ISOLATION AND CHARACTERIZATION OF FETAL AND NEONATAL LIVER-DERIVED MESENCHYMAL STEM CELLS FROM RABBITS

Authors

  • Esraa Elsheikh Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Zagazig University. Alzeraa Street, Postal code 44511, Zagazig City, Al-Sharkia Province, Egypt
  • Enas EL-Hady 1Anatomy and Embryology Department, Faculty of Veterinary Medicine, Zagazig University
  • Somia Abdallah 2 Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University
  • Atef Selem 1Anatomy and Embryology Department, Faculty of Veterinary Medicine, Zagazig University
  • Mervat Konsowa 1Anatomy and Embryology Department, Faculty of Veterinary Medicine, Zagazig University

Abstract

Abstract: Mesenchymal stem cells (MSCs) became in recent years a promising implement for cell therapies. Our study described the isolation and characterization of liver mesenchymal stem cells (LMSCs). We isolated LMSCs from the rabbit liver tissue from two groups: 20th day's old fetuses and 4 days-old kittens. LMSCs were characterized by flow cytometry, immunohistochemistry (IHC), and quantitative real-time-polymerase chain reaction (qRT-PCR). Flow cytometry of the isolated LMSCs showed variable expression of the cluster of differentiation markers (CD) including, CD105, CD106, CD11b, CD38, CD63, CD9, and CD14. Fetal liver isolated LMSCs showed expression of CD105, CD106, CD9, and CD63 but no expression of CD14, CD11b, and CD38 was observed. Absolute expression of LMSCs was performed by qRT-PCR analysis to detect cytoskeleton genes including, vimentin, nestin, and desmin in the experimental groups. In vivo localization of stem cell markers such as CD105 (Endoglin), vimentin, and desmin was also distinguished in fetal and neonatal liver tissues using IHC.

 

Keywords: Liver; Mesenchymal stem cells; Immunohistochemistry; Flow cytometry; Rabbit.

 

 

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Published

2021-12-17

How to Cite

Elsheikh, E., EL-Hady, E., Abdallah, S., Selem, A., & Konsowa, M. (2021). ISOLATION AND CHARACTERIZATION OF FETAL AND NEONATAL LIVER-DERIVED MESENCHYMAL STEM CELLS FROM RABBITS. SLOVENIAN VETERINARY RESEARCH, 58(24-Suppl). Retrieved from https://slovetres.si/index.php/SVR/article/view/1438

Issue

Vol 58, Suppl 24 (2021)

Section

Original Research Article