THE IMMUNOSTIMULANT EFFECT OF SACCHAROMYCES CEREVISIAE AND THE IMPACT OF FUSARIUM SOLANI INFECTION ON OREOCHROMIS NILOTICUS
DOI:
https://doi.org/10.26873/SVR-1426-2021Abstract
Abstract:This study summarizes the impact of Fusarium solani on Oreochromis niloticus with evaluation of the efficacy of Saccharomyces cerevisiae for decreasing its possible consequences on fish health was investigated. Fifty samples of fish ration were collected for isolation and identification of Fusarium solani. One hundred and sixty O. niloticus clinically healthy, average weight of 59.25±1.04 g was distributed into four groups. First group was a control negative group (C). Second group (F) was infected with Fusarium solani (1x 104 zoospores of Fusarium /ml). Third group (F+S) was fed a basal diet containing S. cervisaie 1 g/ kg ration daily and injected intraperitoneally with Fusarium solani. Fourth group (S) was fed a basal diet containing (S. cervisaie) daily. The experiment extended for five weeks then all groups were challenged with Pseudomonas aeroginosa (3x 107 CFU/ml). Bacteriological findings of the intestinal microbiota revealed significant increase (log10 CFU/ml) in total bacterial count (TBC) in the (F+S) group (4.51±0.012) and decreased gradually from the 1st week to the 5th week, while the (S) group showed slight increase in TBC than (C) group (P<0.05). The haemato-immunological parameters after 3 weeks in the (S) group showed significant increase in serum lysozyme activity and total leukocytic count. After five weeks there were a marked decrease in serum lysozyme activity and total leukocytic count in (F) group. After the challenge, there was a significant decrease in serum lysozyme activity and total leukocytic count in (F) group. Lymphocyte count and gamma globulin were significantly higher in (S) group than group (C) (P<0.05). The Pathological lesions in gills, liver, muscles in (F) group showed sever degenerative changes, necrosis and leucocytic infiltrations. The lesions in group (F+S) were milder. It could concluded that S. cerevisiae can be used in ration of O. niloticus under immune-depressive stressful condition (fungal infection) to increase their resistance.
Key words: Fusarium solani, Oreochromis niloticus, Saccharomyces cerevisiae, immunostimulants.
References
● 1. Ali EH, Hashem M, Al-Salahy MB. Pathogenicity and oxidative stress in Nile tilapia caused by Aphanomyces laevis and Phoma herbarium isolated from farm fish. Dis Aquat Organ 2011; 94:17–28.
● 2. Bondy GS, Pestka JJ. Immunomodulation by fungal toxins. J Toxicol Env Heal. B 2000; 3:109–43.
● 3. Abd El-Ghany NA, El-Khatib NR, Salama SS. Causes of mortality in Discus fish (Symphysodon) and trials for treatment. Egy J aquac 2014; 4:1–12.
● 4. D’mello J, Placinta C, Macdonald A. Fusarium mycotoxins: A review of global implications for animal health, welfare and productivity. Ani Feed Sc Tech 1999; 80: 183–205.
● 5. Esteban MÁ, Cerezuela R. 4 - Fish mucosal immunity: skin. In Mucosal Health in Aquaculture, Beck, B.H., Peatman, E., Eds. Academic Press: San Diego, 2015; 67–92.
● 6. Abd El Aziz MA, Kamel GH, Mahamoud MA. Seasonal study histopahlogical and treatment trial on Saprolegniosis in some fish farms. Irst Ann Confr, FVM Moshtohor. Scientific conference of veterinary medicine. Uni Banha Moshtohor. 2004; 1-4.
● 7. Cabral João PS. Water Microbiology. Bacterial Pathogens and Water. Int J Environ Res Public Health 2010; 7: 3657–703.
● 8. Olabuenaga SE. Fish immune system immune. Gayana (Concepción). Scielo 2000; 64: 205–15.
● 9. Magnadóttir B. Immunological control of fish diseases. Mar. Biotechnol 2010; 12: 361–79.
● 10. Lieschke GJ, Trede NS. Fish immunology. Curr Biol 2009; 19: R678-R682.
● 11. Acton RT, Weinheimer PF, Dupree HK, et al. Isolation and characterization of the immune macroglobulin from the paddle fish, Polyodon spathula. J Biol Chem 1971; 246: 6760–9.
● 12. Hoseinifar SH, Dadar M, Ringø E. Modulation of nutrient digestibility and digestive enzyme activities in aquatic animals: The functional feed additives scenario. Aquac Res 2017; 48: 3987–4000.
● 13. Carballo C, Pinto PIS, Mateus AP, et al. Yeast β‐glucans and microalgal extracts modulate the immune response and gut microbiome in Senegalese sole (Solea senegalensis). Fish Shellfish Immunol 2019; 92: 31–9.
