• Isayed I. Hassanein Department of Nutrition and Clinical Nutrition, Zagazig University, 44511, Zagazig, Sharkia, Egypt
  • Abdallah E. Metwally Department of Nutrition and Clinical Nutrition, Zagazig University, 44511, Zagazig, Sharkia, Egypt
  • Hossam Eldin M. Abd Elbaky Department of Nutrition and Clinical Nutrition, Zagazig University, 44511, Zagazig, Sharkia, Egypt, Corresponding author, E-mail: vet7ossam@gmail.com




broiler, nucleotide, PUFA oil sources, growth performance, immunity, gut morphology and economic efficiency


This study examined the effects of nucleotide supplementation on broiler chick growth performance, immunological response, carcass traits, meat quality, serum biochemical parameters, total antioxidant capacity, intestinal morphology, mortality rate, and economic efficiency measures of broiler chicks. A total of 180 one-day-old chicks (Ross 308) were distributed into 6 groups, each of which consisted of 30 chicks, and each group was divided into 3 replicates so that each replicate contained 10 chicks fed on six experimental diets as follows:  basal diet without oil or nucleotide (T1), basal diet with 1% soybean oil and without nucleotide(T2), 50 grams nucleotide per 100-kilogram diet with different fat sources (no oil(T3), 1% soybean oil(T4), 1% linseed oil(T5) &1% fish oil(T6) respectively during the experimental period (5 weeks). Growth performance parameters were detected per stage period. Four chicks from each replicate were used at the termination of the experiment for analysis of the above mentioned measurements. Results revealed that supplementation of nucleotide in diets of broiler chicks improved feed conversion ratio, carcass traits, intestinal morphology, serum biochemical parameters, immunological response, bursa of Fabricius weight, and the best ratios were observed in groups fed nucleotide in combination with fish and linseed oil. Also, there was an increased economic efficiency in the SBO fed group(T2) then group fed nucleotides in mix with linseed oil (T5) and control (T1). In comparison to the control groups (T1 & T2), groups fed nucleotides and PUFA oil sources had significantly lower n-6: n-3 ratio in breast muscle, and mortality rate.


● 1. Ravindran V. Poultry feed availability and nutrition in developing countries. Poul Develop Rev 2013 Jan; 2: 60–63.

● 2. Doppenberg J, Van der Aar PJ, editors. Facts about fats: a review of the feeding value of fats and oils in feeds for swine and poultry. 2017. A book.

● 3. Gogus U, Smith C. n‐3 Omega fatty acids: a review of current knowledge. Inter J Food Sci Technol 2010 Mar; 45(3):417–36.

● 4. Prakash S, Palod J, Sharma RK, Singh SK. Effect of graded levels of nucleotide supplementation on certain serum biochemical parameters in Japanese quails. Ind J Anim Res 2017 Feb 1; 51(1):93–6.

● 5. Jung B, Batal AB. Effect of dietary nucleotide supplementation on performance and development of the gastrointestinal tract of broilers. British Poul Sci 2012 Feb 1; 53(1):98–105.

● 6. Salah M, Suprijatna E, Djauhari ML, et al. The effects of nucleotide supplementation on the productivity, immune response and meat quality of broiler chicken reared under different environmental conditions. Livestock Res Rural Develop 2019; 31(11):174.

● 7. Rutz F, Xavier EG, Anciuti MA, et al. The role of nucleotides in improving broiler pre-starter diets. Gut efficiency; the key ingredient in pig and poultry production. 2008 Jan 28: 155–65.

● 8. Esteve-Garcia E, Martinez-Puig D, Borda E, et al. Efficacy of a nucleotide preparation in broiler chickens. InProceedings 16th European Symposium on Poultry Nutrition. Strasbourg, France 2007 Aug 26 (pp. 511–4).

● 9. Wu C, Yang Z, Song C, et al. Effects of dietary yeast nucleotides supplementation on intestinal barrier function, intestinal microbiota, and humoral immunity in specific pathogen-free chickens. Poul Sci 2018 Nov 1; 97(11):3837–46.

