaja V, Stojanović L, Cmiljanić R, Trenkovski S, & Tomašević D. The presence of potentially toxigenic fungi in poultry feed. Biotechnology in Animal Husbandry, 2008; 24(5-6), 87–93.
● 33. Mgbeahuruike A, Aliyoo K, Karlsson M& Chah K. Identification, Antifungal Susceptibility and Phylogenet-ic Comparison of Fungi in Poultry Environment in Nigeria. Int J Poult Sci, 2020; 43(8).
● 34. Denning DW, Cadranel J, Beigelman-Aubry C, Ader F, Chakrabarti A, Blot S, et al. . Chronic pulmonary aspergillosis: rationale and clinical guidelines for diagnosis and management. Eur Respir J, 2016; (47) 45–68.
● 35. Verweij PE, Ananda-Rajah M, Andes D, Ar-endrup MC, Brüggemann RJ, Chowdhary A, et al. . Interna-tional expert opinion on the management of infection caused by azole-resistant Aspergillus fumigatus. Drug Resist Updat, 2015; 21(22)30–40.
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