EFFECTS OF AGE AND SEASON ON SEMEN CHARACTERISTICS IN ARABIAN STALLIONS UNDER SUBTROPICAL CONDITIONS OF KUWAIT
DOI:
https://doi.org/10.26873/SVR-1450-2021Keywords:
Kuwait, Arabian stallions, age, seasons, semen charactersAbstract
Semen analysis is a valuable approach to assess stallion fertility. Many factors, including the age of the stallion and the season of year have been shown to influence semen quality. Therefore, the current study aimed to evaluate the influence of stallion’s age and season on the potential semen parameters and sperm morphology in Arabian stallions. A total of 108 ejaculates were collected using artificial vagina throughout the year from nine Arabian stallions aged 4-22 years, divided into three groups according to their ages; Group I (4-6 years), Group II (8-11years), and Group III (15-22 years). The ejaculates were analyzed immediately after collection for macroscopic and microscopic parameters using computer-assisted sperm analysis (CASA). Statistical analysis was applied to study the effect of the age of the stallion and season of semen collection on semen parameters. Our results showed a clear age-based differences (P < 0.05) with stallions aged from 4 to 11 years produce higher semen quality especially in sperm count, livability, motility and normal sperms compared with old stallions (15-22 years). Moreover, old stallions showed higher (P < 0.05) sperm abnormalities especially the secondary defects. The semen volume increased (P < 0.05) in the spring, and sperm concentration was higher (P < 0.05) in the winter and autumn, while, no significant variation in total sperm number between different seasons. Moreover, the livability, motility, normal sperms and progressive sperm motility were higher (P < 0.05) in hot season (breeding season) compared with cold season, but abnormal sperms were lower. These data suggest that, older stallions (15-22 years) produce ejaculates of larger volume but the motility, viability, and abnormalities percent were better in ejaculates collected from stallions aged 4-11 years. Ejaculates collected during spring and summer showed better characteristic than that collected during autumn and winter.
References
● 1. Whitesell K, Stefanovski D, McDonnell S, et al. Evaluation of the effect of laboratory methods on semen analysis and breeding soundness examination (BSE) classification in stallions. Theriogenology 2020; 142: 67–76.
● 2. Kowalczyk A, Czerniawska-Piatkowska E, Kuczaj M. Factors influencing the popularity of artificial inse-mination of mares in Europe. Animals (Basel) 2019; 9.
● 3. Vilakazi D, Webb E. Effect of age and season on sperm morphology of Friesland bulls at an artificial insemination centre in South Africa. S Afr J Anim Sci 2004; 34: 62–9.
● 4. Pickett BW, Faulkner LC, Voss JL. Effect of season on some characteristics of stallion semen. J Reprod Fertil Suppl 1975; 25–8.
● 5. Allen WR, Wilsher S. The influence of mare numbers, ejaculation frequency and month on the fertility of Thoroughbred stallions. Equine Vet J 2012; 44: 535–41.
● 6. Dowsett K, Pattie W. Variation in characteristics of stallion semen caused by breed, age, season of year and service frequency. J Reprod Fertil Suppl 1987; 35: 645–7.
● 7. Vasan SS. Semen analysis and sperm function tests: How much to test? Indian J Urol 2011; 27: 41–8.
● 8. Brito LFC. Evaluation of stallion sperm morphology. Clin Tech Equine Pract 2007; 6: 249–64.
● 9. Love CC, Varner DD, Thompson JA. Intra- and inter-stallion variation in sperm morphology and their relationship with fertility. J Reprod Fertil Suppl 2000; 93–100.
● 10. Köhne M, Brüning P, Stuhtmann G, et al. Semen quality evaluation in young stallions–feasibility and comparison of two different protocols. Pferdeheilkunde Equine Medicine 2020; 36: 11–9.
● 11. Colenbrander B, Feitsma H, Grooten HJ. Optimizing semen production for artificial insemination in swine. J Reprod Fertil Suppl 1993; 48: 207–15.
● 12. Auger J, Jouannet P, Eustache F. Another look at human sperm morphology. Hum Reprod 2016; 31: 10–23.
● 13. Jasko DJ, Lein DH, Foote RH. Determination of the relationship between sperm morphologic classifications and fertility in stallions: 66 cases (1987-1988). J Am Vet Med Assoc 1990; 197: 389–94.
● 14. Voss JL, Pickett BW, Squires EL. Stallion spermatozoal morphology and motility and their relationships to fertility. J Am Vet Med Assoc 1981; 178: 287–9.
● 15. Dowsett KF, Pattie WA. Characteristics and fertility of stallion semen. J Reprod Fertil Suppl 1982; 32: 1–8.
● 16. Kumaresan A, Das Gupta M, Datta TK, et al. Sperm DNA integrity and male fertility in farm animals: a review. Front Vet Sci 2020; 7: 321.
● 17. Menon AG, Barkema HW, Wilde R, et al. Associations between sperm abnormalities, breed, age, and scrotal circumference in beef bulls. Can J Vet Res 2011; 75: 241–7.
● 18. Blom E. Pathological conditions in genital organs and sperm as a cause for the rejection of breeding bulls for import into and export from Denmark (an andrologic retrospective, 1958-1982). Nord Vet Med 1983; 35: 105–30.
● 19. Hallap T, Jaakma U, Rodriguez-Martinez H. Changes in semen quality in Estonian Holstein AI bulls at 3, 5 and 7 years of age. Reprod Domest Anim 2006; 41: 214–8.
● 20. Brito LF, Silva AE, Rodrigues LH, et al. Effects of environmental factors, age and genotype on sperm produ-ction and semen quality in Bos indicus and Bos taurus AI bulls in Brazil. Anim Reprod Sci 2002; 70: 181-90.
