THE EFFECTS OF DIETARY EUBIOTICS OR INTRAVENOUS AMINO ACID INFUSIONS ON NUTRIENT DIGESTIBILITY, RUMEN FERMENTATION, PERFORMANCE AND BLOOD PARAMETERS OF BUFFALO CALVES UNDER SUBTROPICAL CLIMATIC CONDITIONS
DOI:
https://doi.org/10.26873/SVR-1588-2022Keywords:
eubiotics, amino acids, buffalo calves, nutrient digestibility, rumen fermentation, growth performanceAbstract
This study was conducted to compare the effects of dietary eubiotics or intravenous amino acid infusions (IVAAI) as two different growth promoters on nutrient digestibility, rumen fermentation, performance, and blood biochemical parameters of buffalo calves in subtropical climatic conditions. Thirty male buffalo calves (284.40 ± 18.45 kg) were randomly distributed into three groups and fed a basal diet (BD) of concentrate feed mixture and roughages. The first group was fed BD and considered as the control, the second group was fed the BD supplemented with eubiotics at 1.0 kg/ton of concentrate, whereas the third group was intravenously infused with amino acid (IVAAI) injection at a dose of 2.0 ml/100 kg body weight. Results showed that the total gain and the average daily gain were improved (P < 0.05) with dietary eubiotics. The digestibility of some nutrients was increased (P < 0.05) with dietary addition of eubiotics. In addition, eubiotics stabilize (P < 0.05) the rumen pH, which reduce the risk of subacute ruminal acidosis but increased (P < 0.05) ruminal NH3-N and total volatile fatty acids. The rectal temperature was decreased (P < 0.05) with eubiotics supplementation. In conclusion, the use of eubiotics induced superior positive effects on the digestibility of nutrients, rumen fermentation, rumen enzymes, rumen protein concentration, growth performance, feed conversion, blood parameters and ameliorated the harmful effects of thermal stress of buffalo calves in comparison with intravenous infusion of amino acids.
Key words: eubiotics; amino acids; buffalo calves; nutrient digestibility; rumen fermentation; growth performance
References
● 1. Wierup M. The control of microbial diseases in animals: alternatives to the use of antibiotics. International Journal of Antimicrobial Agents. 2000; 14 (4): 315–9.
● 2. Butaye P, Devriese LA, Haesebrouck F. Antimicrobial growth promoters used in animal feed: effects of less well known antibiotics on gram-positive bacteria. Clinical microbiology reviews. 2003; 16 (2): 175–88.
● 3. Seo JK, Kim SW, Kim MH, Upadhaya SD, Kam DK, Ha JK. Direct-fed microbials for ruminant animals. Asian-Australasian Journal of Animal Sciences 2010; 23 (12): 1657–67.
● 4. Abd El Tawab AM, Hassan AA, Khattab MS, Matloup OH, Farahat ES, Khalel MS, Morsy TA, Fouad MT. Productive performance of lactating frisian cows fed sugar beet leaves silage treated with lactic acid bacteria. Int. J. Zool. Res. 2017; 13:74–82.
● 5. Nowak P, Kasprowicz-Potocka M, Zaworska A, Nowak W, Stefańska B, Sip A, Grajek W, Juzwa W, Taciak M, Barszcz M, Tuśnio A. The effect of eubiotic feed additives on the performance of growing pigs and the activity of intestinal microflora. Archives of animal nutrition 2017; 71 (6):455–69.
● 6. Wang Y, McAllister TA, Rode LM, Beauchemin KA, Morgavi DP, Nsereko VL, Iwaasa AD, Yang W. Effects of an exogenous enzyme preparation on microbial protein synthesis, enzyme activity and attachment to feed in the Rumen Simulation Technique (Rusitec). British Journal of Nutrition 2001; 85 (3): 325–32.
● 7. Wolfe RR. Regulation of muscle protein by amino acids. The Journal of Nutrition 2002; 132 (10): 3219S-24S.
● 8. NRC. National Research Council. Nutrient requirements of dairy cattle: 2001. National Academies Press;Washington, D.C., 2001
● 9. Mader TL, Davis MS, Brown-Brandl T. Environmental factors influencing heat stress in feedlot cattle. Journal of Animal Science. 2006; 84 (3): 712–9.
● 10. AOAC. Official method of Analysis. 18th Edition, Association of Officiating Analytical Chemists, Washington DC, 2005.
● 11. Goering HK and Van Soest PJ. Forage Fiber Analysis (Apparatus Reagents, Procedures and Some Applications). Agriculture Handbook. United States Department of Agriculture, Washington DC, 1970.
● 12. Williams CH, David DJ, Iismaa O. The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. The Journal of Agricultural Science 1962; 59 (3): 381.
