PREVALENCE, ANTIMICROBIAL RESISTANCE PROFILES OF CLINICAL AND SUBCLINICAL MASTITIS IN LACTATING COW WITH ASSESSMENT OF TREATMENT TRIAL

Authors

  • Wafaa El-Neshwy Department of Animal Medicine, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt, *Corresponding author, E-mail: wafaa892011@gmail.com
  • Abo-Zaid, A.A., Department of Animal Medicine, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt
  • El-Mekawey, M.F Department of Animal Medicine, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt
  • Soliman, A.H Department of Animal Medicine, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt

DOI:

https://doi.org/10.26873/SVR-1618-2022

Keywords:

bovine mastitis, drug resistance, resistance gens, treatment, Cafalexin, Kanamycin

Abstract

This work investigated the prevalence of bovine mastitis in a dairy farm, Ismalia governrate, Egypt with phynotypic and genotypic clarification of the causative bacteria and their antimicrobial susceptibility. Also a treatment trial with a combination of Cafalexin, Kanamycin was evaluated. The total prevalence of mastitis was 31.82% (119/374) at cow level and 17.01% (247/1452) at quarter level. 261 isolates were detected. (74.33%) belong to staphylococcus spp and (25.67%) to streptococcus spp. as major microorganisms (CNS (42.53%) s.aurus (31.80%) s. uberis (12.26%), s. agalactia (8.81%) and s. dysagalactia(4.59%). The isolates were tested against 15 antimicrobial agents and the highest percentage of resistant bacteria was for AMC, P, C and E. while the lowest rate was  for CN, CIP, CL and K. Molecular Characterization of isolated pathogens and antimicrobial resistance genes was performed by PCR on 15 isolates. blaTEM-1 Was the most frequently detected gen followed by aadA1, dfrA1, cmlA, sul1, and tetA. 110 infected udder quarters were enrolled for 21 days to evaluate the treatment with Terrexine LC intramammary suspension 10g on six occasions at 12 h intervals and gentamycin intramuscular injection (1cm/20kg Bw for 3-5 days in cows with systemic reaction. A high significant reduction was recorded for the log10 SSC, log10 TBC and the level of LDH in milk after treatment  compared their level before treatment (P-value <0.0001***).  The milk season for cows, degree of mastitis or type of microorganism isolated before treatment have no effect on the recovery rate P-value ˃0.05.  In conclusion, the emergence of multidrug-resistant strains is greatly increased so antibiotic usage must be Restricted.  PCR can help in the rapid detection of the resistant strains.  Treatment of mastitis with combination of antimicrobial may reduce drug resistance. Bovine mastitis still needs updated knowledge on the causative microbes and their antibiotic resistance patterns for optimal control and treatment.

References

● 1. Miller G, Bartlett P, Lance S, Anderson J, Heider L. Costs of clinical mastitis and mastitis prevention in dairy herds. J Am Vet Med Assoc 1993; 202: 1230–36.

● 2. Gomes F, Henriques M. Control of bovine mastitis: old and recent therapeutic approaches. Curr. Microbiol 2016; 72: 377–82.

● 3. Ruegg P. What is success? A narrative re- view of research evaluating outcomes of antibiot- ics used for treatment of clinical mastitis. Front Vet Sci 2021; 8: 639-41.

● 4. Zadoks N, Gillespie B, Barkema W, Sampi- mon C, Oliver P, Schukken H. Clinical, epidemi- ological and molecular characteristics of Strepto- coccus uberis infections in dairy herds. Epidemiol Infect 2003; 130: 335–49.

● 5. Perreten V, Endimiani A, Thomann A, et al. Evaluation of PCR electrospray-ionization mass spectrometry for rapid molecular diagnosis of bo- vine mastitis. J Dairy Sci 2013; 96(6):3611–20.

● 6. Chagunda M, Larsen T, Bjerring M, Ing- vartsen L. L-lactate dehydrogenase and N-ace- tyl-β-D-glucosaminidase activities in bovine milk as indicators of non-specific mastitis. J Dairy Res 2006; 73: 431–40.

● 7. Nyman K, Waller P, Bennedsgaard T, Larsen T, Emanuelson U. Associations of udder-health indicators with cow factors and with intramam- mary infection in dairy cows. J Dairy Sci 2014; 97: 5459–73.

