THE EFFECT OF CRUDE Nigella sativa OIL AGAINST THE ACUTE TOXICITY OF DICLOFENAC SODIUM AND IBUPROFEN ON THE LIVERS OF ALBINO MICE

Malik Husna, Sajjad Sumera, Saeed Laiba, Asghar Anam

Abstract


The present research work is an investigation of the effect of Nigella sativa oil against the acute toxicity induced by the drugs diclofenac and ibuprofen on the liver enzymes, body weight and Hepatosomatic index of Swiss albino mice. Thirty-six healthy, adult Swiss albino mice were used to assess diclofenac- and ibuprofen-induced hepatotoxicity and the hepatoprotective effect of N. sativa oil. The animals were divided into the control group and five experimental groups. The animals in the control group were given saline (0.9 percent of NaCl) only, whereas the animals in experimental groups were given single sub-lethal doses of diclofenac, ibuprofen and N. sativa oil alone and together. A significant (p<0.05) reduction in the body weight of the diclofenac- and ibuprofen-treated groups was recorded. The hepatosomatic index showed significant (p<0.05) changes in the combined treated groups. Hepatotoxicity can be confirmed by comparing the significant (p<0.05) and highly significant (p<0.01) increase in
liver enzymes in all the treated animals. The hepatoprotective effect of N. sativa oil was confirmed.

Key words: diclofenac; ibuprofen; hepatotoxicity; hepatoprotective; N. sativa oil

 

VPLIV OLJA ČRNE KUMINE (Nigella sativa) NA AKUTNO ZASTRUPITEV JETER, POVZROČENO Z NATRIJEVIM DIKLOFENAKOM IN IBUPROFENOM, PRI ALBINO MIŠIH 

Predstavljena raziskava opisuje uÄinke olja Ärne kumine (Nigella sativa) na aktivnost jetrnih encimov, telesno težo in hepatosomatski indeks pri Å¡vicarskih albino miÅ¡ih pri akutni zastrupitvi, povzroÄeni z natrijevim diklofenakom in ibuprofenom. Za oceno Å¡kodljivega uÄinka diklofenaka in ibuprofena na jetra ter zaÅ¡Äitnega uÄinka olja N. sativa na jetra je bilo testiranih Å¡estintrideset zdravih odraslih miÅ¡i. Živali so bile razdeljene v kontrolno skupino in v pet poskusnih skupin. Živali v kontrolni skupini so prejele fizioloÅ¡ko raztopino (0,9 % NaCl), medtem ko so dobile živali v poskusnih skupinah razliÄne subletalne odmerke diklofenaka in ibuprofena samostojno ali pa v kombinaciji z oljem Ärne kumine. Rezultati so pokazali statistiÄno znaÄilno (p < 0,05) znižanje telesne mase pri skupinah miÅ¡k, ki so dobivale diklofenak in ibuprofen. Pri vseh skupinah, tretiranih z diklofenakom in iboprufenom, so se pokazale statistiÄno znaÄilne razlike med kontrolno in tretiranimi skupinami, kar potrjuje hepatotoksiÄni vpliv tretiranja.  Hepatosomatski indeks je pokazal statistiÄno znaÄilne (p < 0,05) razlike med skupinami, ki so bile tretirane s kombinacijo uÄinkovin, in skupinami, ki so prejemale tudi olje Ärne kumine, kar kaže na hepatoprotektivni uÄinek olja Ärne kumine.

KljuÄne besede: diklofenak; ibuprofen; hepatotoksiÄnost; hepatoprotektivnost; olje N. sativa


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References


(1) Agúndez JA, Lucena MI, Martínez C, et al. Assessment of non-steroidal anti-inflammatory drug-induced hepatotoxicity. Expert Opin Drug Metab Toxicol 2011; 7: 817–28.

(2) Mahalakshmi R, Rajesh P, Ramesh N, Balasubramanian V, Kannan VR. Hepatoprotective activity on Vitexnegundo Linn. (Verbenaceae) by using wistar albino rats in ibuprofen induced model. Int J Pharmacol 2010; 6: 658–63.

(3) Tarasankar M, Ahmad A, Pahari N, Ganguli S. Hepatoprotective activity of Mikania scandes (L.) wild against diclofenac sodium induced liver toxicity in rats. Asian J Pharm Sci 2012; 5: 185-9.

(4) Sadasivan S, Latha PG, Sasikumar JM, Rajashekaran S, Shayamal S, Shine VJ. Hepatoprotective studies on Hedyotiscorymbosa (L) Lam. J Ethnopharmacol 2006; 106: 245–9.

(5) Al-Johar D, Shinwari N, Arif J. Role of Nigella sativa and a number of its antioxidant constituents towards azoxymethane-induced genotoxic effects and colon cancer in rats. J Phytother Res 2008; 22: 1311–23.

(6) Al-Ali A, Al-Khawajah AA, Randhawa MA, Shaikh NA. Oral and intraperitoneal LD50 of thymoquinone, an active principle of Nigella sativa, in mice and rats. J Ayub Med Coll Abbottabad 2008; 20: 25–7.

(7) Ait Mbarek L, Ait Mouse H, Elabbadi N, et al. Anti-tumor properties of black seed (Nigella sativa L.) extracts. Braz J Med Biol Res 2007; 40: 839–47.

