GENOTYPIC CHARACTERIZATION OF SOME PATHOGENIC FUNGI ISOLATED FROM POULTRY AND THEIR SURROUNDINGS
DOI:
https://doi.org/10.26873/SVR-1603-2022Keywords:
phenotypic, genotypic, molecular, fungi, poultryAbstract
Regarding high morbidity, mortality, and production losses, fungi infections have their importance among infectious illnesses and seem to be one of the main challenges facing poultry producers. This study aims to identify the genotypic characteristics of some fungi isolated from poultry. To reach this end, in El-Gharbia Governorate, Egypt, a total of 210 birds with a history of respiratory distress were randomly selected from a variety of private farms and hatcheries. The birds were sacrificed; tissue pieces were collected. In addition, a total of 87 samples of the poultry surroundings including 40 samples of poultry ration, 14 bedding materials, 4 air samples, and 29 water samples were collected. Using traditional fungal isolation, four fungal species were recovered, namely, Aspergillus niger, Aspergillus flavus, Cladosporium perangustum, and Penicillium chrysogenum. PCR was performed by fungus-specific universal primer pairs (ITS1 and ITS4) to identify and describe the genotype of isolated fungi. All examined isolates' ITS1-5.8SrDNA regions could be amplified. A purified PCR product was sequenced according to the Emerald Amp GT PCR master mix. This was initially performed to establish sequence identity to GenBank accession numbers. The rRNA gene for 5.8 sRNA divides the two ITS sections, which are situated between the 18S and 28S rRNA genes. ITS-1 gene sequence of the isolated Cladosporium perangustum (GeneBank accession number was OM 407392). The Sequence of the ITS-1 of isolated Penicillium chrysogenum (GeneBank accession numbers for studied nucleotide sequences were OM407401; OM407402; OM403685, and OM403686). For the examined nucleotide sequences, the GeneBank accession number for the ITS-1 internal transcribed spacer region of single Aspergillus niger was OM407391. GeneBank accession numbers for the isolated Aspergillus flavus ITS-1 sequence examined nucleotide sequences were OM403676, OM403677, and OM403678. In conclusion, genotypic characterization confirmed the phenotypic traditional fungal identification in the present study. Aspergillus species are the major fungi associated with birds in Egypt farms. The predominantly identified species were Aspergillus flavus and Penicillium chrysogenum.
References
● 1. Fahmy NE, Eltholth MM, Mohamed RA, et al. Management of Poultry Farms and the Likelihood of Contamination of Poultry Feed with Mycotoxins in Gharbia Governorate, Egypt. World Vet J 2015; 5(4): 51–8.
● 2. Kusi LY, Agbeblewu S, Anim, IK, Nyarku KM, The challenges and prospects of the commercial poultry industry in Ghana: Inter J Mang Sci 2015; 5(6): 476–89.
● 3. Asfaw M, Dawit D. Review on the major fungal disease of poultry. Br J Poult Sci 2017;(6): 16–25.
● 4. Abbas MS, Yassein SN& Khalaf JM. Isolation and identification of some important mycological isolates from dropping of birds in Baghdad. J Entomol Zool Stud 2017; 5(53): 671–3.
● 5. Dhama K, Chakraborty S, Wani MY, et al. Novel and emerging therapies safeguarding the health of humans and their companion animals: a review. Pakistan J Biol Sci 2013; 16(3): 101–11.
● 6. Wang DY. Genetic diversity and antifungal susceptibility of Aspergillus spp. isolates from avian farms in Guangxi, China (Doctoral dissertation, AgroParisTech; Université de Guangxi (China); A thesis, 2012.
● 7. Abd El Tawab AA, Maarouf AA, El-Hofy FI, Ahmed K. Molecular characterization of some fungi isolated from broiler chicken farms. Benha Vet Med J 2015; 29(2): 106–18.
● 8. Arné P, Lee MD. Fungal infections. Diseases of poultry 14th Edition chapter 25, 2020; 1109–33.
● 9. Yousif J, Abdalla ME, Eltayeb F. Isolation of Aspergillus flavus From Poultry Feed in Khartoum State. Sudan J Sci Technol 2021; 22(1): 28–35.
● 10. Della VR, Hevia A, Vivot W, Fernández J, Córdoba SB & Reynaldi FJ. Aspergillosis in domestic and wild birds from Argentina. Braz J Veterinary Res Anim Sci 2019; 56(2): e152460.
● 11. Thathana MG, Murage H, Abia ALK, Pillay M. Morphological characterization and determination of aflatoxin-production potentials of Aspergillus flavus isolated from maize and soil in Kenya. Agriculture 2017; 7(10): 80.
● 12. Hariharan G, Prasannath K. Recent advances in molecular diagnostics of fungal plant pathogens: a mini-review. Front Cell Infect Microbiol 2021; 10: 829.
● 13. Kidd SE, Chen SC-A, Meyer W, Halliday CL. A New Age in Molecular Diagnostics for Invasive Fungal Disease: Are We Ready? Front. Microbiol 2020; 10: 2903.
● 14. Collins L. Microbiological method,5th Ed. Microbiology laboratory manuals. British Liberary Butter Worth in Co. 2002.
● 15. Luo G, Mitchell TG. Rapid Identification of Pathogenic Fungi Directly from Cultures by Using Multiplex PCR. J Clin Microbiol 2002; 2860–5.
● 16. Sambrook J, Fritscgh EF. Molecular cloning. A laboratory Manual. Cold Spring Harbor Laboratory Press 1989; New York.
● 17. Thompson JD, Higgins DG, Gibson TJ. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice. Nucleic Acids Research 1994; 22(22): 4673–80.
● 18. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; (30): 2725–2729.
● 19. Ostović M, Ravić I, Kovačić M, et al. Differences in fungal contamination of broiler litter between summer and winter fattening periods. Arh Hig Rada Toksikol 2021; (72): 140–7.
● 20-Sugui JA, Kwon-Chung KJ, Juvvadi PR, et al. Aspergillus fumigatus and related species. Cold Spring Harbor perspectives in medicine 2015; 5(2): a019786.
● 21. Krulj J, Ćurčıć N, Stančıć AB, et al. Molecular Identification and Characterization of Aspergillus flavus Isolates Originating from Serbian Wheat Grains. Acta Alimentaria 2020; 49(4): 382–9.
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