● 8. Souza F, Cunha A, Rosa D, et al. Somat- ic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira 2016; 36(9): 811–18.

● 9. Pradhan P, Gopinath S, Reddy G, Decham- ma H, Suryanarayana, V. Detection of major pathogens in bovine sub-clinical mastitis by mul- tiplex pcr directly from milk samples in presence of an internal control. Indian J Fund Appl Life Sci 2011; 1 (4): 209–18.

● 10. Hillerton J, Berry E. A review. Treating mastitis in the cow-a tradition or an archaism. J App microbiol 2005; 98: 1250–55.

● 11. Pillai K, Moellering R, Eliopoulos M. Antimicrobial combinations.Antibiot Lab Med 2005; 5: 365–440.

● 12. Davis B. Bactericidal synergism between β-lactams and aminoglycosides: mechanism and possible therapeutic implications. Rev Infect Dis 1982;4: 237–45.

● 13. Ganière J, Denuault L. Synergistic interac- tions between cefalexin and kanamycin in Muel- ler–Hinton broth medium and in milk. J App Mi- crobiol 2009; 107: 117–25.

● 14. Clements A, Taylor D, Fitzpatrick J. Eval- uation of diagnostic procedures for subclinical mastitis in meat-producing sheep. J Dairy Res 2003; 70: 139–48.

● 15. Barrow G, Feltham R. Cowan and Steel’s Manual for the Identification of Medical Bacteria 3rd ed. Cambridge: University Press, 1993.

● 16. Finegold S, Martin, W. “Diagnostic Micro- biology,” 6th Edition. C.V. Mosby Co. St. Louis, Toronto, London 1982: 199–239.

● 17. (CLSI) Clinical and Laboratory Standards Institute. Performance Standards for Antimicro- bial Susceptibility Testing; Twenty-Fifth Informa- tional Supplement, 2015; 35 (3): 1–240.

● 18. Falagas M, Koletsi P, Bliziotis I. The diver- sity of definitions of multidrug-resistant (MDR) and pandrug-resistant (PDR) Acinetobacter bau- mannii and Pseudomonas aeruginosa. J Med Mi- crobiol 2006; 55:1619–29.

● 19. Papich MG. Antimicrobials, susceptibility testing, and minimum inhibitory concentrations (MIC) in veterinary infection treatment. Veterinary Clinics: Small Anim Practice 2013; 43: 1079–89.

● 20. Zhang K, Sparling J, Chow L et al. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous dis- crimination of Staphylococcus aureus from coagulase-negative staphylococci. J Clin Microbiol 2004; 42: 4947–55.

● 21. Parbhu K, Isloor S, Hedge R, Rathnamma D, Veeregowda B, Suryanarayana V. Development of Polymerase Chain Reaction for detection of pre- dominant streptococcal isolates causing subclin- ical bovine mastitis. Indian. J Biotechnol 2013; 12: 208–12.

● 22. Yang H, Lee Y, Pan Y, Su W, Chuang Y. Prevalence and molecular characterization of plasmidmediated beta-lactamase genes among nosocomial Staphylococcus sppaureus isolated in Taiwan. TROP J PHARM RES 2017; 16: 155–60.

● 23. Randall L, Cooles S, Osborn M, Piddock L,Woodward M, Antibiotic resistance genes, inte- grons and multiple antibiotic resistance in thir- ty-five serotypes of Salmonella enterica isolated from humans and animals in the UK, J Antimicrob Chemother 2004; 53 (2): 208–16.

● 24. Grape M, Motakefi A, Pavuluri S, Kahl- meter G. Standard and real-time multiplex PCR methods for detection of trimethoprim resistance dfr genes in large collections of bacteria. Clin Mi- crobiol Infect 2007; 13(11): 1112–18.

● 25. Rather M, Aulakh R, Gill J, Mir A, Hassan M. Detection and sequencing of plasmid encod- ed tetracycline resistance determinants (tetA and tetB) from food–borne Bacillus cereus isolates. Asian Pac J Trop Med 2012; 5(9):709–12.

