THE LITTLE WHITE EGRET (Egretta garzetta) AS A POTENTIAL SOURCE OF MUL-TIDRUG-RESISTANT AVIAN PATHOGENIC E. coli
Abstract: Avian pathogenic Escherichia coli (APEC) is a significant threat that affect domesticated and wild birds. Studies related to the prevalence of APEC in the migratory and wild birds are relatively few compared with those related to other avian species. In particular, the role of the little white egret (Egretta garzetta) as a carrier and a reservoir for transmission of the APEC to other avian species had been neglected. Therefore, this work was done to study the occurrence of multidrug-resistant (MDR) APEC in the extraintestinal tissues of the little white egret. The overall isolation percentage of APEC was 20%. The highest isolation percentage was recorded in lungs followed by air sacs, heart blood, liver, and kidneys with percentages of 20%, 15%, 10%, 10%, and 5%, respectively. Sereotyping of E. coli isolates revealed, 4 (33.33%) strains of O26:K6, 3 (25%) strains of O78:K80, 2 (16.66%) strains of O114:K90, 2 (16.66%) strains of O2:K1, and 1(8.33%) strain of O127:K63. Virulence-associated genes including arginine succinyltransferase (astA), temperature sensitive hemagglutinin (tsh), putative avian hemolysin (hlyF), and iron outer membrane receptor (iroN) were screened using polymerase chain reaction (PCR) and all of tested genes were detected in E. coli serotype O78:K80. All E. coli isolates showed drug resistance to at least one of the 12 antimicrobials tested, with remarkable high resistance (100%) to ampicillin, nalidixic acid, and penicillin. In conclusion, the little white egret should be considered as a potential carrier for MDR APEC.
Key words: E. coli; little white egret; multidrug resistance; virulence attributes
~ 1. Ammar AM, Abd El-Aziz NK, Abd El Wanis S, et al. Molecular versus conventional culture for detection of respiratory bacterial pathogens in poultry Cell Mol Biol (2016): 62: 52-56.
~ 2. Abd El-Aziz NK, Gharib AA, Mohamed EAA, et al. Real-time PCR versus MALDI-TOF MS and culture-based techniques for diagnosis of bloodstream and pyogenic infections in humans and animals. J Appl Microbiol (2020); 130: 1630-1644.
~ 3. Subedi M, Bhattarai RK, Devkota B, et al. Correction to: Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal. BMC Vet Res 2018; 14:166.
~ 4. Xi Y, Wood C, Lu B, et al. Prevalence of a septicemia disease in the crested ibis (Nipponia nippon) in China. Avian Dis 2007; 51:614–7.
~ 5. Oaks JL, Besser TE, Walk ST, et al. Escherichia albertii in wild and domestic birds. Emerg Infect Dis 2010; 16:638–46.
~ 6. Darwish WS, Eldaly E, El-Abbasy M, et al. Antibiotic residues in food: African scenario. Jap J Vet Res 2013; 61:S13–S22.
~ 7. O'Brien TF. Emergence, spread, and environmental effect of antimicrobial resistance: how use of an antimicrobial anywhere can increase resistance to any antimicrobial anywhere else. Clin Infect Dis 2002; 34:S78–84.
~ 8. Elmowalid GA, Ahmad AAM, Hassan MN, et al. Molecular detection of new SHV β-lactamase variants in clinical Escherichia coli and Klebsiella pneumoniae isolates from Egypt. Comp Immunol Microbiol Infect Dis (2018); 60: 35-41
~ 9. Ashbolt NJ, Amézquita A, Backhaus T, et al. Human Health Risk Assessment (HHRA) for environmental development and transfer of antibiotic resistance. Environ Health Perspect 2013; 121:993–1001.
~ 10. Abd El-Aziz NK and Gharib AA. Coexistence of plasmid-mediated quinolone resistance determinants and AmpC-BetaLactamases in Escherichia coli strains in Egypt. Cell Mol Biol (2015); 61: 29-35
~ 11. Ahlstrom CA, Bonnedahl J, Woksepp H, et al. Acquisition and dissemination of cephalosporin-resistant E. coli in migratory birds sampled at an Alaska landfill as inferred through genomic analysis. Sci Rep 2018; 8:7361.
~ 12. Mohsin M, Raza S, Schaufler K, et al. High prevalence of CTX-M-15-Type ESBL-Producing E. coli from migratory avian species in Pakistan. Front Microbiol 2017; 8:2476.
~ 13. Najdenski H, Dimova T, Zaharieva MM, et al. Migratory birds along the Mediterranean - Black Sea Flyway as carriers of zoonotic pathogens. Can J Microbiol 2018; 64:915–924.