(8) Danladi J, Abdusalam A, Timbuak JA, Miriga AA, Dahiru AU. Hepatoprotective effect of black seed (Nigella sativa) oil on carbon tetrachloride (CCL4) induced liver toxicity in adult wistar rats. J Dental Med Sci 2013; 4: 56–62.

(9) Khan MA, Chen HC, Tania M, Zhang DZ. Anticancer activities of Nigella sativa (black cumin). Afr J Tradit Complement Altern Med 2011; 8: 226–32.

(10) Majdalawieh, AF, Fayyad, MW. Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review. Int Immnuno Pharmacol 2015; 28: 295–304.

(11) Mohamed HA, El-Sayed IH, Moawad M. Protective effect of Nigella sativa seeds against dimethylaminoazobenzene (DAB) induced liver carcinogenesis. J Med Biol 2010; 8: 80–7.

(12) El-Gharieb MA, El-Masry TA, Emara AM, Hashem MA. Potential hepatoprotective effects of vitamin E and Nigella sativa oil on hepatotoxicity induced by chronic exposure to malathion in human and male Albino rats. Toxicol Environ Chem 2010; 92: 39–40.

(13) Basavraj ST, Fefar DT, Prajapati KS, et al. Haemato-biochemical alterations induced by diclofenac sodium toxicity in Swiss albino mice. Vet World 2012; 5: 417–9.

(14) Zayed MF, Hassan MH. Synthesis and biological evaluation studies of novel quinazolinone derivatives as antibacterial and anti-inflammatory agents. Saudi Pharm J 2014; 22: 157–62.

(15) Seval D, Evran B, Kalaz EB, Erata GO. Protective effect of Nigella sativa oil against binge ethanol- induced oxidative stress and liver injury in rats. Chin J Nat Med 2014; 12: 495–9.

(16) Hussain I, Khan MZ, Khan A, Javed I, Saleemi K. Toxicological effects of diclofenac in four avian species. Avian Pharm 2009; 37: 315–21.

(17) Al-Khafaji NM. Protective effect of crude oil of Nigella sativa on liver in male albino mice treated with low toxic dose of paracetamol. J Med Chem 2013; 10: 930–6.

(18) Basil AA, Bambosa AO, Al-Hawsawi Z A. Effect of Nigella sativa on blood lipids in normal rats. Arab Gulf J Sci Res 2003; 21: 102–9.

(19) Ilhan N, Seçkin D. Protective effect of Nigella sativa seeds on CCl4 induced hepatotoxicity. FÜ Sağlık Bil Dergisi 2005; 19: 175–9.

(20) Waggan IA, Siddiqui N, Khan N, Jokhio AL. Effect of the diclofenac sodium (NSAID) in caecal mucosa of albino rats. J Pak Pharm 2009; 26: 7-11.

(21) Dollah MA, Parhizkar S, Latiff LA, Hassan MHB. Toxicity effect of Nigella sativa on the liver function of rats. Adv Pharm Bulletin 2013; 3: 97–102.

(22) Khalife KH, Lupidi G. Non-enzymatic reduction of thymoquinone in physiological conditions. Free Radic Res 2007; 41: 153–61.

(23) Guiloski IC, Ribas CLJ, Pereira SL, Neves APP, Silva CH. Effects of trophic exposure to dexamethasone and diclofenac in freshwater fish. Ecotoxicol Environ 2015; 114: 204–11.

(24) Baravalia Y, Vaghasiya YN Chanda S. Hepatoprotective effect of woodfordia fruticosa Kurz flowers on diclofenac sodium induced liver toxicity in rats. J Asian Tropic Med 2011; 4: 342–6.

(25) Tan RJ, Chakravarthi S, Judson PJ, Haleagrahara N, Segarra I. Potential protective effect of sunitinib after administration of diclofenac: biochemical and histopathological drug interaction assessment in a mouse model. Naunyn-Schmiedeberg's Arch Pharmacol 2013; 386: 619–33.

(26) Thapa BR, Anuj W. Liver function tests and their interpretation. Indian J Pediatr 2007; 74: 663–71.

(27) Thangathirupathi A, Saraswathi A, Murugesh N, Ali NA. Hepatoprotective activity of various extracts of Cayratia carnosa (Wall. Ex. Weight) Gagnep. In paracetamol induced hepatotoxicity in Albino rats. Int J Pharm Pharm Sci 2013; 5: 957–60.

(28) Quinn B, Schmidt W, Rourke K, Hernan R. Effects of the pharmaceuticals gemfibrozil and diclofenac on biomarker expression in the zebra mussel (Dreissena polymorpha) and their comparison with standardized toxicity tests. Chemosphere 2011; 84: 657–63.

(29) Ramzan M, Ashraf M, Hashmi HA, Iqbal Z, Anjum AA. Evaluation of diclofenac sodium toxicity at different concentrations in relation to time using broiler chicken model. J Toxicol Sci 2015; 25: 357–65.

(30) Singh A, Bhat TK, Sharma OP. Clinical biochemistry of hepatotoxicity. J Clin Toxicol 2011; 4: 1–19.

(31) Aprioku JS, Nwidu LL, Amadi CN. Evaluation of toxicological profile of ibuprofen in wistar albino rats. Am J Bio Med Sci 2014; 6: 32–40.

(32) Gani MS, John SA. Evaluation of hepatoprotective effect of Nigella sativa L. Int J Pharm Sci 2013; 5: 428–30.


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