● 14. Rodríguez A, Cuesta A, Ortuño J, et al. Immunostimulant properties of a cell wall‐modified whole Saccharomyces cerevisiae strain administered by diet to seabream (Sparus aurata L.). Veterinary Immunology and Immunopathology 2003; 96: 183–92.
● 15. Whitman KA. Finfish and Shellfish Bacteriology Manual; Techniques and Procedures. Iowa State Press. 2004.
● 16. Burgess LW. General ecology of the Fusaria. In: Nelson PE, Toussoun TA, Cook RJ, editors. Fusarium: Disease, Biology and Taxonomy. Pennsylvania State University Press; University Park. 1981; 225–35.
● 17. Hassan NM, Selim AS. Pathogenic potential of some fungal species of the genera Aspergillus, Mucor and Rhizopus. Arch Exper Vet Med Leipzig 1984; 38: 687–91.
● 18. Mohamed HMA, Emeish WFA, Braeuning A, Hammad S. Detection of aflatoxin producing fungi isolated from Nile tilapia and fish feed. EXCLI J 2017; 16: 1308––18.
● 19. Plumb JA, Bowser PR. Microbial fish diseases laboratory manual. Auburn University Alabama Agricultural Experiment station, Auburn, Alabama. 1983.
● 20. Cruickshank R, Duguid JP, Marion BP, et al. Medical Microbiology. 12th Edit, Vol. II. Churchill Livingstone, Edinburgh, London and New York. 1975.
● 21. Stokpof M. Fish Medicine, WB Saundres Company. Philadelphia, London, Toronto, Montreal, Sydney, Toyo 1993; 113-131& 149–59.
● 22. Schltz LA. Veterinary Hematology. Third Edit. Lea and Febiger 1987; 39:217–22.
● 23. Sonnenwirth AC, Jarrett L. Gradwohl's. Clinical laboratory methods and diagnosis. Mosby, New York, 1980.
● 24. Davis B. Disk electrophoresis. Method and application to human serum protein. Ann NY Acad Sc 1964; 121:404-–27.
● 25. Bancroft J, Gamble M. Theory and Practice of Histological Techniques. 6th Edition, Churchill Livingstone, Elsevier, China, 2008.
● 26. Ruangroupan L, Kitao T, Yoshida T. Protective efficacy of Aeromonas hydrophila vaccines in Nile tilapia. Vet. Immunol Immunopathol 1986; 12: 345–50.
● 27. Austin B, Austin DA. Bacterial Fish Pathogens: Diseases in Causes of mortality in Discus fish (Symphysodon) and trials for treatment. Sixth Ed 2007; 354–56.
● 28. IBM corp. IBM SPSS statistics for windows. Armonk, NY: IBM corp. 2017.
● 29. Palmero D, Iglesias C, de Cara M. et al. Species of Fusarium isolated from river and sea water of southeastern Spain and pathogenicity on four plant species. Plant Dis 2009; 93: 377–85.
● 30. El-Sharouny HM, Badram RA. Experimental transmission and pathogenicity of some zoosporic fungi to Tilapia fish. Mycopathlogia 1995; 132:95–103.
● 31. Setty PH, Jespersen L. Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontaminating agents. Trends Food Sci Technol 2006; 17:48–55.
● 32. de Almada CN, De Almada CN, Martinez RC, et al. Characterization of the intestinal microbiota and its interaction with probiotics and health impacts. Appl Microbiol Biotechnol 2015; 99: 4175–99.
● 33. Abd El-Ghany NA, Abou El-Azab MF, Barakat M, et al. Some studies of some yeast (Saccharomyces cervisiae) to reduce aflatoxins B 1 in Oreochromis niloticus. Abbassa Int J Aqua 2014; 7:1-24.
● 34. Ringø E, Olsen RE, Gonzalez Vecino LJ, et al. Use of immunostimulants and nucleotides in aquaculture: a review. J Marine Sci Res Develop 2012; 2:1–22.
● 35. El-Boshy ME, El-Ashram AM, Abdelhamid FM, et al. Immunomodulatory effect of dietary Saccharomyces cerevisiae, beta-glucan and laminaran in mercuric chloride treated Nile tilapia (Oreochromis niloticus) and experimentally infected with Aeromonas hydrophila. Fish Shellfish Immunol 2010; 28: 802–8.
● 36. Egerton S, Culloty S, Jason Whooley J, et al. The Gut Microbiota of Marine Fish. Front Microbiol 2018; 9:873–85.