~ 14. Chen C, Cui CY, Zhang, Y, et al. Emergence of mobile tigecycline resistance mechanism in Escherichia coli strains from migratory birds in China. Emerg microbes Infect 2019; 8:1219–1222.
~ 15. Fahim KM, Ismael E, Khalefa HS, et al. Isolation and characterization of E. coli strains causing intramammary infections from dairy animals and wild birds. Int J Vet Sci Med 2019; 7:61–70.
~ 16. Cloud SS, Rosenberger JK, Fries PA, et al. In vitro and in vivo characterization of avian Escherichia coli. I. Serotypes, metabolic activity, and antibiotic sensitivity. Avian Dis 1985; 29:1084–1093.
~ 17. Kok T, Worswich D, Gowans E. Some serological techniques for microbial and viral infections. In Practical Medical Microbiology (Collee, J.; Fraser, A.; Marmion, B. and Simmons, A., eds.), 14th ed., Edinburgh, Churchill Livingstone, UK. 1996.
~ 18. Boom R, Sol C, Salimans M, et al. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 28:495–503.
~ 19. Wayne P. Performance standards for antimicrobial susceptibility testing. CLSI approved standard M100–S23. Clin Lab Stand Inst 2013; 33:118–156.
~ 20. Kabir S. Avian colibacillosis and salmonellosis: a closer look at epidemiology, pathogenesis, diagnosis, control and public health concerns. Int J Environ Res Public Health 2010; 7:89–114.
~ 21. Darwish WS, Saad Eldin WF, Eldesoky KI. Prevalence, molecular characterization and antibiotic susceptibility of Escherichia coli isolated from duck meat and giblets. J Food Safety 2015; 35:410–415.
~ 22. De Carli S, Ikuta N, Lehmann FK, et al. Virulence gene content in Escherichia coli isolates from poultry flocks with clinical signs of colibacillosis in Brazil. Poult Sci 2015: 94:2635–40.
~ 23. SaadEldin WF. Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese quails reared in Sharkia Governorate, Egypt. Damanhour J Vet Sci 2019; 1:14–17.
~ 24. Nguyen RN, Taylor LS, Tauschek M, et al. Atypical enteropathogenicEscherichia coli infection and prolonged diarrhea in children. Emerg Infect Dis 2006; 12:597–603.
~ 25. Fadel HM, Afifi R, Al-Qabili DM. Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species. Vet World 2017;10:1118-1128.
~ 26. Callaway TR, Edrington TS, Nisbet DJ. Isolation of Escherichia coli O157:H7 and Salmonella from migratory brown-headed cowbirds (Molothrus ater), common Grackles (Quiscalus quiscula), and cattle egrets (Bubulcus ibis). Foodborne Pathog Dis 2014;11:791-4..
~ 27. Alizade H, Ghanbarpour R, Jajarami M, et al. Phylogenetic typing and molecular detection of virulence factors of avian pathogenic Escherichia coli isolated from colibacillosis cases in Japanese quail. Vet Res Forum 2017; 8:55–58.
~ 28. Dozois CM, Dho-Moulin M, Brée A, et al.. Relationship between the Tsh autotransporter and pathogenicity of avian Escherichia coli and localization and analysis of the Tsh genetic region. Infect Immun 2000; 68:4145–54.
~ 29. Bailey MJ, Koronakis V, Schmoll T, et al. Escherichia coli HlyT protein, a transcriptional activator of haemolysin synthesis and secretion, is encoded by the rfaH (sfrB) locus required for expression of sex factor and lipopolysaccharide genes. Mol Microbiol 1992; 6:1003–12.
~ 30. Schneider BL, Kiupakis AK, Reitzer LJ. Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli. J Bacteriol 1998; 180:4278–86.
~ 31. Magistro G, Hoffmann C, Schubert S. The salmochelin receptor IroN itself, but not salmochelin-mediated iron uptake promotes biofilm formation in extraintestinal pathogenic Escherichia coli (ExPEC). Int J Med Microbiol 2015; 305:435–45.
~ 32. Na SH, Moon DC, Choi MJ, et al. Antimicrobial resistance and molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from ducks in South Korea. Foodborne Pathog Dis 2019; 16:799-806.
~ 33. Ramey AM, Hernandez J, Tyrlöv V, et al. Antibiotic-resistant Escherichia coli in migratory birds inhabiting remote Alaska. Ecohealth 2018; 15:72–81.
~ 34. Ahmed ZS, Elshafiee EA, Khalefa HS, et al. Evidence of colistin resistance genes (mcr-1 and mcr-2) in wild birds and its public health implication in Egypt. Antimicrob Resist Infect Control 2019; 8:197.
~ 35. Wu J, Huang Y, Rao D, et al. Evidence for environmental dissemination of antibiotic resistance mediated by wild birds. Front Microbiol 2018; 9:745.