• Hojjatollah Shokri


  Fungal microbiotas are saprophytic microorganisms that can act as opportunistic pathogens in animals. This study was carried out in order to isolate and identify the ear fungal biota from healthy cattle. The samples were taken using premoistened swabs from the right and/or left external ear canals of 32 healthy cattle and cultured onto Sabouraud glucose agar and modified Dixon's agar media. A total of eight different fungal genera were isolated from 29 (90.6%) of 32 healthy cattle. Both filamentous fungi and yeasts were isolated with the predominance of Aspergillus spp. (35.6%), Candida spp. (18.9%) and Malassezia spp. (16.8%). The most frequent Aspergillus spp. were A. fumigatus (16.8%), A. glaucus (14.9%) and A. flavus (4%). Among the fungal isolates, 46.5% and 17.8% colonies were associated with hyaline and dematiaceous fungi, respectively (p = 0.003). The recognized fungi, especially Aspergillus spp. and Candida spp., were colonizes as mycobiota in the external ear canals of healthy cattle, leading to otitis externa under predisposing factors.


Akerstedt J, Vollset I. Malassezia pachydermatis with special reference to canine skin disease. Br Vet J 1996; 152: 269–81.

Bornand V. Bacteriology and mycology of external otitis in dogs. Schweiz Arch Tierheilkd 1992; 134: 341–8.

Huang HP, Huang HM. Effects of ear type, sex, age, body weight, and climate on temperatures in the external acoustic meatus of dogs. Am J Vet Res 1999; 9: 1173–6.

Nakabayashi A, Sei Y, Guillot J. Identification of Malassezia species isolated from patients with seborrhoeic dermatitis, atopic dermatitis, pityriasis versicolor and normal subjects. Med Mycol 2000; 38: 337–41.

Dukes FAD, Swenson MJ, Reece WO. Fisiologia dos animais domésticos. Rio de Janeiro: Guanabara Koogan, 1993: 856 p.

Munguia R, Daniel SJ. Ototopical antifungals and otomycosis: a review. Int J Pediatr Otorhinolaryngol 2008; 72: 453–9.

Sargent SJ, Frank LA, Buchanan BR, Donnell RL, Morandi F. Otoscopic, cytological, and microbiological examination of the equine external ear canal. Vet Dermatol 2006; 17: 175–81.

Khosravi AR, Shokri H, Ziglari T, Niasari-Naslaji A. A study of mycoflora of the external ear canals in Dromedary camels in Iran. J Camel Pract Res 2008; 15: 155–9.

Hayyawi SM. Comparison of microbial isolates isolated from external ear canal of sheep and their susceptibility to antibiotics. In: Proceeding of the Eleventh Veterinary Scientific Conference, 2012: 41–8.

Tater KC, Scott DW, Miller Jr WH, Erb HN. The cytology of the external ear canal in the normal dog and cat. J Vet Med A 2003; 50: 370–4.

Quinton JF, Francois M, Laprais A, Prelaud P. Cytology of the external auditory meatus in healthy domestic pet rabbits (Oryctolagus cuniculus). Rev Méd Vét 2014; 165: 263–6.

Brotto TL, Andrade MCR, Goncalves MAB, Gimenis F, Pina A. Identification of fungi microflora in the ear conducts of rhesus macaques (Macaca mulatta) kept in captivity. Braz J Vet Res Anim Sci 2005; 42: 459–64.

Chinnadurai SK, Suedmeyer WK, Fales WH. Microbiology of the external ear canal in six African elephants (Loxodonta africana). Vet Rec 2009; 164: 238–40.

Duarte ER, Melo MM, Hamdan JS. Epidemiological aspects of bovine parasitic otitis caused by Rhabditis spp. and/or Raillietia spp. in the State of Minas Gerais, Brazil. Vet Parasitol 2001; 101: 45–52.

Lilenbaum W, Veras M, Blum E, Souza GN. Antimicrobial susceptibility of Staphylococci isolated from otitis externa in dogs. Lett Appl Microbiol 2000; 31: 42–5.

Barrasa JL, Gomez PL, Lama ZG, Junco MTJ. Antibacterial susceptibility patterns of Pseudomonas strains isolated from chronic canine otitis externa. J Vet Med B 2000; 47: 191–6.

Campbell JJ, Coyner KS, Rankin SC. Evaluation of fungal flora in normal and diseased canine ears. Vet Dermatol 2010; 21: 619–25.

Anaissie EJ, McGinnis MR, Pfaller MA. Clinical mycology. Philadelphia : Churchill Livingston, 2003: 149 p.

Klich MA. Identification of common Aspergillus species. Utrecht, Netherlands : Centraalbureau voor Schimmelcultures, 2002.

Leslie JF, Summerell BA. The Fusarium laboratory manual. Ames : Blackwell Publishing Professional, 2006.

Khosravi AR, Yarahmadi S, Baiat M, Shokri H, Pourkabireh M. Factors affecting the prevalence of yeasts in the oral cavity of patients with diabetes mellitus. J Mycol Med 2008; 18: 83–8.

Naeini AR, Nazeri M, Shokri H. Antifungal activity of Zataria multiflora, Pelargonium graveolens and Cuminum cyminum essential oils towards three species of Malassezia isolated from patients with pityriasis versicolor. J Mycol Med 2011; 21: 87–91.

Loeffler J, Schmidt K, Hebart H, Schumacher U, Einsele H. Automated extraction of genomic DNA from medically important yeast species and filamentous fungi by using the MagNA Pure LC system. J Clin Microbiol 2002; 40: 2240–3.

Duarte ER, Resende JCP, Rosa CA, Hamdan JS. Prevalence of yeasts and mycelial fungi in bovine parasitic otitis in the state of Minas Gerais, Brazil. J Vet Med B 2001; 48: 631–5.

Duarte ER, Melo MM, Hahn RC. Prevalence of Malassezia spp. in the ears of asymptomatic cattle and cattle with otitis in Brazil. Med Mycol 1999; 37: 159–62.

Oliveira LC, Leite CA, Brilhante RS. Comparative study of the microbial profile from bilateral canine otitis externa. Can Vet J 2008; 49: 785–8.

Gustafson BH. The occurrence of yeast belonging to genus Pityrosporum in different kinds of animals. Acta Pathol Microbiol Scand 1960; 48: 51–5.

Duarte ER, Batista RD, Hahn RC, Hamdan JS. Factors associated with the prevalence of Malassezia species in the external ears of cattle from the state of Minas Gerais, Brazil. Med Mycol 2003; 41: 137–42.

Dufait R. Pre´sence de Malassezia pachydermatis (syn. Pityrosporum canis) sur les poils et les plumes des animaux domestiques. Bull Soc Med Mycol 1985; 14: 19–20.

Guillot J, Chermette R, Guého E. Prevalénce du genre Malassezia chez les mammifères. J Med Mycol 1994; 4: 72–9.

Scott DW, Miller WH, Griffin CE. Fungal skin diseases. In: Muller & Kirk’s small animal dermatology. Philadelphia : W. B. Saunders, 2001: 338 p.

Samanta I. Veterinary mycology. New Delhi : Springer New Delhi, 2015: 88–9 p.

Shimada AT, Adachi T, Umemura K, Kohno Y, Sakaguchi C, Takura I. A pathologic and bacteriologic study on otitis media in swine. Vet Pathol 1992; 29: 337–42.

Henderson JP, Mccullough WP. Otitis media in suckler calves. Vet Rec 1993; 132: 24.




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