● 13. Maynard LA, Loosli JK. Animal nutrition. 6th eds., McGraw-Hill, New York, USA, 1969.
● 14. Conway EJ. Microdiffusion analysis and volumetric error. (5th. Ed.) Crosby- Lockwood and Sons Ltd., London 1947: 90.
● 15. Warner ACI. Production of volatile fatty acids in the rumen. Methods of measurement. Nutrition Abstract Review 1964; 34: 346–99.
● 16. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical chemistry. Analytical Chemistry 1959; 31: 426–8.
● 17. Peled N, Krenz MC. A new assay of microbial lipases with emulsified trioleoyl glycerol. Analytical Biochemistry 1981; 112: 219–22.
● 18. Folin O, Ciocalteu V. On tyrosine and tryptophane determinations in proteins. The Journal of Biological Chemistry 1927; 73 (2): 627–50.
● 19. Weatherburn MW. Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry 1967; 39 (8): 971–4.
● 20. Lowry OH. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry1951; 193: 265–75.
● 21. SAS. SAS/STAT Guide for personal computer. Version 8.2 ed Cary (NC): SAS INST 2002.
● 22. Steel RG, Torrie JH. Duncan's new multiple range test. Principles and procedures of statistics 1980: 187–8.
● 23. Kritas SK, Govaris A, Christodoulopoulos G, Burriel AR. Effect of Bacillus licheniformis and Bacillus subtilis supplementation of ewe's feed on sheep milk production and young lamb mortality. Journal of Veterinary Medicine Series A 2006; 53(4): 170–3.
● 24. Sallam SM, Kholif AE, Amin KA, El-Din ANN, Attia MF, Matloup OH and Anele UY. Effects of microbial feed additives on feed utilization and growth performance in growing Barki lambs fed diet based on peanut hay. Animal Biotechnology 2020; 31 (5): 447–54.
● 25. Schei I, Danfær A, Boman IA, Volden H. Post-ruminal or intravenous infusions of carbohydrates or amino acids to dairy cows 1. Early lactation. Animal 2007; 1 (4): 501–14.
● 26. Kassube KR, Kaufman JD, Pohler KG, McFadden JW, Ríus AG. Jugular-infused methionine, lysine and branched-chain amino acids does not improve milk production in Holstein cows experiencing heat stress. Animal 2017; 11 (12): 2220–8.
● 27. Klemesrud MJ, Klopfenstein TJ, Stock RA, Lewis AJ, Herold D.W. Effect of dietary concentration of metabolizable lysine on finishing cattle performance. Journal of Animal Science 2000; 78 (4): 1060–6.
● 28. Zhou, Z, Bulgari O., Vailati-Riboni, M, Trevisi E, Ballou MA, Cardoso FC, Luchini DN Loor J.J. Rumen-protected methionine compared with rumen-protected choline improves immunometabolic status in dairy cows during the peripartal period. Journal of Dairy Science 2016; 99 (11): 8956–69.
● 29. Robinson PH, Chalupa W, Sniffen CJ, Julien WE, Sato H, Fujieda T, Ueda T, Suzuki, H. Influence of abomasal infusion of high levels of lysine or methionine, or both, on ruminal fermentation, eating behavior, and performance of lactating dairy cows. Journal of Animal Science 2000; 78 (4): 1067–77.
● 30. Jo JH, Lee JS, Ghassemi Nejad J, Kim WS, Moon JO, Lee HG. Effects of Dietary Supplementation of Acetate and L-Tryptophan Conjugated Bypass Amino Acid on Productivity of Pre-and Post-Partum Dairy Cows and Their Offspring. Animals 2021; 11 (6): 1726.
● 31. Mazinani M, Naserian AA, Rude BJ, Tahmasbi AM, Valizadeh R. Effects of feeding rumen–protected amino acids on the performance of feedlot calves. Journal of Advanced Veterinary and Animal Research 2020; 7 (2): 229–33.
● 32. Wu G. Amino acids: biochemistry and nutrition. CRC Press, Boca Raton, FL, USA, 2013.
● 33. Shwartz G, Rhoads ML, VanBaale MJ, Rhoads RP, Baumgard LH. Effects of a supplemental yeast culture on heat-stressed lactating Holstein cows. Journal of Dairy Science, 2009; 92 (3): 935–42.
● 34. Purwar V, Oberoi PS, Dang AK. Effect of feed supplement and additives on stress mitigation in Karan Fries heifers. Veterinary world 2017; 10 (12): 1407–12.
