• Hadeil Alsufiani Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
  • Ghaynat A. Aldhaheri Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
  • Ulfat M. Omar Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Princess Dr. Najla Bint Saud Al-Saud Center for Excellence Research in Biotechnology, King Abdulaziz University, Jeddah, Saudi Arabia
  • Taraji M. Bahdilah Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
  • Rasha A. Mansouri Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia



2,4,4'-trihydroxychalcone, DPPH radical scavenging activity, reducing power, ferrous ion chelating activity, sucrase, α-amylase, lipase


The aim of this study was to investigate the antioxidant activity and inhibitory effect of 2,4,4'-trihydroxychalcone on digestive enzymes related to obesity, including sucrase, α-amylase, and lipase. The in vitro antioxidant activities of three concentrations of 2,4,4'-trihydroxychalcone (100, 300, and 500 µg/ml) were determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging, reducing power, and ferrous ion chelating assays. Moreover, in vitro inhibition of lipase, α-amylase, and sucrase enzyme activities by 2,4,4'-trihydroxychalcone was determined using a specific assay for each enzyme. 2,4,4'-Trihydroxychalcone has been shown to have antioxidant properties and inhibits sucrase, α-amylase and lipase activities. These findings suggest that 2,4,4′-trihydroxychalcone demonstrated an antioxidant activity and can effectively inhibit the key enzymes related to obesity.


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Veterinary Medicine and The One Health Concept