● 10. Raheel IA, Orabi A, Hala SH, Abed AH, Fouad IA, Refaat M. Immune-modulating effects of Aviboost® nucleotides on the intestinal epithelium of broiler chickens. Inter J Vet Sci 2019; 8(2):89–95.

● 11. ChioFalo B, Presti VL, Samoini G, Chiofalo V, Liotta L. Nucleotides in broiler chicken diet: effect on breast muscles quality. Czech J Food Sci 2011 Aug 10; 29(4):308–7.

● 12. Pelícia VC, Sartori JR, Zavarize KC, et al. Effect of nucleotides on broiler performance and carcass yield. Braz J Poul Sci 2010; 12:31–4.

● 13. Aviagen. Ross 308 Broiler Nutrition Specifications. Aviagen Ltd., Newbridge, UK. 2019.

● 14. Janssen WM. European table of energy values for poultry feedstuffs. Spelderholt Center for Poultry Research and Information Services, Beekbergen, Netherlands. 1989.

● 15. Brake J, Havenstein GB, Scheideler SE, et al. Relationship of sex, age, and body weight to broiler carcass yield and offal production. Poul Sci 1993 Jun 1; 72(6):1137–45.

● 16. Anon A. Methods for examination poultry biologics and for identifying & quantifying a vian pathogens. Natl. Acad. Sci. Washington, DC. 1971:1–184.

● 17. Wahlefeld AW. Triglycerides determination after enzymatic hydrolysis. In Methods of enzymatic analysis 1974 Jan 1 (pp. 1831–5). Academic Press.

● 18. Naito HK, Kaplan AQ. High-density lipoprotein (HDL) cholesterol. Clin. Chem. Toronto. Princeton. 1984:1207–13.

● 19. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972 Jun 1; 18(6):499–502.

● 20. Burstein M, Scholnick HR. Lipoprotein-polyanion-metal interactions. Advances in lipid research. Elsevier. 1973 Jan 1; 11:67–108.

● 21. Grant GH, Silverman LM, Christenson RH. Amino acids and proteins. Fundamentals of clinical chemistry, 3rd edn. WB Saunders, Philadelphia, 1987; pp 291–345

● 22. Doumas BT, Watson WA, Biggs HG. Albumin standards and the measurement of serum albumin with bromcresol green. Clinica chimica acta 1971 Jan 1; 31(1):87–96.

● 23. Doumas BT, Biggs HG. Standard methods of clinical chemistry. Academic Press, Chicago. 1972; 7:175-89.

● 24. Koracevic D, Koracevic G, Djordjevic V, et al. Method for the measurement of antioxidant activity in human fluids. Journal of clinical pathology. 2001 May 1; 54(5): 356–61.

● 25. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J biol Chem 1957 May 1; 226(1):497–509.

● 26. Ichihara KI, Fukubayashi Y. Preparation of fatty acid methyl esters for gas-liquid chromatography [S]. J Lipid Res 2010 Mar 1; 51(3): 635–40.

● 27. Drury RA, Wallington EA, Cameron R. Carleto’s histological technique 4th ed Oxford university press, New York. And Toronto. 1967; pp 151, and pp 242–5

● 28. El-Kerdawy DM. Olive pulp as a new energy source for growing rabbits. Egyptian J Rabbits Sci 1997; 7:1-2.

● 29. Snedecor GW, Cochran WG. Statistical methods. 8th edn East West Press Pvt. Ltd., New Delhi, India. 1994; 313.

● 30. Gunstone FD. Fatty acids—Nomenclature, structure, isolation and structure determination, biosynthesis and chemical synthesis. InFatty acid and lipid chemistry. 1996 (pp.1–34). Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4131-8

● 31. Saleh H, Rahimi SH, Karimi TM. The effect of diet that contained fish oil on performance, serum parameters, the immune system and the fatty acid composition of meat in broilers. Iran J Vet Med 2009; 3(2): 69–75.

● 32. Jameel YJ, Sahib AM. Study of some blood parameters of broilers fed on ration containing fish oil. J Biol Agric Healthc 2014; 4(7): 67–71.

