THE EFFICACY OF VACCINATION OF LAYER CHICKENS WITH INACTIVATED FOWL CHOLERA BACTERIN PREPARED FROM LOCAL EGYPTIAN STRAINS OF Pasteurella multocida

Wafaa Abd El-Ghany, Hanan Ali Ahmed, Ali Zaher Qandoos, Mohamed Abd El-Rahman Bosila Bosila

Abstract


This study was carried out to evaluate the efficacy of vaccination of layer chickens with inactivated FC bacterin prepared from local Egyptian strains of Pasteurella multocida (P. multocida). A total of 200 layer chickens were divided into 5 equal groups, 40 for each. At the age of 6 weeks, chickens in groups (A) and (B) were vaccinated with P. multocida serotypes A:1 and A:3, respectively, booster doses were given after 3 weeks (9 weeks old) and challenge was done with virulent serotypes A:1 and A:3 at 2 weeks later (11 weeks old). Chickens in groups (C) and (D) were not vaccinated, only challenged with P. multocida serotype A:1 and A:3, respectively. Birds in group (E) were kept as non-vaccinated and non-challenged. Blood samples were collected weekly from all groups for humoral immune response. All the birds were kept under observation for signs, mortalities, lesions and re-isolation of challenging organism and for histopathological examination. Results of the mean Enzyme Linked Immuno-Sorbent Assay (ELISA) revealed that the highest level was at 5 weeks post vaccination as the titers reached to 3970 in group (A) and 3905 in group (B). The clinical signs, mortality rate and lesions were mild in the vaccinated birds while severe lesions were in non-vaccinated and challenged birds. The protection rates were 85 % and 80 % in groups (A) and (B); respectively, while 10 % and 20 % in groups (C) and (D); respectively. The re-isolation rates of P. multocida after challenge were 95 % and 90 % in non-vaccinated-challenged birds with P. multocida serotypes A:1 and A:3; respectively, while they were 25 % and 15 % in vaccinated-challenged groups with P. multocida serotypes A:1 and A:3; respectively. Histopathological examination of P. multocida vaccinated-challenged birds revealed mild to no microscopic lesions when compared with non-vaccinated challenged chickens. In conclusion, the prepared FC inactivated bacterin from the local Egyptian predominant P. multocida serovars proved efficacy and protection of layer chickens.

 

Key words: Pasteurella multocida; chickens; immunization; protection; Egypt

 

UÄŒINKOVITOST CEPLJENJA KOKOÅ I NESNIC Z INAKTIVIRANO BAKTERIJO KOLERE PERJADI, PRIPRAVLJENE IZ LOKALNIH EGIPTOVSKIH SEVOV BAKTERIJE Pasteurella multocida

 

Povzetek: Raziskava je bila izvedena z namenom ocenitve uÄinkovitosti cepljenja kokoÅ¡i nesnic z inaktivirano bakterijo FC, pripravljeno iz lokalnih egiptovskih sevov bakterije Pasteurella multocida (P. multocida). Skupno 200 kokoÅ¡i nesnic je bilo razdeljenih v 5 enakih skupin. V vsaki skupini je bilo 40 kokoÅ¡i. Pri 6 tednih smo kokoÅ¡i v skupinah A in B cepili s serotipoma P. multocida A:1 in A:3, po 3 tednih, ko so bile živali stare 9 tednov, so dobile poživitvene doze cepiva. Po dveh tednih (v starosti 11 tednov) so bile kokoÅ¡i okužene z virulentnima serotipoma A:1 in A:3. PiÅ¡Äanci v skupinah C in D niso bili cepljeni temveÄ samo okuženi s serotipoma A:1 in A:3. KokoÅ¡i v skupini E niso bile niti cepljene, niti okužene. Vzorci krvi so bili odvzeti pri vseh skupinah tedensko za preverjanje humoralnega imunskega odziva. Vse kokoÅ¡i smo stalno opazovali in beležili prisotnost bolezenskih znakov, razliÄnih ran in umiranje kokoÅ¡i. Pri poginulih kokoÅ¡ih smo osamili bakterije ter opravili histopatoloÅ¡ki pregled. Rezultati encimsko-imunskega testa (ELISA) so pokazali da je bila najviÅ¡ja stopnja zaÅ¡Äite dosežena 5 tednov po cepljenju, saj so titri dosegli 3970 v skupini A in 3905 v skupini B. KliniÄni znaki, stopnja umrljivosti in rane so bili pri cepljenih kokoÅ¡ih blagi, hude rane pa so bile vidne pri necepljenih in okuženih kokoÅ¡ih. Stopnja zaÅ¡Äite je bila v skupinah A in B 85- oziroma 80-odstotna, v skupinah C in D pa 10- oziroma 20-odstotna. Stopnje ponovne izolacije P. multocida po okužbi so bile 90 in 95 odstotkov pri kokoÅ¡ih, ki niso bile cepljene, medtem, ko so bile v skupinah, ki so bile okužene s P. multocida serotipa A:1 in A:3 15- in 25-odstotkov. HistopatoloÅ¡ki pregled cepljenih in okuženih kokoÅ¡i je pokazal popolno odsotnost ali prisotnost blagih mikroskopskih poÅ¡kodb, medtem ko so imele necepljene okužene kokoÅ¡i bolj obsežne histopatoloÅ¡ke poÅ¡kodbe. Pripravljena inaktivirana bakterija FC iz lokalnih egiptovskih prevladujoÄih serovarov P. multocide se je izkazala za uÄinkovito zaÅ¡Äito kokoÅ¡i nesnic.

KljuÄne besede: Pasteurella multocida; kokoÅ¡i; imunizacija; zaÅ¡Äita; Egipt


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DOI: https://doi.org/10.26873/SVR-1000-2020

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