ANTIMICROBIAL RESISTANCE PROFILING OF SELECTED E. coli ISOLATES AND DETECTION OF ESBL/PAMPC-ENCODING GENES IN BROILER FLOCKS IN BOSNIA AND HERZEGOVINA USING REAL-TIME PCR

Authors

  • Amira Koro-Spahić Department of Animal Production and Biotechnology and Department of Pathobiology and Epidemiology, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
  • Sead Hadžiabdić Unit Food Hygiene and Technologies, Supply Chains, Food Defense, Department of Biological Safety, Max-Dohrn-Str.8-10, 10589 Berlin, Germany
  • Teufik Goletić Department of Animal Production and Biotechnology and Department of Pathobiology and Epidemiology, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
  • Amer Alić Department of Clinical Sciences of Veterinary Medicine, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
  • Aida Kustura Department of Animal Production and Biotechnology, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
  • Adis Softić Department of Animal Production and Biotechnology, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
  • Emina Residbegović * Department of Animal Production and Biotechnology and Department of Pathobiology and Epidemiology, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina

DOI:

https://doi.org/10.26873/SVR-1630-2025

Keywords:

antimicrobial resistance, E. coli, broilers, Bosnia and Herzegovina

Abstract

Antimicrobial resistance (AMR) is a growing global issue, driven by the nontargeted use of antimicrobials in livestock. Poultry, particularly broilers, may serve as significant reservoirs for resistant Escherichia (E.) coli strains. This study aimed to isolate E. coli from broiler flocks and evaluate their in vitro susceptibility towards β-lactams, cephalosporins, carbapenems, tetracyclines, and fluoroquinolones. Additionally, a multiplex real-time PCR assay was used to detect extended-spectrum β-lactamase (ESBL)- and carbapenemase-encoding genes. A total of 48 commensal E. coli isolates from broiler flocks in Bosnia and Herzegovina (BiH) were analyzed. Phenotypic resistance, determined using the disc diffusion method, was observed for ampicillin (87.5%), amoxicillin/clavulanic acid (62.5%), cefepime (41.7%), cefoxitin (45.8%), cefotaxime (50.0%), ceftazidime (47.9%), azithromycin (58.3%), ciprofloxacin (66.7%), and tetracycline (72.9%). PCR analysis confirmed blaTEM, blaCTX-M and blaCMY genes in 24 isolates (50%), whereas blaSHV and carbapenemase-encoding genes (blaKPC, blaNDM, blaOXA-48, blaVIM and blaGES) were not detected. The high prevalence of multidrug-resistant E. coli strains highlights the need for enhanced antimicrobial stewardship in poultry production. Reducing antibiotic use, promoting alternative disease control measures, and implementing systematic resistance monitoring programs are crucial to reduce AMR in broiler farms and potential spill over to public health.

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2025-07-18

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Koro-Spahić, A., Hadžiabdić, S., Goletić, T., Alić, A., Kustura, A., Softić, A., & Residbegović, E. (2025). ANTIMICROBIAL RESISTANCE PROFILING OF SELECTED E. coli ISOLATES AND DETECTION OF ESBL/PAMPC-ENCODING GENES IN BROILER FLOCKS IN BOSNIA AND HERZEGOVINA USING REAL-TIME PCR. Slovenian Veterinary Research, Early View. https://doi.org/10.26873/SVR-1630-2025

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Copyright (c) 2025 Amira Koro-Spahić, Sead Hadžiabdić, Teufik Goletić, Amer Alić, Aida Kustura, Adis Softić, Emina Residbegović *

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