● 33. Fonia N, Singh CB, Singh DV, et al. Effect of nucleotides supplementation on carcass traits and meat composition of thigh and breast muscles of broiler chicken. Ind J Poul Sci 2019; 54(3):213–6.

● 34. MSM Nafees MP. Dietary enrichment of broiler chicken with omega-3 fatty acids and beneficial role in human cardiovascular health: A Review. AgriEast 2015; 10: 27–35

● 35. Prakash S, Palod J, SharmaR K, et al. Impact of Dietary Nucleotides on the Production Traits of Japanese Quails. Inter J Sci Environ Technol 2016;5(6):4622–9.

● 36. Kumar PV, Natarajan A. Effect of nucleotide through feed on body weight and blood biochemical parameters in broiler chickens. Pharma Innov J 2021; SP-10(2): 170–3.

● 37. Attia YA, Al-Harthi MA, Abo El-Maaty HM. The effects of different oil sources on performance, digestive enzymes, carcass traits, biochemical, immunological, antioxidant, and morphometric responses of broiler chicks. Fron Vet Sci 2020 Apr 28; 7:181.

● 38. Hassanein EI, Metwally AE, Abd Elbaky HE. Effects of dietary fish oil supplementation in the diet on performance of Broiler chicks. J Univ Shanghai Sci Technol 2021; pp: 56–69.

● 39. Qi KK, Chen JL, Zhao GP, et al. Effect of dietary ω6/ω3 on growth performance, carcass traits, meat quality and fatty acid profiles of Beijing‐you chicken. J Anim Physiol Anim Nutr 2010 Aug; 94(4):474–85.

● 40. Ibrahim D, El-Sayed R, Khater SI, et al. Changing dietary n-6: n-3 ratio using different oil sources affects performance, behavior, cytokines mRNA expression and meat fatty acid profile of broiler chickens. Anim Nutr 2018 Mar 1; 4(1):44–51.

● 41. Daneshmand A, Kermanshahi H, Mesgaran MD, et al. Combination of purine and pyrimidine nucleosides influences growth performance, gut morphology, digestive enzymes, serum biochemical indices and immune functions in broiler chickens. Anim Feed Sci Technol 2017 Jun 1; 228:186–93.

● 42. Kruger D, Werf MV. Benefits of nucleotide supplementation in poultry. Ohly Appl Note 2018; 1–3.

● 43. Hess JR, Greenberg NA. The role of nucleotides in the immune and gastrointestinal systems: potential clinical applications. Nutr Clin Pract 2012 Apr; 27(2):281–94.

● 44. Khedr N, Ahmed T. Effect of dietary nucleotide supplementation on broiler intestinal histomorphology. Benha Vet Med J 2020 Dec 1; 39(2):127–31.

● 45. Aziza AE, Awadin WF, Quezada N, et al. Gastrointestinal morphology, fatty acid profile, and production performance of broiler chickens fed camelina meal or fish oil. Eur J Lipid Sci Technol 2014 Dec; 116(12):1727–33.

● 46. Zahran RH, Khader NE, Ahmed TE. Effect of dietary nucleotide supplementation on broiler performance and economic efficiency. Benha Vet Med J 2020 Sep 1;39(1):34–9.

● 47. Ahiwe EU, Abdallh ME, et al. Influence of dietary supplementation of autolyzed whole yeast and yeast cell wall products on broiler chickens. Asian-Australasian J Anim Sci 2020 Apr; 33(4):579–87.

● 48. Fathi MM, Al-Mansour S, Al-Homidan A, et al. Effect of yeast culture supplementation on carcass yield and humoral immune response of broiler chicks. Vet World 2012 Nov 1; 5(11):651–7.




How to Cite

Hassanein, I. I., Metwally, A. E., & Abd Elbaky, H. E. M. (2023). EFFECTS OF DIETARY NUCLEOTIDES SUPPLEMENTATION ON THE PERFOR-MANCE OF BROILER CHICKS. Slovenian Veterinary Research, 60(25-Suppl), 159–71. https://doi.org/10.26873/SVR-1575-2022



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