● 26. Enne V, Livermore D, Stephens P, Hall L. Persistence of sulphonamide resistance in Escherichia coli in the UK despite national pre- scribing restriction. Lancet. England 2001;357(9265):1325–8.

● 27. Van T, Chin J, Chapman T, Tran L, Coloe P. Safety of raw meat and shellfish in Vietnam: an analysis of Escherichia coli isolations for antibi- otic resistance and virulence genes. Inter J Food Microbiol 2008; 124: 217–223.

● 28. Team R. A language and environment for statistical computing. R Foundation for Statistical Computing.Vienna, Austria 2013.

● 29. Seegers H, Fourichon C, Beaudeau F. Pro- duction effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary re- search 2003; 34: 475–91.

● 30. Elbably M, Emeash H, Asmaa N. Risk fac- tors associated with mastitis occurrence in dairy herds in Benisuef, Egypt. World’s Vet J 2013; 3: 5–10.

● 31. Algammal A, Enany M, El-Tarabili R, Ghobashy M, Helmy Y. Prevalence, antimicrobial resistance profiles, virulence and enterotoxins-de- terminant genes of MRSA isolated from subclinical bovine mastitis in Egypt. Pathogens 2020; 9: 362.

● 32. Mbindyo C, Gitao G, Plummer P, Kuloho- ma B, Mulei C, Bett R. Antimicrobial resistance profiles and genes of Staphylococci isolated from mastitic cow’s milk in Kenya. Antibiotics 2021; 10: 772.

● 33. Oliveira C, Hogeveen H, Botelho A, Maia P, Coelho S, Haddad J. Cow-specific risk factors for clinical mastitis in Brazilian dairy cattle. Prev Vet Med 2015; 121: 297–305.

● 34. Condas L, De Buck J, Nobrega D, Carson D, Roy J, Keefe G, et al. Distribution of non-au- reus staphylococci species in udder quarters with low and high somatic cell count, and clinical mas- titis. J Dairy Sci 2017; 100: 5613–27.

● 35. León-Galván M, Barboza-Corona J, Lechuga-Arana A, Valencia-Posadas M, Aguayo D, Cedillo-Pelaez C, et al. Molecular detection and sensitivity to antibiotics and bacteriocins of pathogens isolated from bovine mastitis in fami- ly dairy herds of central Mexico. Biomed Res Int 2015; 615153.

● 36. Vakkamäki J, Taponen S, Heikkilä A, Pyörälä S. Bacteriological etiology and treatment of mastitis in Finnish dairy herds. Acta Vet Scand 2017; 59: 1–9.

● 37. Taponen S, Simojoki H, Haveri M, Larsen H, Pyörälä S. Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP. Vet microbiol 2006;115:199–207.

● 38. Timms L, Schultz L. Dynamics and signifi- cance of coagulase-negative staphylococcal intra- mammary infections. J Dairy Sci 1987;70:2648–57.

● 39. De Vliegher S, Barkema H, Stryhn H, Op- somer G, de Kruif A. Impact of early lactation so- matic cell count in heifers on milk yield over the first lactation. J Dairy Sci 2005; 88: 938–47.

● 40. Mousa W, Zaghawa A, Nayel M. Studies on clinical and subclinical mastitis in Menoufia Governate with application of PCR for diagnosis. JCVR 2015; 9: 78–84.

● 41. Mostafa A, Hammad A. Phenotypic and Molecular Characterization of Methicillin-Resis- tant Staphylococcus Aureus Isolated from Bovine Mastitis in Egypt. JCVR 2021;3:23–29.

● 42. El-Ashker M, Gwida M, Tomaso H, Monecke S, Ehricht R, El-Gohary F, et al. Staphylococci in cattle and buffaloes with mastitis in Dakahlia Gov- ernorate, Egypt. J Dairy Sci 2015; 98: 7450–59.

● 43. Abdel-Tawab A, El-Hofy F, Maarouf A, Abbas S. Molecular detection of some virulence genes of S. aureus isolated from mastitic Cows by PCR. Benha Veterinary Medical Journal (BVMJ). 2016; 30: 238–45.

