• Soad M. Menshawy Department of Parasitology, Faculty of Veterinary Medicine, Damanhour University, 22511 Damanhour, Elbehera, Egyp
  • Bothaina H. Essa Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Damanhour University, 22511 Damanhour, Elbehera, Egypt
  • Sabah I. Shaaban Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Damanhour University, 22511 Damanhour, Elbehera, Egypt
  • Mohamed Hamada Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Sadat City, 32511 Sadat City, Menofia, Egypt
  • Sahar F. Mahmoud Department of Histology and Cytology, Faculty of Veterinary Medicine, Damanhour University, 22511 Damanhour, Elbehera, Egypt
  • Mahmoud AbouLaila Department of Parasitology, Faculty of Veterinary Medicine, Damanhour University, 22511 Damanhour, Elbehera, Egypt, Corresponding author, E-mail:
  • Shimaa S. Sorour Department of Parasitology, Faculty of Veterinary Medicine, Kafrelsheikh University, 335116 Kafrelsheikh, Kafrelsheikh, Egypt



Sarcocystis fusiformis, prevalence, genetic characterization, Buffalo, Elbehera, Kafrelsheik


Sarcocystosis considerably occurs in a wide host range including animals, reptiles, humans, and birds. This study was conducted to determine the prevalence of Sarcocystis spp. using abattoir inspection, genetic characterization, as well as histopathology in water buffaloes in two provinces, Elbehera and Kafrelsheikh, Egypt. Specimens were collected from esophagus, tongue, and masseters of 400 slaughtered buffaloes in Elbehera (n= 215) and Kafrelsheikh (n= 185). Samples were examined macroscopically and histopathologically. Furthermore, genetic characterization of Sarcocystis spp. was performed using the 18SrRNA gene-based PCR. The total prevalence was 71.0% (75.3% and 65.9% in Elbehera and Kafrelsheikh, respectively). Aged buffaloes had a higher prevalence than young ones. Females had a higher prevalence than males. The esophagus was the most infected organ. Molecular analysis revealed that the recovered species was S. fusiformis. This is the first genetic characterization of S. fusiformis in water buffaloes from Elbehera and Kafrelsheikh, Egypt. Higher prevalence proposed the potential role of cats in the transmission of S. fusiformis, which, in turn, requires strict hygienic measures to protect animals and humans from infection.


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How to Cite

Menshawy, S. M., Essa, B. H., Shaaban, S. I., Hamada, M., Mahmoud, S. F., AbouLaila, M., & Sorour, S. S. (2023). PREVALENCE AND GENETIC CHARACTERIZATION OF Sarcocystis fusiformis IN WATER BUFFALOES (Bubalus bubalis) IN TWO NORTHERN PROVINCES OF EGYPT. SLOVENIAN VETERINARY RESEARCH, 60(25-Suppl), 195–203.



Veterinary Medicine and The One Health Concept