● 21. Dowsett KF, Knott LM. The influence of age and breed on stallion semen. Theriogenology 1996; 46: 397–412.
● 22. Mathevon M, Buhr MM, Dekkers JC. Environmental, management, and genetic factors affecting semen production in Holstein bulls. J Dairy Sci 1998; 81: 3321–30.
● 23. D’Andre HC, Rugira KD, Elyse A, et al. Influence of breed, season and age on quality bovine semen used for artificial insemination. Int J Livest Prod 2017; 8: 72–8.
● 24. Almquist JO. Effect of long term ejaculation at high frequency on output of sperm, sexual behavior, and fertility of Holstein bulls; relation of reproductive capacity to high nutrient allowance. J Dairy Sci 1982; 65: 814–23.
● 25. Love CC. Measurement of concentration and vi-ability in stallion sperm. J Equine Vet Sci 2012; 32: 464-6.
● 26. Sieme H, Katila T, Klug E. Effect of semen collection practices on sperm characteristics before and after storage and on fertility of stallions. Theriogenology 2004; 61: 769–84.
● 27. Hanulakova S, Mamica O, Máchal L, et al. Physical properties of stallion semen in relation to some qualitative and quantitative characteristics. Acta Univ Agric Silvic Mendel Brun 2012; 12: 97–101.
● 28. Najjar A, Benaoun B, Ezzaouia M, et al. De-termination of semen and sexual behavior parameters of Arabian stallions to be selected for an artificial insemination program under Tunisian conditions. Am Eur J Agric Environ Sci 2010; 8: 173–7.
● 29. Pickett B, Faulkner L, Seidel Jr G, et al. Reproductive physiology of the stallion. IV. Seminal and behavioral characteristics. J Anim Sci 1976; 43: 617–25.
● 30. Dowsett K. The effect of service frequency and season of year on stallion semen. Aust Equine Vet 1997; 15: 16–26.
● 31. Amann RP, Waberski D. Computer-assisted sperm analysis (CASA): capabilities and potential developments. Theriogenology 2014; 81: 5–17.
● 32. Waddington B, Penitente-Filho JM, Neves J, et al. Testosterone serum profile, semen characteristics and testicular biometry of Mangalarga Marchador stallions in a tropical environment. Reprod Domest Anim 2017; 52: 335–43.
● 33. Shehata Y, Khater S, El-Bayomi H, et al. Reproprotective effects of moderate exercise in male rats doped with testosterone enanthate. Zagazig Vet J 2020; 48: 189–98.
● 34. Janett F, Thun R, Niederer K, et al. Seasonal changes in semen quality and freezability in the Warmblood stallion. Theriogenology 2003; 60: 453–61.
● 35. Morrell JM, Richter J, Martinsson G, et al. Pregnancy rates after artificial insemination with cooled stallion spermatozoa either with or without single layer centrifugation. Theriogenology 2014; 82: 1102–5.
● 36. Rota A, Furzi C, Panzani D, et al. Studies on motility and fertility of cooled stallion spermatozoa. Reprod Domest Anim 2004; 39: 103–9.
● 37. Nardone A, Ronchi B, Lacetera N, et al. Effects of climate changes on animal production and sustainability of livestock systems. Livest Sci 2010; 130: 57–69.
● 38. Zervos IA, Lavrentiadou SN, Tsantarliotou MP, et al. Seasonal variation of plasminogen activator activity in spermatozoa and seminal plasma of boar, buck, bull and stallion. Reprod Domest Anim 2010; 45: e440–6.
● 39. Zucker I, Johnston P, Frost D. Comparative, physiological and biochronometric analyses of rodent seasonal reproductive cycles. Prog Reprod Biol 1980; 5: 102–33.
● 40. Leme DP, Papa FO, Roser JF. Reproductive characteristics of stallions during the breeding and non-breeding season in a tropical region. Trop Anim Health Prod 2012; 44: 1703–7.
● 41. Kandiel MMM, El Khawagah ARM. Evaluation of semen characteristics, oxidative stress, and biochemical indices in Arabian horses of different ages during the hot summer season. Iran J Vet Res 2018; 19: 270–5.
● 42. Clay CM, Squires EL, Amann RP, et al. Influences of season and artificial photoperiod on stallions: testicular size, seminal characteristics and sexual behavior. J Anim Sci 1987; 64: 517–25.
● 43. Magistrini M, Chanteloube P, Palmer E. Influence of season and frequency of ejaculation on production of stallion semen for freezing. J Reprod Fertil Suppl 1987; 35: 127–33.
● 44. Erb R, Andrews F, Hilton J. Seasonal variation in semen quality of the dairy bull. J Dairy Sci 1942; 25: 815–26.
● 45. Valeanu S, Johannisson A, Lundeheim N, et al. Seasonal variation in sperm quality parameters in Swedish red dairy bulls used for artificial insemination. Livest Sci 2015; 173: 111–8.
● 46. Turner RM. Current techniques for evaluation of stallion fertility. Clin Tech Equine Pract 2005; 4: 257–68.
● 47. Suliman Y, Becker F, Tuchscherer A, et al. Seasonal variations in quantitative and qualitative sperm characteristics in fertile and subfertile stallions. Arch Anim Breed 2020; 63: 145–54.
● 48. Gamboa S, Francisco M, Gomes P, et al. Level of glycolyzable substrates in stallion semen: effect of ejaculation frequency on sperm survival after cool storage during the nonbreeding season. J Equine Vet Sci 2011; 31: 109–15.
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Copyright (c) 2021 Gamal Shawki, Tawfek Mohamed Barakat, Attia Mohamed Samy, Ahmed Alhamed AL-Mejren, Ayman Mesalam
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