● 37. Bagheri T, Hedayati SA, Yavari V, et al. Growth, survival, and gut microbial load of rainbow trout (Onchorhynchus mykiss) fry given diet supplemented with probiotic during the two months of first feeding. Turkish J Fish Aquat Sci 2008; 8: 43–48.
● 38. Abu-Elala N, Marzouk M, Moustafa M. Use of different Saccharomyces cerevisiae biotic form as immune-modulator and growth promotor for Oreochromis nilotocus challenged with some fish pathogens. Int J Vet Sci Med 2013; 1:21–29.
● 39. Hassan O, Hassan A, Abd El Ghany N, et al. Contribution on the pathogenicity of Fusarium oxysporum isolated from cultured Nile tilapia (Oreochromis niloticus) with trials for the treatment. Egyptian Journal of Aquatic Biology 2020; 24: 197–215.
● 40. El-Nobi G, Hassanin M, Khalil AA, et al. Synbiotic Effects of Saccharomyces cerevisiae, Mannan Oligosaccharides, and β-Glucan on Innate Immunity, Antioxidant Status, and Disease Resistance of Nile Tilapia, Oreochromis niloticus. Antibiotics 2021; 10: 567.
● 41. Abbas AK, Lichtman AH, Pillai S. Cellular and molecular immunology (updated 6/E). Saunders Elsevier Philadelphia, PA, USA. 2010.
● 42. Abdlekhalek NK, Zaki VH, Yousef MA. Effects of dietary intake of Saccharomyces cerevisiae on nonspecific immune response and disease resistance of Nile tilapia (Oreochromis niloticus). Conference, Mansoura Uni 2014.
● 43. Khaksefidi A, Ghoorchi T. Effect of probiotics on performance and immunocompetence in broiler chicks. J Poult Sci 2006; 43:296–300.
● 44. Kabir SML, Rahman MM, Ahmed SU. The dynamics of probiotics on growth performance and immune response in broilers. Int J Poult Sci 2004; 3:361–4.
● 45. Jin LZ, Ho YW, Abdullah N, Jalaludin S. Probiotics in poultry: modes of action. World’s Poult Sci J 1997; 53:351–368.
● 46. Chatterjee D, Mukherjee SK, Dey A. Nuclear disintegration in chicken peritoneal macrophages exposed to fumonisin B1 from Indian Maize. Lett Appl Microbiol 1995; 20: 184–95.
● 47. Qureshi MA, Garlich JD, Hagler WM, et al. Fusarium proliferatum culture material alters several production and immune performance parameters in white leghorn chickens. Immunopharmacol Immunotoxicol 1995; 17:791–804.
● 48. Pierron A, Alassane-Kpembi I, Oswald I P. Impact of mycotoxin on immune response and consequences for pig health. Anim Nutr 2016; 2: 63–8.
● 49. Oelschlaeger TA. Mechanisms of probiotic actions–a review. Int J Med Microbiol 2010; 300: 57–62.
● 50. Refai MK, Mohamed LA, Kenawy AM, et al. The assessment of mycotic settlement of freshwater fish in Egypt. Am J Sci 2010; 6: 595-602.
● 51. Espinosa C, Esteban MÁ. Effect of dietary supplementation with yeast Saccharomyces cervisiae on skin, serum and liver of gilthead seabream (Sparus aurata L). J Fish Biol 2020; 97:869–81.
● 52. Yiannikouris A, Andre G, Poughon L, et al. Chemichal and conformational study of the interaction involved mycotoxin complexation with B-glucans. Biomacromolecules 2006; 7:1147–55.
● 53. Ortuno, J.; Cuesta, A.; Rodriguez, A.; Esteban, M.A. and Mesegure, J. Oral administration of yeast, Saccharomyces cerevisiae, enhance the cellular innate immune response of gilthead sea bream (Sparus aurata L). Vet Immunol Immunopathol 2002; 85: 41–50.
● 54. Cutuli MT, Gibello A, Rodriguez-Bertos, A, et al. Skin and subcutaneous mycoses in tilapia (Oreochromis niloticus) caused by Fusarium oxysporum in coinfection with Aeromonas hydrophila. Med Mycol Case Rep 2015; 9: 7–11.
● 55. Steinel NC, Bolnick DI. Melanomacrophage Centers as a Histological Indicator of Immune Function in Fish and Other Poikilotherms. Front Immunol 2017; 8: 827.
● 56. Abu-Aita NA, Yassa VF. Impact of vitamin C on genotoxicity, sperm abnormalities and serum biochemical alterations in deltamethrin exposed rats. Egypt J Comp Patho Clini Patho 2008; 21:1–9.
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Copyright (c) 2021 Nadia Abd El Ghany, Gehan Alagmy, Noha Mohamed Abd elgalil, Neveen Rasheed
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