● 44. El Faramaway R, Abdeen E, Ashraf A, Mou- sa W. Antibiogram Profile and Molecular Char- acterization of coa and spa Genes Of Methicillin Resistant Staphylococcus sppaureus (MRSA) from Clinical Mastitis. Alex J Vet Sci. 2019; 61 (1): 32–8.

● 45. Scherrer D, Corti S, Muehlherr J, Zweifel C, Stephan R. Phenotypic and genotypic charac- teristics of Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep. Vet Microbiol 2004; 101:101–07.

● 46. Duse A, Persson-Waller K, Pedersen K. Microbial aetiology, antibiotic susceptibility and pathogen-specific risk factors for udder patho- gens from clinical mastitis in dairy cows. Animals 2021; 11: 2113.

● 47. Hasan M, Islam M, Runa N, Uddin A, Singh S. Study on bovine sub-clinical mastitis on farm condition with special emphasis on antibiogram of the causative bacteria. Bangladesh. J Vet Med 2016; 14:161–6.

● 48. Abd El-Aziz NK, Ammar AM, El Damaty HM, Abd Elkader RA, Saad HA, El-Kazzaz W, et al. Environmental Streptococcus spp uberis associat- ed with clinical mastitis in dairy cows: virulence traits, antimicrobial and biocide resistance, and epidemiological typing. Animals 2021;11:1849.

● 49. Yang W, Ke C, Wu, Lee R, Tseng Y. Effective treatment of bovine mastitis with intramammary infusion of Angelica dahurica and Rheum offici- nale extracts. Evid. Based Complementary Altern. Med 2019;2019: ID 7242705.

● 50. Yang C, Lee S, Pan H, Su P, Chuang L. Prevalence and molecular characterization of plasmidmediated beta-lactamase genes among nosocomial Staphylococcus aureus isolated in Taiwan. Trop J Pharm Res 2017;16:155–60.

● 51. Pinzón-Sánchez C, Ruegg P. Risk factors associated with short-term post-treatment out- comes of clinical mastitis. J Dairy Sci 2011; 94 (7): 3397–410.

● 52. McDougall S. Intramammary treatment of clinical mastitis of dairy cows with a combi- nation of lincomycin and neomycin, or penicil- lin and dihydrostreptomycin. N Z Vet J 2003; 51: 111–16.

● 53. Krabbenhoft K, Adams A, Schipper I. Antibiotic sensitivities of organisms isolated from mastitic and nonmastitic mammary secretions. Appl Microbiol 1965; 13: 762–5.

● 54. Maneke E, Pridmore A, Goby L, Lang I. Kill rate of mastitis pathogens by a combination of cefalexin and kanamycin. J Appl Microbiol 2011;110:184–90.

● 55. Sérieys F, Raguet Y, Goby L, Schmidt H, Friton G. Comparative efficacy of local and sys- temic antibiotic treatment in lactating cows with clinical mastitis. J Dairy Sci 2005; 88: 93–9.

● 56. Fuenzalida M, Ruegg P. Negatively con- trolled, randomized clinical trial to evaluate use of intramammary ceftiofur for treatment of nonsevere culture-negative clinical mastitis. J Dairy Sci 2019; 102: 3321–38.

● 57. Fogsgaard K, Løvendahl P, Bennedsgaard T, Østergaard S. Changes in milk yield, lactate de- hydrogenase, milking frequency, and interquarter yield ratio persist for up to 8 weeks after antibi- otic treatment of mastitis. J Dairy Sci 2015; 98: 7686–98.

● 58. Bogin E , Ziv G, Avidar J. Enzyme-activities in normal and inflamed bovine udder tissues. Zentral- blatt für Veterinärmedizin Reihe 1976; 23: 460–66.

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Published

2023-01-26

How to Cite

El-Neshwy, W., Abo-Zaid, A.A., El-Mekawey, M.F, & Soliman, A.H. (2023). PREVALENCE, ANTIMICROBIAL RESISTANCE PROFILES OF CLINICAL AND SUBCLINICAL MASTITIS IN LACTATING COW WITH ASSESSMENT OF TREATMENT TRIAL. Slovenian Veterinary Research, 60(25-Suppl), 327–39. https://doi.org/10.26873/SVR-1618-2022

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Veterinary Medicine and The One Health Concept