● 35. Ríus AG, Kaufman JD, Li MM, Hanigan MD, Ipharraguerre IR. Physiological responses of Holstein calves to heat stress and dietary supplementation with a postbiotic from Aspergillus oryzae. Scientific reports 2022; 12 (1):1–10.
● 36. Yamaoka I, Doi M, Nakayama M, Ozeki, A., Mochizuki, S, Sugahara, K , Yoshizawa F. Intravenous administration of amino acids during anesthesia stimulates muscle protein synthesis and heat accumulation in the body. American Journal of Physiology-Endocrinology and Metabolism 2006; 290 (5): E882–8.
● 37. Khan RU, Shabana N, Kuldeep D, Karthik K, Ruchi T, Abdelrahman MM, Alhidary IA Arshad, Z. Direct-fed microbial: beneficial applications, modes of action and prospects as a safe tool for enhancing ruminant production and safeguarding health. International Journal of Pharmacology 2016; 12 (3): 220–31.
● 38. Kholif AE, Kassab AY, Azzaz HH, Matloup OH, Hamdon HA, Olafadehan OA, Morsy TA, Essential oils blend with a newly developed enzyme cocktail works synergistically to enhance feed utilization and milk production of Farafra ewes in the subtropics. Small Ruminant Research 2018; 161:43–50.
● 39. Ali CS, Sharif M, Nisa M, Javaid A., Hashmi N, Sarwar M. Supplementation of ruminally protected proteins and amino acids: feed consumption, digestion and performance of cattle and sheep. International Journal of Agriculture and Biology 2009; 11 (4): 477–82.
● 40. Movaliya JK, Dutta KS, Savsani HH, Gadariya MR, Murthy KS, Bhadaniya AR. Growth performance, nutrient utilization and economics of feeding bypass methionine and lysine in Jaffrabadi heifers. Indian J Anim Nutr 2013; 30 (2): 124–7.
● 41. Qadis AQ, Goya S, Ikuta K, Yatsu M, Kimura A, Nakanishi S, Sato S. Effects of a bacteria-based probiotic on ruminal pH, volatile fatty acids, and bacterial flora of Holstein calves. Journal of Veterinary Medical Science 2014; 6 (6): 877–85.
● 42. Mohamed MI, Maareck YA, Abdel-Magid SS, Awadalla IM. Feed intake, digestibility, rumen fermentation and growth performance of camels fed diets supplemented with a yeast culture or zinc bacitracin. Animal Feed Science and Technology 2009; 149 (3-4): 341–5.
● 43. El-Waziry AM, Ibrahim HR. Effect of Saccharomyces cerevisiae of yeast on fiber digestion in sheep fed berseem (Trifolium alexandrinum) hay and cellulase activity. Australian Journal of Basic and Applied Sciences 2007; 1 (4): 379–85.
● 44. Uyeno Y, Shigemori S, Shimosato T. Effect of probiotics/prebiotics on cattle health and productivity. Microbes and environments 2015; 30 (2): 126–32.
● 45. Plaizier JC, Krause DO, Gozho GN, McBride BW. Subacute ruminal acidosis in dairy cows: the physiological causes, incidence and consequences. The Veterinary Journal 2008; 176 (1): 21–31.
● 46. Van E. Nolte J, Loest CA, Ferreira AV, Waggoner JW, Mathis CP. Limiting amino acids for growing lambs fed a diet low in ruminally undegradable protein. Journal of animal science. 2008; 86 (10): 2627-41.
● 47. Kiran RR, Kumar DS. Influence of yeast culture supplementation on rumen fermentation of bulls fed complete rations. Int J Agric Sci Vet Med 2013; 1: 8–15.
● 48. Russell, JB, O'connor JD, Fox DG, Van Soest PJ, Sniffen CJ. A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. Journal of animal science 1992; 70 (11): 3551–61.
● 49. Agarwal N, Kamra DN, Chaudhary LC, Agarwal, I, Sahoo A., Pathak NN. Microbial status and rumen enzyme profile of crossbred calves fed on different microbial feed additives. Letters in Applied Microbiology 2002; 34 (5): 329–36.
● 50. Zhao S, Min L, Zheng N, Wang J. Effect of heat stress on bacterial composition and metabolism in the rumen of lactating dairy cows. Animals 2019; 9 (11): 925.
● 51. Hristov AN, McAllister TA, Cheng KJ. Intraruminal supplementation with increasing levels of exogenous polysaccharide-degrading enzymes: effects on nutrient digestion in cattle fed a barley grain diet. Journal of Animal Science 2000; 78 (2): 477–87.
● 52. Yang WZ, Beauchemin KA, Rode LM. A comparison of methods of adding fibrolytic enzymes to lactating cow diets. Journal of Dairy Science 2000; 83 (11): 2512–20.
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