• Sawsan El Basuni Avian and Rabbit Diseases Department, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt, *Corresponding author, E-mail:
  • Mai Osman Biochemistry, Toxicology and Nutritional Deficiency Diseases Department, Animal Health Research Institute, Benha Branch, Egypt
  • Reem Soliman Evaluation of Inactivated Viral Poultry Vaccines Department, Central Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center (ARC)
  • Yasmeen Magdy Department of Anatomy, Faculty of Veterinary M edicine, Benha University, Benha 13736, Egypt
  • El-Hadary Abdalla Biochemistry Department, Faculty of Agriculture, Benha University, Benha 13736, Egypt
  • Reda Fathy Avian and Rabbit Diseases Department, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt



Newcastle disease, broilers, Azadirachta Indica, Neem, methanolic extract, antiviral


Newcastle disease (ND) is an important viral disease that threatens the global poultry industry. The urgent need for a safe and effective antiviral alternative prompted us to evaluate neem methanolic leaf extract against Newcastle disease virus (NDv) in broilers. A total of Sixty Cobb chicks (day old) were allocated randomly into 4 equal groups; group A was a control negative, and group B was a control positive. Groups C and D received a methanolic neem leaf extract (MNLE) in drinking water for five days at 500 and 1000 µg/kg BW, respectively. At 25th days old, chicks in groups B, C and D were challenged via the oculonasal route with 0.2 ml of virulent NDv. The use of MNLE in groups C and D significantly reduced morbidity and mortality as well as the macroscopic and microscopic scoring lesions of all examined organs compared to the infected untreated group B. The high dose of MNLE (1000 µg/ kg BW; group D) was more efficient and significant in relieving the clinical and pathological abnormalities caused by ND challenge compared with the low dose (500 µg/ kg BW; group C).
Moreover, positive effects of the herbal treatment on the experimentally ND-infected chickens were evidenced by reducing viral RNA concentrations in the oropharyngeal swabs at 3, 5, and 7 days post challenge and the infectivity titer of the virus isolated from the trachea, spleen, and cecal tonsils at 7 days post challenge. In conclusion, MNLE possesses potential antioxidant to have a power to control NDv shedding and infection. So, MNLE could be a source of inspiration for new alternative antivirals.


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Veterinary Medicine and The One Health Concept